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URMC / Labs / Dirksen Lab / Techniques

Techniques

We employ several techniques in our laboratory including the following:

Electrophysiological Recording

  • Whole-cell voltage and current patch-clamp technique
  • Single channel patch-clamp recording
  • Perforated patch-clamp recording
  • Artificial lipid bilayer single channel recording

Molecular, biochemical, and cell biological techniques:

  • Molecular cloning
  • Western blotting
  • Immunoprecipitation
  • Immunostaining
  • RT-PCR
  • Transgenic gene expression

Optical/Imaging techniques

  • Bi-molecular fluorescence complementation
  • Fluorescence energy resonance transfer
  • Confocal imaging
  • Electron microscopy

Physiological assays

  • In vitro muscle contraction
  • In vivo muscle performance assays