Chromatin Structure Affects the Activity of DNA-Modifying Enzymes

Project Collaborator:

Dr. Robert Bambara

H1 binding affects uracil DNA glycosylase activity on sites within
the nucleosome. Bases affected upon H1 binding are indicated by red spheres.
Taken from Cole et al., 2010.

We are investigating how chromatin structure effects the activity of DNA-modifying enzymes involved in DNA replication and base-excision repair (BER). Specifically, we have found that two enzymes involved in these processes, human DNA ligase I and FEN1, are able to operate surprisingly efficiently on nucleosome substrates. We are currently investigating the mechanism of these enzymes and other components of the BER pathways.

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