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URMC / Labs / Korman Lab / SOPs
 

SOPs

Bleomycin-induced mouse model of scleroderma

Isolation and culture of scleroderma dermal fibroblasts

Assessments of collagen content: Western blot, hydroxyproline, and Sircol assays

Histologic assessment of pulmonary vascular disease

Quantitative PCR for fibrotic and adipogenic genes