URMC / Labs / Kielkopf Lab / Publications Recent Publications Kielkopf CL (2017 Nov 09). Insights from structures of cancer-relevant pre-mRNA splicing factors. Current opinion in genetics & development. Jenkins JLKielkopf CL (2017 Mar 31). Splicing Factor Mutations in Myelodysplasias: Insights from Spliceosome Structures. Trends in genetics : TIG. Chatrikhi RWang WGupta ALoerch SMaucuer AKielkopf CL (2016 Dec 20). SF1 Phosphorylation Enhances Specific Binding to U2AF(65) and Reduces Binding to 3'-Splice-Site RNA. Biophysical journal. Loerch SKielkopf CL (2016 Dec). Unmasking the U2AF homology motif family: a bona fide protein-protein interaction motif in disguise. RNA (New York, N.Y.). Fei DLMotowski HChatrikhi RPrasad SYu JGao SKielkopf CLBradley RKVarmus H (2016 Oct). Wild-Type U2AF1 Antagonizes the Splicing Program Characteristic of U2AF1-Mutant Tumors and Is Required for Cell Survival. PLoS genetics. Agrawal AASalsi EChatrikhi RHenderson SJenkins JLGreen MRErmolenko DNKielkopf CL (2016). An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal. Nature communications. Loerch SKielkopf CL (2015 Sep 25). Dividing and Conquering the Family of RNA Recognition Motifs: A Representative Case Based on hnRNP L. Journal of molecular biology. Okeyo-Owuor TWhite BSChatrikhi RMohan DRKim SGriffith MDing LKetkar-Kulkarni SHundal JLaird KMKielkopf CLLey TJWalter MJGraubert TA (2015 Apr). U2AF1 mutations alter sequence specificity of pre-mRNA binding and splicing. Leukemia. Agrawal AAMcLaughlin KJJenkins JLKielkopf CL (2014 Dec 9). Structure-guided U2AF65 variant improves recognition and splicing of a defective pre-mRNA. Proceedings of the National Academy of Sciences of the United States of America. Loerch SMaucuer AManceau VGreen MRKielkopf CL (2014 Jun 20). Cancer-relevant splicing factor CAPERα engages the essential splicing factor SF3b155 in a specific ternary complex. The Journal of biological chemistry. Gleghorn MLGong CKielkopf CLMaquat LE (2013 Apr). Staufen1 dimerizes through a conserved motif and a degenerate dsRNA-binding domain to promote mRNA decay. Nature structural & molecular biology. Jenkins JLAgrawal AAGupta AGreen MRKielkopf CL (2013 Apr 1). U2AF65 adapts to diverse pre-mRNA splice sites through conformational selection of specific and promiscuous RNA recognition motifs. Nucleic acids research. Wang WMaucuer AGupta AManceau VThickman KRBauer WJKennedy SDWedekind JEGreen MRKielkopf CL (2013 Feb 5). Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure (London, England : 1993). Jenkins JLLaird KMKielkopf CL (2012 Jul 3). A Broad range of conformations contribute to the solution ensemble of the essential splicing factor U2AF(65). Biochemistry. Bauer WJHeath JJenkins JLKielkopf CL (2012 Jan 27). Three RNA recognition motifs participate in RNA recognition and structural organization by the pro-apoptotic factor TIA-1. Journal of molecular biology. Gupta AKielkopf CL (2011 Apr 1). Purification, crystallization and preliminary X-ray crystallographic analysis of a central domain of human splicing factor 1. Acta crystallographica. Section F, Structural biology and crystallization communications. McLaughlin KJJenkins JLKielkopf CL (2011 Mar 8). Large favorable enthalpy changes drive specific RNA recognition by RNA recognition motif proteins. Biochemistry. Gupta AJenkins JLKielkopf CL (2011 Feb 4). RNA induces conformational changes in the SF1/U2AF65 splicing factor complex. Journal of molecular biology. McLaughlin KJStrain-Damerell CMXie KBrekasis DSoares ASPaget MSKielkopf CL (2010 May 28). Structural basis for NADH/NAD+ redox sensing by a Rex family repressor. Molecular cell. Jenkins JLShen HGreen MRKielkopf CL (2008 Nov 28). Solution conformation and thermodynamic characteristics of RNA binding by the splicing factor U2AF65. The Journal of biological chemistry. Manceau VKielkopf CLSobel AMaucuer A (2008 Sep 5). Different requirements of the kinase and UHM domains of KIS for its nuclear localization and binding to splicing factors. Journal of molecular biology. Lin YKielkopf CL (2008 May 20). X-ray structures of U2 snRNA-branchpoint duplexes containing conserved pseudouridines. Biochemistry. Kumar AOSwenson MCBenning MMKielkopf CL (2008 Mar 21). Structure of the central RNA recognition motif of human TIA-1 at 1.95A resolution. Biochemical and biophysical research communications. Swenson MCParanawithana SRMiller PSKielkopf CL (2007 Apr 17). Structure of a DNA repair substrate containing an alkyl interstrand cross-link at 1.65 A resolution. Biochemistry. Thickman KRSickmier EAKielkopf CL (2007 Feb 23). Alternative conformations at the RNA-binding surface of the N-terminal U2AF(65) RNA recognition motif. Journal of molecular biology. Sickmier EAFrato KEShen HParanawithana SRGreen MRKielkopf CL (2006 Jul 7). Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65. Molecular cell. Sickmier EAFrato KEKielkopf CL (2006 May 1). Crystallization and preliminary X-ray analysis of a U2AF65 variant in complex with a polypyrimidine-tract analogue by use of protein engineering. Acta crystallographica. Section F, Structural biology and crystallization communications. Thickman KRSwenson MCKabogo JMGryczynski ZKielkopf CL (2006 Feb 24). Multiple U2AF65 binding sites within SF3b155: thermodynamic and spectroscopic characterization of protein-protein interactions among pre-mRNA splicing factors. Journal of molecular biology. Manceau VSwenson MLe Caer JPSobel AKielkopf CLMaucuer A (2006 Feb). Major phosphorylation of SF1 on adjacent Ser-Pro motifs enhances interaction with U2AF65. The FEBS journal. Sickmier EABrekasis DParanawithana SBonanno JBPaget MSBurley SKKielkopf CL (2005 Jan). X-ray structure of a Rex-family repressor/NADH complex insights into the mechanism of redox sensing. Structure (London, England : 1993). Kielkopf CLLücke SGreen MR (2004 Jul 1). U2AF homology motifs: protein recognition in the RRM world. Genes & development. Kielkopf CLBurley SK (2002 Oct 1). X-ray structures of threonine aldolase complexes: structural basis of substrate recognition. Biochemistry. Kielkopf CLRodionova NAGreen MRBurley SK (2001 Sep 7). A novel peptide recognition mode revealed by the X-ray structure of a core U2AF35/U2AF65 heterodimer. Cell. Kielkopf CLDing SKuhn PRees DC (2000 Feb 25). Conformational flexibility of B-DNA at 0.74 A resolution: d(CCAGTACTGG)(2). Journal of molecular biology. Kielkopf CLErkkila KEHudson BPBarton JKRees DC (2000 Feb). Structure of a photoactive rhodium complex intercalated into DNA. Nature structural biology. Kielkopf CLBremer REWhite SSzewczyk JWTurner JMBaird EEDervan PBRees DC (2000 Jan 21). Structural effects of DNA sequence on T.A recognition by hydroxypyrrole/pyrrole pairs in the minor groove. Journal of molecular biology. Kielkopf CLWhite SSzewczyk JWTurner JMBaird EEDervan PBRees DC (1998 Oct 2). A structural basis for recognition of A.T and T.A base pairs in the minor groove of B-DNA. Science (New York, N.Y.). Kielkopf CLBaird EEDervan PBRees DC (1998 Feb). Structural basis for G.C recognition in the DNA minor groove. Nature structural biology.