24-Hour Urine Collection
What is a 24-hour urine collection?
A 24-hour urine collection is a simple lab test that measures what’s in your urine.
The test is used to check kidney function. A 24-hour urine collection is done by collecting
your urine in a special container(s) over a full 24-hour period. The container(s)
must be kept cool until the urine is returned to the lab.
Urine is made up of water and dissolved chemicals, such as sodium and potassium. It
also contains urea. This is made when protein breaks down, and creatinine, which is
formed from muscle breakdown. Normally, urine contains certain amounts of these waste
products. If these amounts are not within a normal range or if other substances are
present, it may be a sign of a certain disease or condition.
Why might I need a 24-hour urine collection?
Twenty-four hour urine collection helps diagnose kidney problems. It is often done
to see how much creatinine clears through the kidneys. It’s also done to measure protein,
hormones, minerals, and other chemical compounds.
Conditions that can cause kidney disease include:
- Diabetic nephropathy. This happens when someone has uncontrolled diabetes. It causes high levels of protein
(albumin) in the urine and can lead to kidney damage.
- High blood pressure. Abnormally high blood pressure can lead to permanent kidney damage.
- Lupus nephritis. Lupus is an autoimmune disease where the immune system attacks the kidneys and damages
- Frequent urinary tract infections.
- Prolonged urinary tract blockage.
- Alport syndrome. This health problem causes vision and hearing problems, as well as progressive scarring
of the kidneys. The syndrome is passed down through families.
- Nephrotic syndrome. This health problem has several different causes. Symptoms include: protein in the
urine, low protein in the blood, high cholesterol levels, and tissue swelling.
- Polycystic kidney disease. This health problem causes the growth of many fluid-filled cysts in the kidneys. This
makes the kidneys larger and, over time, takes over and destroys working kidney tissue.
- Interstitial nephritis or pyelonephritis. This is an inflammation in the small structures in the kidney. It’s often caused by
- Screening for preeclampsia in pregnancy. Preeclampsia is a dangerous health problem that sometimes occurs in pregnancy. It
causes high blood pressure and can lead to organ failure.
There may be other reasons for your healthcare provider to recommend 24-hour urine
What are the risks of a 24-hour urine collection?
Twenty-four hour urine collection is a safe, easy test. People can collect urine on
Certain factors may affect the accuracy of a 24-hour urine collection. These factors
- Forgetting to collect some of your urine
- Going beyond the 24-hour collection period and collecting too much urine
- Losing urine from the specimen container through spilling
- Not keeping urine cold while collecting it
- Acute stress
- Vigorous exercise
- Certain foods, such as coffee, tea, cocoa, bananas, citrus fruits, and vanilla
There may be other risks depending on your specific medical problems. Be sure to discuss
any concerns with your healthcare provider before the collection.
How do I get ready for a 24-hour urine collection?
- Your healthcare provider will explain the procedure and you can ask questions.
- Make sure you understand if you need to avoid certain foods while collecting your
- You will be given large containers to store your urine and a container to urinate
into. Make sure you know how to use them. Have a cold place to store the urine while
you’re collecting it. For instance, a refrigerator or in a cooler on ice.
- You may be told to start the collection at a specific time.
- If possible, choose a 24-hour period when you will be at home so you do not have to
transport your urine.
- If you are pregnant or think you may be, tell your healthcare provider.
- Make sure your healthcare provider has a list of all medicines (prescription and over-the-counter),
herbs, vitamins, and supplements that you are taking.
Based on your medical condition, your healthcare provider may request other specific
What happens during a 24-hour urine collection?
A 24-hour urine collection may be done on an outpatient basis or during a hospital
stay. Procedures may vary depending on your condition and your healthcare provider's
Generally, a 24-hour urine collection follows this process:
- You will be given one or more containers for collecting and storing your urine. A
brown plastic container is typically used. A special pan that fits in the toilet or
a urinal may be used to collect the urine. You will need to transfer the urine from
the collecting container to the storage container. You will need to keep it cold.
- The 24-hour collection may start at any time during the day after you urinate, but
your healthcare provider may tell you when to start. It is common to start the collection
the first thing in the morning. It is important to collect all urine in the following
- Do not save the urine from your first time urinating. Flush this first specimen, but
note the time. This is the start time of the 24-hour collection.
- All urine, after the first flushed specimen, must be saved, stored, and kept cold.
This means keeping it either on ice or in a refrigerator for the next 24 hours.
- Try to urinate again at the same time, 24 hours after the start time, to finish the
collection process. If you can’t urinate at this time, it is OK.
- Once the urine collection has been completed, the urine containers need to be taken
to the lab as soon as possible. If you are doing the urine collection at home, you
will be given instructions on how and where to take it.
- Depending on your specific medical problem, you may be asked to repeat the collection
over several days.
What happens after a 24-hour urine collection?
There is no special type of care after a 24-hour urine collection. However, your healthcare
provider may give you other instructions after the procedure, depending on your particular
Before you agree to the test or the procedure make sure you know:
- The name of the test or procedure
- The reason you are having the test or procedure
- What results to expect and what they mean
- The risks and benefits of the test or procedure
- What the possible side effects or complications are
- When and where you are to have the test or procedure
- Who will do the test or procedure and what that person’s qualifications are
- What would happen if you did not have the test or procedure
- Any alternative tests or procedures to think about
- When and how will you get the results
- Who to call after the test or procedure if you have questions or problems
- How much will you have to pay for the test or procedure