Paul Kingsley, Ph.D.
Contact
Paul Kingsley, Ph.D.
About Me
Faculty Appointments
Research Assistant Professor - Department of Pediatrics, Hematology and Oncology (SMD)
Credentials
Post-doctoral Training & Residency
Cancer Center Fellowship in Hematopoiesis, University of Rochester School of Medicine and Dentistry, Rochester, NY 1992 - 1994
Education
PhD | University of Rochester. Developmental Biology. 1992
MS | University of Rochester. Developmental Biology. 1987
BA | Reed College. Biology. 1982
Research
1) Embryonic Hematopoiesis
2) Erythroid Differentiation
3) Ontogeny of Hematopoietic Progenitors
4) Erythroid Precursor Self Renewal
Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Forma...
1) Embryonic Hematopoiesis
2) Erythroid Differentiation
3) Ontogeny of Hematopoietic Progenitors
4) Erythroid Precursor Self Renewal
Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Formation of blood is a critical early event for mammalian embryos, initially occurring in the extraembryonic yolk sac of the gastrulating embryo, then migrating to the fetal liver, and finally homing to the bone marrow at the end of gestation. They are using subtractive hybridization and degenerate PCR to identify gene family members involved in specification, expansion, mobilization and migration of hematopoietic stem cells and progenitors.
An alternate approach they use is genechip technology (Affymetrix), where tens of thousands of gene products are evaluated quantitatively for expression in different regions of the developing embryo. Target tissues include microdissected regions from early murine embryos as well as embryonic cells triple-sorted for known hematopoietic/angiogenic surface markers. The spatial and temporal expression patterns of candidate genes are confirmed by in situ hybridization to both whole embryos and sections of embryonic tissues. The function of candidate molecules are tested using a variety of in vitro and in vivo model systems.
Publications
Journal Articles
Livada AC, McGrath KE, Malloy MW, Li C, Ture SK, Kingsley PD, Koniski AD, Vit LA, Nolan KE, Mickelsen D, Monette GE, Maurya P, Palis J, Morrell CN
The Journal of clinical investigation.. 2024 September 19 Epub 09/19/2024.
McGrath KE, Koniski AD, Murphy K, Getman M, An HH, Schulz VP, Kim AR, Zhang B, Schofield TL, Papoin J, Blanc L, Kingsley PD, Westhoff CM, Gallagher PG, Chou ST, Steiner LA, Palis J
bioRxiv : the preprint server for biology.. 2024 February 6 Epub 02/06/2024.
Nemkov T, Kingsley PD, Dzieciatkowska M, Malik J, McGrath K, Hansen KC, D'Alessandro A, Palis J
Blood advances.. 2022 May 246 (10):3072-3089. Epub 1900 01 01.
Saunders J, Niswander LM, McGrath KE, Koniski A, Catherman SC, Ture SK, Medhora M, Kingsley PD, Calvi LM, Williams JP, Morrell CN, Palis J
Radiation research.. 2021 September 1196 (3):284-296. Epub 1900 01 01.
Zhang Y, McGrath KE, Ayoub E, Kingsley PD, Yu H, Fegan K, McGlynn KA, Rudzinskas S, Palis J, Perkins AS
Cell reports.. 2021 August 1736 (7):109562. Epub 1900 01 01.
Jeffery NN, Davidson C, Peslak SA, Kingsley PD, Nakamura Y, Palis J, Bulger M
Epigenetics & chromatin.. 2021 July 3014 (1):37. Epub 07/30/2021.
Cao J, O'Day DR, Pliner HA, Kingsley PD, Deng M, Daza RM, Zager MA, Aldinger KA, Blecher-Gonen R, Zhang F, Spielmann M, Palis J, Doherty D, Steemers FJ, Glass IA, Trapnell C, Shendure J
Science.. 2020 November 13370 (6518)Epub 1900 01 01.
Fox S, Myers JA, Davidson C, Getman M, Kingsley PD, Frankiewicz N, Bulger M
Nature communications.. 2020 September 1111 (1):4544. Epub 09/11/2020.
Dege C, Fegan KH, Creamer JP, Berrien-Elliott MM, Luff SA, Kim D, Wagner JA, Kingsley PD, McGrath KE, Fehniger TA, Palis J, Sturgeon CM
Developmental cell.. 2020 April 2053 (2):229-239.e7. Epub 03/19/2020.
Calvi LM, Frisch BJ, Kingsley PD, Koniski AD, Love TM, Williams JP, Palis J
International journal of radiation biology.. 2019 November 95 (11):1447-1461. Epub 07/30/2019.
Stolla MC, Catherman SC, Kingsley PD, Rowe RG, Koniski AD, Fegan K, Vit L, McGrath KE, Daley GQ, Palis J
Blood advances.. 2019 January 83 (1):72-82. Epub 1900 01 01.
Yien YY, Shi J, Chen C, Cheung JT, Grillo AS, Shrestha R, Li L, Zhang X, Kafina MD, Kingsley PD, King MJ, Ablain J, Li H, Zon L, Palis J, Burke MD, Bauer DE, Orkin SH, Koehler CM, Phillips JD, Kaplan J, Ward DM, Lodish HF, Paw BH
The Journal of biological chemistry.. 2018 December 21293 (51):19797-19811. Epub 10/26/2018.
Zhou S, Huang YS, Kingsley PD, Cyr KH, Palis J, Wan J
Biomicrofluidics.. 2017 September 11 (5):054112. Epub 10/23/2017.
Huang YS, Delgadillo LF, Cyr KH, Kingsley PD, An X, McGrath KE, Mohandas N, Conboy JG, Waugh RE, Wan J, Palis J
Scientific reports.. 2017 July 127 (1):5164. Epub 07/12/2017.
Liddicoat BJ, Hartner JC, Piskol R, Ramaswami G, Chalk AM, Kingsley PD, Sankaran VG, Wall M, Purton LE, Seeburg PH, Palis J, Orkin SH, Lu J, Li JB, Walkley CR
Experimental hematology.. 2016 October 44 (10):947-63. Epub 07/01/2016.
McGrath KE, Frame JM, Fegan KH, Bowen JR, Conway SJ, Catherman SC, Kingsley PD, Koniski AD, Palis J
Cell reports.. 2015 June 3011 (12):1892-904. Epub 06/18/2015.
Kim AR, Olsen JL, England SJ, Huang YS, Fegan KH, Delgadillo LF, McGrath KE, Kingsley PD, Waugh RE, Palis J
Stem cell reports.. 2015 June 94 (6):995-1003. Epub 05/28/2015.
Chung J, Bauer DE, Ghamari A, Nizzi CP, Deck KM, Kingsley PD, Yien YY, Huston NC, Chen C, Schultz IJ, Dalton AJ, Wittig JG, Palis J, Orkin SH, Lodish HF, Eisenstein RS, Cantor AB, Paw BH
Science signaling.. 2015 April 148 (372):ra34. Epub 04/14/2015.
Niswander LM, Fegan KH, Kingsley PD, McGrath KE, Palis J
Blood.. 2014 July 10124 (2):277-86. Epub 04/15/2014.
Kingsley PD, Greenfest-Allen E, Frame JM, Bushnell TP, Malik J, McGrath KE, Stoeckert CJ, Palis J
Blood.. 2013 February 7121 (6):e5-e13. Epub 12/12/2012.
Peslak SA, Wenger J, Bemis JC, Kingsley PD, Koniski AD, McGrath KE, Palis J
Blood.. 2012 September 20120 (12):2501-11. Epub 08/13/2012.
Fromm G, Cadiz-Rivera B, de Vries C, Getman M, McGrath KE, Kingsley PD, Fields J, Fiering S, Bulger M
Blood.. 2011 May 12117 (19):5207-14. Epub 02/14/2011.
McGrath KE, Frame JM, Fromm GJ, Koniski AD, Kingsley PD, Little J, Bulger M, Palis J
Blood.. 2011 April 28117 (17):4600-8. Epub 03/04/2011.
Peslak SA, Wenger J, Bemis JC, Kingsley PD, Frame JM, Koniski AD, Chen Y, Williams JP, McGrath KE, Dertinger SD, Palis J
Experimental hematology.. 2011 April 39 (4):434-45. Epub 02/01/2011.
Palis J, Malik J, McGrath KE, Kingsley PD
The International journal of developmental biology.. 2010 54 (6-7):1011-8. Epub 1900 01 01.
Nilsson R, Schultz IJ, Pierce EL, Soltis KA, Naranuntarat A, Ward DM, Baughman JM, Paradkar PN, Kingsley PD, Culotta VC, Kaplan J, Palis J, Paw BH, Mootha VK
Cell metabolism.. 2009 August 10 (2):119-30. Epub 1900 01 01.
Reduced COX-2 expression in aged mice is associated with impaired fracture healing.
Naik, A.A.; Xie, C.; Zuscik, M.J.; Kingsley, P., Schwarz, E.M.; Awad, H.; Guldberg, R.; Drissi, H.; Puzas, J.E.; Boyce, B.; Zhang, X; O'Keefe, R.J.
J Bone Miner Res. 2009; 24: 251-264.
Bullard T, Koek L, Roztocil E, Kingsley PD, Mirels L, Ovitt CE
Developmental biology.. 2008 August 1320 (1):72-8. Epub 04/23/2008.
McGrath KE, Kingsley PD, Koniski AD, Porter RL, Bushnell TP, Palis J
Blood.. 2008 February 15111 (4):2409-17. Epub 11/21/2007.
Keel SB, Doty RT, Yang Z, Quigley JG, Chen J, Knoblaugh S, Kingsley PD, De Domenico I, Vaughn MB, Kaplan J, Palis J, Abkowitz JL
Science.. 2008 February 8319 (5864):825-8. Epub 1900 01 01.
Kingsley PD, Malik J, Emerson RL, Bushnell TP, McGrath KE, Bloedorn LA, Bulger M, Palis J
Blood.. 2006 February 15107 (4):1665-72. Epub 11/01/2005.
Yi Z, Cohen-Barak O, Hagiwara N, Kingsley PD, Fuchs DA, Erickson DT, Epner EM, Palis J, Brilliant MH
PLoS genetics.. 2006 February 2 (2):e14. Epub 02/03/2006.
Wingert RA, Galloway JL, Barut B, Foott H, Fraenkel P, Axe JL, Weber GJ, Dooley K, Davidson AJ, Schmid B, Schmidt B, Paw BH, Shaw GC, Kingsley P, Palis J, Schubert H, Chen O, Kaplan J, Zon LI,
Nature.. 2005 August 18436 (7053):1035-39. Epub 1900 01 01.
Smith N, Dong Y, Lian JB, Pratap J, Kingsley PD, van Wijnen AJ, Stein JL, Schwarz EM, O'Keefe RJ, Stein GS, Drissi MH
Journal of cellular physiology.. 2005 April 203 (1):133-43. Epub 1900 01 01.
Kingsley PD, Malik J, Fantauzzo KA, Palis J
Blood.. 2004 July 1104 (1):19-25. Epub 03/18/2004.
The zebrafish moonshine gene encodes transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesis
Ransom,D. G.; Bahary,N.; Niss,K.; Traver,D.; Burns,C.; Trede,N. S.; Paffett-Lugassy,N.; Saganic,W. J.; Lim,C. A.; Hersey,C.; Zhou,Y.; Barut,B. A.; Lin,S.; Kingsley,P. D.; Palis,J.; Orkin,S. H.; Zon,L. I.;.
PLoS Biol. 2004; 2(8): E237.
Davidson AJ, Ernst P, Wang Y, Dekens MP, Kingsley PD, Palis J, Korsmeyer SJ, Daley GQ, Zon LI
Nature.. 2003 September 18425 (6955):300-6. Epub 1900 01 01.
Chung YS, Zhang WJ, Arentson E, Kingsley PD, Palis J, Choi K
Development.. 2002 December 129 (23):5511-20. Epub 1900 01 01.
Lacaud G, Gore L, Kennedy M, Kouskoff V, Kingsley P, Hogan C, Carlsson L, Speck N, Palis J, Keller G
Blood.. 2002 July 15100 (2):458-66. Epub 1900 01 01.
Elble RC, Ji G, Nehrke K, DeBiasio J, Kingsley PD, Kotlikoff MI, Pauli BU
The Journal of biological chemistry.. 2002 May 24277 (21):18586-91. Epub 03/14/2002.
Lan Y, Kingsley PD, Cho ES, Jiang R
Mechanisms of development.. 2001 September 107 (1-2):175-9. Epub 1900 01 01.
Ten Hagen KG, Bedi GS, Tetaert D, Kingsley PD, Hagen FK, Balys MM, Beres TM, Degand P, Tabak LA
The Journal of biological chemistry.. 2001 May 18276 (20):17395-404. Epub 02/06/2001.
Kingsley PD, McGrath KE, Maltby KM, Koniski AD, Ramchandran R, Palis J
Development, growth & differentiation.. 2001 April 43 (2):133-43. Epub 1900 01 01.
Kingsley PD, Hagen KG, Maltby KM, Zara J, Tabak LA
Glycobiology.. 2000 December 10 (12):1317-23. Epub 1900 01 01.
Chi H, Yang X, Kingsley PD, O'Keefe RJ, Puzas JE, Rosier RN, Shears SB, Reynolds PR
Molecular and cellular biology.. 2000 September 20 (17):6496-507. Epub 1900 01 01.
Donovan A, Brownlie A, Zhou Y, Shepard J, Pratt SJ, Moynihan J, Paw BH, Drejer A, Barut B, Zapata A, Law TC, Brugnara C, Lux SE, Pinkus GS, Pinkus JL, Kingsley PD, Palis J, Fleming MD, Andrews NC, Zon LI
Nature.. 2000 February 17403 (6771):776-81. Epub 1900 01 01.
Liu LQ, Ilaria R, Kingsley PD, Iwama A, van Etten RA, Palis J, Zhang DE
Molecular and cellular biology.. 1999 April 19 (4):3029-38. Epub 1900 01 01.
Hetherington CJ, Kingsley PD, Crocicchio F, Zhang P, Rabin MS, Palis J, Zhang DE
The Journal of immunology : official journal of the American Association of Immunologists.. 1999 January 1162 (1):503-9. Epub 1900 01 01.
Characterization of human endotoxin lipopolysaccharide receptor CD14 expression in transgenic mice.
Hetherington, C.J.; Kingsley, P.D.; Crocicchio, F.; Zhang, P.; Rabin, M.S.; Palis, J.; Zhang, D.E.
J Immunol. 1999; 163(1): 503.
Kingsley PD, Whitin JC, Cohen HJ, Palis J
Molecular reproduction and development.. 1998 April 49 (4):343-55. Epub 1900 01 01.
Varade WS, Carnahan JA, Kingsley PD, Insel RA
Immunology.. 1998 February 93 (2):171-6. Epub 1900 01 01.
Miller RN, Dalamagas DG, Kingsley PD, Ettensohn CA
Mechanisms of development.. 1996 November 60 (1):3-12. Epub 1900 01 01.
Primary structure and developmental regulation of murine CNBP, a zinc finger-containing protein whose general structure is present in evolutionarily diverse eukaryotes
Kingsley PD, Zinkin AS, Silver LS, Pendola FL, Palis J.
Dev. Growth Diff. 1996; 38: 697-706.
Palis J, Kingsley PD
Molecular reproduction and development.. 1995 September 42 (1):19-27. Epub 1900 01 01.
Palis J, McGrath KE, Kingsley PD
Blood.. 1995 July 186 (1):156-63. Epub 1900 01 01.
Kingsley PD, Palis J
The Plant cell.. 1994 November 6 (11):1522-3. Epub 1900 01 01.
Yang Q, Kingsley PD, Kozlowski DJ, Angerer RC, Angerer LM
Development, growth & differentiation.. 1993 April 35 (2):139-151. Epub 1900 01 01.
Kingsley PD, Angerer LM, Angerer RC
Developmental biology.. 1993 January 155 (1):216-34. Epub 1900 01 01.
Immunochemical analysis of arylsulfatase accumulation in sea urchin embryos
Yang Q, Kingsley PD, Kozlowski RC, Angerer RC, Angerer LM.
Dev Growth Differ. 1993; 35: 139-151.
Angerer LM, Yang Q, Liesveld J, Kingsley PD, Angerer RC
Developmental biology.. 1992 January 149 (1):27-40. Epub 1900 01 01.
Contributions of the spatial analysis of gene expression to the study of sea urchin development
Angerer RC, Reynolds SD, Grimwade J, Hurley DL, Yang Q, Kingsley PD, Gagnon ML, Palis J, Angerer LM.
Soc Exp Biol Seminars (Cambridge Press). 1990; 43: 69-95.
Human trabecular meshwork organ culture: morphology and glycosaminoglycan synthesis
Acott TS, Kingsley PD, Samples JR, Van Buskirk EM.
Inv Opthal Vis Sci. 1988; 29((1)): 90-100.
Daley ML, Gallo AE, Gehling GF, Isom JB, Mauch W, Kingsley PD
Neurosurgery.. 1982 November 11 (5):617-21. Epub 1900 01 01.