Does this test have other names?
Testosterone (total), serum testosterone, free testosterone
What is this test?
This test measures the level of the hormone testosterone in your blood. Testosterone
is a male sex hormone (androgen) that helps male features develop. Testosterone is
made in the testes in males, the ovaries in females, and in the adrenal glands.
Testosterone causes the changes that occur in boys during puberty. It helps hair,
muscles, penis, and testes to grow. Testosterone also causes a boy's voice to deepen.
Males continue to make testosterone. In adult males, it boosts sex drive and helps
the sperm mature.
In females, the ovaries make small amounts of testosterone. It helps many organs and
body processes in females.
The pituitary gland in your brain controls the amount of testosterone your body makes.
It does this by using other signaling hormones such as luteinizing hormone.
Most of the testosterone in the blood attaches to 2 proteins: albumin and sex hormone
binding globulin (SHBG). Some testosterone is not attached to proteins. This testosterone
is called free testosterone. Free testosterone and albumin-bound testosterone are
also referred to as bioavailable testosterone. This is the testosterone that is easily
used by your body.
If your healthcare provider thinks that you have low or high testosterone, they will
first test total testosterone levels. This looks at all 3 types of testosterone. The
free testosterone can help give more information when total testosterone is low.
Both males and females can have health problems because of low or high levels of testosterone.
Females with high levels of testosterone may have polycystic ovary syndrome. This
condition can cause:
Testosterone levels in males drop as they age. This is not considered to be hypogonadism.
The FDA currently advises against treating males with low testosterone caused only
Why do I need this test?
You may need this test if you have symptoms of low testosterone.
Symptoms of low testosterone in males include:
Low sex drive or lack of interest in sex
Trouble getting an erection
Low sperm count and other fertility problems
Changes in the testicles
Loss of muscle mass
Symptoms of low testosterone in females include:
What other tests might I have along with this test?
You may also have other blood tests to check hormone levels. These include:
Follicle-stimulating hormone (FSH) test
Luteinizing hormone (LH) test
Thyroid stimulating hormone (TSH) test
You may also need to have:
What do my test results mean?
Test results may vary depending on your age, gender, health history, and other things.
Your test results may be different depending on the lab used. They may not mean you
have a problem. Ask your healthcare provider what your test results mean for you.
The results of this test are given in nanograms per deciliter (ng/dL). Normal test
results show total testosterone levels of:
If your testosterone levels are higher or lower than normal, you may have a condition
that affects your testosterone production. If your levels are higher than normal,
you may have a tumor on the testes or ovaries.
How is this test done?
The test is done with a blood sample. A needle is used to draw blood from a vein in
your arm or hand. This test is usually done in the morning. This is because testosterone
levels tend to be highest at that time. But you may need to have this test more than
once, and at different times of the day, to confirm low testosterone levels. This
is because your testosterone level can change from morning to evening and from day
Does this test pose any risks?
Having a blood test with a needle carries some risks. These include bleeding, infection,
bruising, and feeling lightheaded. When the needle pricks your arm or hand, you may
feel a slight sting or pain. Afterward, the site may be sore.
What might affect my test results?
Some medicines may affect your test results. These include antifungal medicines such
as ketoconazole and hormone medicines. Some foods that have phytoestrogen can also
affect the testosterone levels. These include some fruits and vegetables, wine, and
tea. Having the test done late in the day may show that your testosterone level is
lower than it really is.
How do I get ready for this test?
You don't need to prepare for this test. But be sure your healthcare provider knows
about all medicines, herbs, vitamins, and supplements you are taking. This includes
medicines that don't need a prescription and any illegal drugs you may use.