2:30pm - 3:30pm|
Department of Microbiology and Immunology Seminar Series
First Annual MBI Alumni Seminar
Steven Baker, Ph.D.
University of Wisconsin-Madison
Steven was a student in Luis Martinez-Sobrido's laboratory. He received his Ph.D. in 2015
"Adaptation of influenza virus polymerase to the divergent host protein ANP32A"
Thursday, November 10, 2016
Upper Auditorium (Room 3-7619)
Seminar Abstract: The influenza virus RNA-dependent RNA polymerase directs viral genome transcription and replication by co-opting host cell proteins. Although influenza viruses circulate in diverse animal species, specific amino acid variants confer replicative fitness in one host versus another. One such site that toggles host species specificity is residue 627 of the polymerase subint PB2 (PB2-627). Viruses encoding polymerases with a lysine at PB2-627 replicate efficiently in mammalian and avian hosts, but the glutamic acid common at PB2-627 from avian isolates restricts replication to avian hosts. Restriction is overcome, however, by overexpressing avian ANP32A in mammalian cells. This seminar will address several questions that remain unanswered by these observations: 1) what stage of polyemrase function is regulated by ANP32A, 2) how does the unique avian isoform of ANP32A control PB2-627E, and 3) if PB2-627K is functional in mammalian and avian cells, why does PB2-627E persist?pre-vaccination gene expression signatures that bias the infection or the vaccine response to a positive or a negative outcome. We will show that the presence of specific interferon signatures prior to infection or prior to vaccination can tilt the balance towards a positive or a negative outcome. This work is supported by BMGF , U19 AI095985and U19 U19 AI096109
|Location: ||Upper Auditorium (3-7619)|
Posted by: Brenda Knorr, Micro & Immunolgy, 18-Oct-16 10:31am ET