Does this test have other names?
25-hydroxyvitamin D (25-high-DROX-ee-VIE-tuh-min D), 25(OH)D
What is this test?
Vitamin D is mainly found in fortified dairy foods, juice, breakfast cereal, and certain
fish. This vitamin plays many roles in the body. But because it helps the body absorb
calcium from foods and supplements, it's particularly important for bone health. Vitamin
D has many additional roles in the body.
Vitamin D comes in several forms. When ultraviolet light, such as sunlight, hits your
skin, it creates vitamin D3. D2 is used to fortify dairy foods. Both of these are
further processed by your liver and kidneys into a form your body can use. Most tests
for vitamin D check the level of a form circulating in the body called 25-hydroxyvitamin
D, also called 25(OH)D.
Why do I need this test?
You may need this test if your healthcare provider wants to check your vitamin D levels
to find out if you have any risks to bone health. These might be:
You may also need this test if you are at risk for low vitamin D levels. Risks include:
Being an older adult
Having difficulty absorbing fat from your diet
Having chronic kidney disease
Have dark skin pigmentation
Being a breastfed baby
Vitamin D has many effects in the body. You may need this test to help your healthcare
provider diagnose or treat:
Problems with the parathyroid gland
Autoimmune diseases, such as multiple sclerosis and inflammatory bowel disease
Weakness or falls
What other tests might I have along with this test?
A healthcare provider may also want to check your parathyroid hormone levels and your
What do my test results mean?
Test results may vary depending on your age, gender, health history, and other things.
Your test results may be different depending on the lab used. They may not mean you
have a problem. Ask your healthcare provider what your test results mean for you.
Although exact levels have not been identified, experts believe that levels of 20
nanograms per milliliter (ng/mL) or higher are enough for good bone and overall health.
Recommended daily amounts range from 400 to 800 international units (IU) per day based
on your age.
Levels lower than normal can mean you are:
Not making enough vitamin D on your own
Not getting enough vitamin D in your diet
Not absorbing vitamin D from your food as you should
Lower levels may also mean that your body is not converting the vitamin as it should.
This might be because of kidney or liver disease.
Above-normal levels may be a sign that you're taking too much in supplement form.
How is this test done?
The test is done with a blood sample. A needle is used to draw blood from a vein in
your arm or hand.
Does this test pose any risks?
Having a blood test with a needle carries some risks. These include bleeding, infection,
bruising, and feeling lightheaded. When the needle pricks your arm or hand, you may
feel a slight sting or pain. Afterward, the site may be sore.
What might affect my test results?
Your age, the amount of time you spend in the sunlight, your diet, and whether you
take vitamin D in supplement form can affect your vitamin D levels. Ask your healthcare
provider if any health conditions you have or medicines you take could affect your
How do I get ready for this test?
Tell your healthcare provider if you take vitamin D supplements. Be sure your healthcare
provider knows about all medicines, herbs, vitamins, and supplements you are taking.
This includes medicines that don't need a prescription and any illegal drugs you may