NMD is a splicing- and translation-dependent pathway that targets not only disease-associated transcripts but also naturally occurring transcripts (reviewed in Maquat et al., 2010, Cell 142:368-74). Many NMD targets are mistakes made during alternative splicing (Pan et al., 2006, Genes & Dev. 20:153-8). Currently, we are interested in further characterizing the pioneer round of translation, during which nonsense codon recognition leads to NMD, and how it differs from the bulk of cellular translation (Ishigaki et al., 2001 Cell 106:607-617; Lejeune et al., 2002, EMBO J. 21:3536-3545; Woeller et al., 2008, EMBO Rep. 9:446-451)
Learn more about Mechanisms of Nonsense-Mediated mRNA Decay (NMD)
We have found that STAU1, which is a double-stranded RNA binding protein, recruits the NMD factor UPF1 to certain mRNA 3'-untranslated regions (3'UTRs) so as to elicit SMD in a translation-dependent mechanism (reviewed in Park and Maquat, 2013, Wiley Interdiscip Rev RNA 4:423-435). Using microarray analyses, we have identified a number of mRNAs that are naturally down-regulated by SMD.
Learn more about Post-transcriptional Gene Regulation by Double-stranded RNA-binding Proteins