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Fees/ Billing

Nucleic Acid

Quality Assessment

**All samples will be assessed for concentration and quality.**
May include NanoDrop, Qubit, Agilent Bioanalyzer, and/or Agilent Tapestation (up to GRC discretion)

All sample types excluding Small RNA - $15/sample
Small RNA - $80/chip (up to 10 samples)

RNA Extraction/Purification

Trizol RNA Extraction - $34.00/sample*
Qiagen RNeasy Plus - $34.00/sample*
Dnase Treatment - $32.00/sample
* Does not Include quality assessment

DNA Extraction/Purification

Qiagen DNeasy Kit - $27.00/sample*
* Does not Include quality assessment

Sequencing

Next Generation Sequencing (HiSeq2500)

All pricing effective for projects submitted after July 1, 2017 and subject to change.  

Prices reflective of University of Rochester internal usage, external pricing is available. High-throughput batch pricing is available.  Contact us for more information.


Library Preparation 

Library Type

  Price/Sample  

TruSeq RNA v2 $250.00
TruSeq mRNA Stranded $250.00
TruSeq Total Stranded RNA
(Human, Mouse, Rat)
$350.00
TruSeq Small RNA $350.00
Low Input RNA-seq
(Clontech SMARTer Technology)
$315.00*
TruSeq DNA Nano  $284.00
TruSeq PCR-free $294.00
ChIP-seq** $280.00
NexteraXT $90.00
Agilent SureSelect whole exome $550.00
Whole Genome Bisulphite (WGBS) $384.00
16S Amplicon $40.00
ATAC-seq $102.00

* Includes SMART-seq amplification and Nextera library preparation.

**NGS library construction only.  We accept sheared/fragmented ChIP purified DNA only.

Note: All library prices listed include library QC, normalization, and pooling unless otherwise indicated.  Other libraries type are available upon request.  Contact us for additional information.

Single-Cell RNAseq (scRNAseq)

Fluidigm C1 - Contact us for pricing

10X Genomics - Contact us for pricing

FACS- based (SMART-seq2, SCRB-seq, etc...) - Contact us for pricing

Sequencing (HiSeq2500v4)

High Output Run (**Requires use of 8 lane flow cell**)

Lane output = ~250-280 million reads (clusters)

Single End Read (SER)

SER Cost per lane
1 x 50 $1,070.00
1 x 100 $1,392.00

 

Per End Read (PER)

PER Cost per lane
2 x 50 $2,005.00
2 x 125 $2,651.00

 

Rapid Run (**Requires use of 2 lane flow cell**)

Lane Output = ~150-180 million reads (clusters)

Single End Read (SER)

SER Cost per lane
1 x 50 $1,142.00
1 x 100 $1,440.00
1 x 150 $1,738.00
1 x 250 $2.036.00

 

Per End Read (PER)

PER Cost per lane
2 x 50 $2,095.00
2 x 75 $2,371.00
2 x 125 $2,691.00
2 x 150 $3,181.00
2 x 250 $3,903.00

***Users may request preference for High Output or Rapid Run flow cells, however, actual choice will be determined by GRC staff based on sequencing queue to ensure efficient operation of the sequencer.  Users will be billed for flow cell type utilized for their samples.

 

Sequencing (MiSeq)

PER Cost per chip
2 x 150 $2,100.00
2 x 250 $2,300.00
2 x 300 $2,900.00
Library Pool QC  $350.00

Contact us for information regarding run options for your given application  Turnaround time depends on sample queue at time of submission and is subject to change based on demand.

** Sequencing costs include basic data processing and analysis:

  • Demultiplexing
  • Read processing (filtering based on complexity and end trimming based on quality scores)
  • Mapping to reference genome using STAR or BWA
  • Differential expression analysis using DeSeq2

Additional analysis support can be provided. Contact us for more information.

***Additional fees may apply for analysis of data not generated by the GRC*** 

**Turnaround time is based on date of sample receipt.**

Quantitative PCR

Service Cost Quantity
ABI High Capacity Reverse Transcription $15.00 Sample
Nugen WT-Ovation PicoSL Kit (RNA Amplification) $86.00 Sample
QPCR Half Plate Setup 384
*up to 192 wells, includes no RT and no template controls, assays not included
$369.00 Half Plate
QPCR Full Plate Setup_384
*up to 384 wells, includes no RT and no template controls, assays not included
$574.00 Full Plate
QPCR Half Plate Setup_96
*up to 48 wells, includes no RT and no template controls, assays not included
$247.00 Half  Plate
QPCR Full Plate Setup_96
*up to 96 wells, includes no RT and no template controls, assays not included
$317.00 Full  Plate
ABI QuantStudio Instrument Run Fee $60.00 Plate
ABI Microfluidic Card (TLDA) Set-up And Run On ABI QuantStudio (Does not include TLDA) $80.00 TLDA

 

Micro Array Analysis

Service Cost Quantity
Processing Fee, preparation
with NuGEN (<100ng)
$400.00 Sample
+ $250 project fee
Processing Fee, preparation
with Ambion (>100ng)
$360.00 Sample
+ $250 project fee
Affymetrix 3' IVT Genechips
most common species
$400.00 Array
Affymetrix Gene 2.0 ST Genechips,
most common species
$175.00 Array