Lab Protocols

Documents below can be downloaded from our shared folder on Box (secure)

RNA

• In vitro transcription
• Analytical urea PAGE
• C18 RNA purification
• Lyophilization
• Sep Pak desalting

Protein

• Electrophoresis stocks
• Protein PAGE recipes
• T7 prep (non-6His)

Molecular Biology

• Cell growth/transformation

Crystallization

• Joe’s Crystallization Stocks
• Joe’s 24-well Plate Tracker
• Torelli’s Home-Data-Set Form
• Jolanta’s DLS presentation

Methods and Reviews

• RNA Prep, Purif, Crystallization
• Metal Ion Coordination in RNA
• Torelli synchrotron-packing list
• SHELX RNA SAD Phasing
• RNA Preparation & Derivatives
• CNS Multi-conformational RNA

Ivan's Protocols

• 8HMBPMS2 Purification Protocol
• 6HTEV Protease Purification
• 6H-TEV-U1A Purification
• Co-crystallization of RNA Molecules with U1A
• CSB Media Recipe
• Detailed Western Blotting Protocol
• Expression in Ecoli using Autoinduction
• Frameshifting Protocol RRL
• Gluthathione Resin Regeneration
• GST-Prescission Protease Purification (GML)
• In vitro Transcription Recipe
• Native RNA Purification Protocol
• Ni-NTA Resin Regeneration
• Preparing Competent Cells
• Protocol for Harvesting Pfu (Barrick Lab)
• PyMOL Structure Preparation Notes
• Restriction Enzyme End Requirements (NEB)
• Superscript III RT Purification
• T7 RNAP Purification
• TEV Protease Cleavage Protocol
• VP-ITC Protocol

Andrew's Protocols

• PAGE gel for RNA
• Deprotection of synthetic RNA from Dharmacon
• Analytical Gel
• Post Urea-PAGE DEAE Purification of RNA
• SEC desalting purification of RNA