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Clara L. Kielkopf, Ph.D.

Contact Information

Phone Numbers

Office: (585) 273-4799

Fax: (585) 271-2683

Research Labs

Faculty Appointments



The Kielkopf lab investigates the molecular structures and interactions among key pre-mRNA splicing factors in human genetic diseases and hematologic malignancies (Trends Genetics 2017). Our recent structures of U2AF2 bound to a range of splice site signals provide a basis for understanding disease-causing mutations in the splice sites of gene transcripts (PNAS 2014; Nature Commun. 2016). As a step towards viewing the architecture of multi-subunit pre-mRNA splicing factor complexes, we revealed phosphorylation-induced conformational changes in the splicing factor subunit SF1 bound to U2AF2, and demonstrated that the phosphorylated interface is essential in vivo (Structure 2013). We have further shown that recurrent, cancer-associated mutations of U2AF1 alter its RNA interactions (Leukemia 2014; PLOS Genetics 2017). Now, our immediate goal is to determine roadmaps of the key interactions among higher order 3' splice site complexes, and in future phases of the project, assist the development of therapeutics targeted at the level of pre-mRNA splicing.

We are grateful to the following organizations for research funding:

National Institutes of Health:
R01 GM070503: Molecular Recognition During pre-mRNA Splicing
R01 GM117005: Structural Control of Human Co-factors for Retroviral Gene Expression

Edward P. Evans Foundation:
Research Discovery Grant: Structural mechanisms & targeting of MDS-relevant pre-mRNA splicing factors



B.S. | University of Wisconsin-Madison
Molecular Biology (summa cum laude)

Ph.D. | California Institute of Technology
Biology and Chemistry

Post-doctoral Training & Residency

2000 - 2001
Post-doctoral Training | The Rockefeller University


2020 - 2023
Edward P. Evans Foundation Research Award

2017 - 2019
Edward P. Evans Foundation Research Award

2014 - 2016
Edward P. Evans Foundation Research Award

Cover illustration for CSHL mRNA processing meeting abstract book

Prostate Cancer Foundation Award

Kimmel Scholar Cancer Research Award

March of Dimes Basil O'Connor Award

2000 - 2002
American Cancer Society Postdoctoral fellowship

1994 - 1997
NSF Pre-Doctoral Fellowship

Hilldale Fellowship, University of Wisconsin-Madison

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Journal Articles

Galardi JW, Bela VN, Jeffery N, He X, Glasser E, Loerch S, Jenkins JL, Pulvino MJ, Boutz PL, Kielkopf CL. "A UHM - ULM interface with unusual structural features contributes to U2AF2 and SF3B1 association for pre-mRNA splicing." The Journal of biological chemistry.. 2022 Jun 30; :102224. Epub 2022 Jun 30.

Glasser E, Maji D, Biancon G, Puthenpeedikakkal AMK, Cavender CE, Tebaldi T, Jenkins JL, Mathews DH, Halene S, Kielkopf CL. "Pre-mRNA splicing factor U2AF2 recognizes distinct conformations of nucleotide variants at the center of the pre-mRNA splice site signal." Nucleic acids research.. 2022 May 7; Epub 2022 May 07.

Chatrikhi R, Feeney CF, Pulvino MJ, Alachouzos G, MacRae AJ, Falls Z, Rai S, Brennessel WW, Jenkins JL, Walter MJ, Graubert TA, Samudrala R, Jurica MS, Frontier AJ, Kielkopf CL. "A synthetic small molecule stalls pre-mRNA splicing by promoting an early-stage U2AF2-RNA complex." Cell chemical biology.. 2021 Feb 26; Epub 2021 Feb 26.