URMC / Labs / Dumont Lab / Publications Recent Publications Clark KMJenkins JLFedoriw NDumont ME (2017 Feb). Human CaaX protease ZMPSTE24 expressed in yeast: Structure and inhibition by HIV protease inhibitors. Protein science : a publication of the Protein Society. Coudray NL Seyler SLasala RZhang ZClark KMDumont MERohou ABeckstein OStokes DL (2017 Jan). Structure of the SLC4 transporter Bor1p in an inward-facing conformation. Protein science : a publication of the Protein Society. Sridharan RConnelly SMNaider FDumont ME (2016 Sep 19). Variable Dependence of Signaling Output on Agonist Occupancy of Ste2p, a G Protein Coupled Receptor in Yeast. The Journal of biological chemistry. Coudray NLasala RZhang ZClark KMDumont MEStokes DL (2016 May 30). Deducing the Symmetry of Helical Assemblies: Applications to Membrane Proteins. Journal of structural biology. Zhu GKoszelak-Rosenblum MConnelly SMDumont MEMalkowski MG (2015 Dec 11). The Crystal Structure of an Integral Membrane Fatty Acid α-Hydroxylase. The Journal of biological chemistry. Zuber JDanial SAConnelly SMNaider FDumont ME (2015 Mar 10). Identification of destabilizing and stabilizing mutations of Ste2p, a G protein-coupled receptor in Saccharomyces cerevisiae. Biochemistry. Mathew EDumont ME (2015). A Novel Screening Approach for Optimal and Functional Fusion of T4 Lysozyme in GPCRs. Methods in enzymology. Sridharan RZuber JConnelly SMMathew EDumont ME (2014 Jan). Fluorescent approaches for understanding interactions of ligands with G protein coupled receptors. Biochimica et biophysica acta. Cohen LSArshava BKauffman SMathew EFracchiolla KEDing FXDumont MEBecker JMNaider F (2014 Jan). Guided reconstitution of membrane protein fragments. Biopolymers. Pryor EEHoranyi PSClark KMFedoriw NConnelly SMKoszelak-Rosenblum MZhu GMalkowski MGWiener MCDumont ME (2013 Mar 29). Structure of the integral membrane protein CAAX protease Ste24p. Science (New York, N.Y.). Pieper USchlessinger AKloppmann EChang GAChou JJDumont MEFox BGFromme PHendrickson WAMalkowski MGRees DCStokes DLStowell MHWiener MCRost BStroud RMStevens RCSali A (2013 Feb). Coordinating the impact of structural genomics on the human α-helical transmembrane proteome. Nature structural & molecular biology. Mathew EDing FXNaider FDumont ME (2013 Jan). Functional fusions of T4 lysozyme in the third intracellular loop of a G protein-coupled receptor identified by a random screening approach in yeast. Protein engineering, design & selection : PEDS. Gehret AUConnelly SMDumont ME (2012 Oct). Functional and physical interactions among Saccharomyces cerevisiae α-factor receptors. Eukaryotic cell. Lesoine JFVenkataraman PAMaloney PCDumont MENovotny L (2012 Jun 13). Nanochannel-based single molecule recycling. Nano letters. Taslimi AMathew ECelić AWessel SDumont ME (2012 May 18). Identifying functionally important conformational changes in proteins: activation of the yeast α-factor receptor Ste2p. Journal of molecular biology. Kang DCVenkataraman PADumont MEMaloney PC (2011 Oct 4). Oligomeric state of the oxalate transporter, OxlT. Biochemistry. Mathew EBajaj AConnelly SMSargsyan HDing FXHajduczok AGNaider FDumont ME (2011 Jun 17). Differential interactions of fluorescent agonists and antagonists with the yeast G protein coupled receptor Ste2p. Journal of molecular biology. Tantry SDing FXDumont MBecker JMNaider F (2010 Jun 22). Binding of fluorinated phenylalanine alpha-factor analogues to Ste2p: evidence for a cation-pi binding interaction between a peptide ligand and its cognate G protein-coupled receptor. Biochemistry. Clark KMFedoriw NRobinson KConnelly SMRandles JMalkowski MGDeTitta GTDumont ME (2010 Jun). Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography. Protein expression and purification. Shi SNotenboom SDumont MEBallatori N (2010). Identification of human gene products containing Pro-Pro-x-Tyr (PY) motifs that enhance glutathione and endocytotic marker uptake in yeast. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. Chloupková MPickert ALee JYSouza STrinh YTConnelly SMDumont MEDean MUrbatsch IL (2007 Jul 10). Expression of 25 human ABC transporters in the yeast Pichia pastoris and characterization of the purified ABCC3 ATPase activity. Biochemistry. Bajaj AConnelly SMGehret AUNaider FDumont ME (2007 Jun). Role of extracellular charged amino acids in the yeast alpha-factor receptor. Biochimica et biophysica acta. White MAClark KMGrayhack EJDumont ME (2007 Jan 19). Characteristics affecting expression and solubilization of yeast membrane proteins. Journal of molecular biology. Wang ZStalcup LDHarvey BJWeber JChloupkova MDumont MEDean MUrbatsch IL (2006 Aug 15). Purification and ATP hydrolysis of the putative cholesterol transporters ABCG5 and ABCG8. Biochemistry. Gehret AUBajaj ANaider FDumont ME (2006 Jul 28). Oligomerization of the yeast alpha-factor receptor: implications for dominant negative effects of mutant receptors. The Journal of biological chemistry. Gelperin DMWhite MAWilkinson MLKon YKung LAWise KJLopez-Hoyo NJiang LPiccirillo SYu HGerstein MDumont MEPhizicky EMSnyder MGrayhack EJ (2005 Dec 1). Biochemical and genetic analysis of the yeast proteome with a movable ORF collection. Genes & development. Bajaj ACelić ADing FXNaider FBecker JMDumont ME (2004 Oct 26). A fluorescent alpha-factor analogue exhibits multiple steps on binding to its G protein coupled receptor in yeast. Biochemistry. Alexandrov AVignali MLaCount DJQuartley Ede Vries CDe Rosa DBabulski JMitchell SFSchoenfeld LWFields SHol WGDumont MEPhizicky EMGrayhack EJ (2004 Sep). A facile method for high-throughput co-expression of protein pairs. Molecular & cellular proteomics : MCP. Roginskaya MConnelly SMKim KSPatel DDumont ME (2004 Mar). Effects of mutations in the N terminal region of the yeast G protein alpha-subunit Gpa1p on signaling by pheromone receptors. Molecular genetics and genomics : MGG. Celić AConnelly SMMartin NPDumont ME (2004). Intensive mutational analysis of G protein-coupled receptors in yeast. Methods in molecular biology (Clifton, N.J.). Celić AMartin NPSon CDBecker JMNaider FDumont ME (2003 Mar 18). Sequences in the intracellular loops of the yeast pheromone receptor Ste2p required for G protein activation. Biochemistry. Connelly SMYuan JDing FXNaider FBecker JMDumont ME (2003 Jan 01). Effects of Inserting Intracellular Loops of the Yeast a-factor Receptor, Ste3p, into the a-factor Receptor, Ste2p Yeast. Arevalo EEstephan RMadeo JArshava BDumont MEBecker JMNaider F (2003 Jan 01). Biosynthesis and Biophysical Analysis of Domains of a Yeast G protein-coupled receptor Biopolymers (Peptide Science). Martin NPCelić ADumont ME (2002 Apr 12). Mutagenic mapping of helical structures in the transmembrane segments of the yeast alpha-factor receptor. Journal of molecular biology. Erlenbach IKostenis ESchmidt CSerradeil-Le Gal CRaufaste DDumont MEPausch MHWess J (2001 Aug 3). Single amino acid substitutions and deletions that alter the G protein coupling properties of the V2 vasopressin receptor identified in yeast by receptor random mutagenesis. The Journal of biological chemistry. Sommers CMMartin NPAkal-Strader ABecker JMNaider FDumont ME (2000 Jun 13). A limited spectrum of mutations causes constitutive activation of the yeast alpha-factor receptor. Biochemistry. Leavitt LMMacaluso CRKim KSMartin NPDumont ME (1999 Jul). Dominant negative mutations in the alpha-factor receptor, a G protein-coupled receptor encoded by the STE2 gene of the yeast Saccharomyces cerevisiae. Molecular & general genetics : MGG. Martin NPLeavitt LMSommers CMDumont ME (1999 Jan 12). Assembly of G protein-coupled receptors from fragments: identification of functional receptors with discontinuities in each of the loops connecting transmembrane segments. Biochemistry. Sommers CMDumont ME (1999 Jan 01). Genetic Approaches for Studying the Structure and Function of G Protein-Coupled receptors in Yeast in Structure-Function Analysis of G Protein-Coupled Receptors ( J. Wess, ed.) Vol. III of Receptor Biochemistry and Methodology, , John Wiley and Sons, New York. Rebbeor JFConnolly GCDumont MEBallatori N (1998 Dec 11). ATP-dependent transport of reduced glutathione on YCF1, the yeast orthologue of mammalian multidrug resistance associated proteins. The Journal of biological chemistry. Rebbeor JFConnolly GCDumont MEBallatori N (1998 Sep 15). ATP-dependent transport of reduced glutathione in yeast secretory vesicles. The Biochemical journal. Pollock WBRosell FITwitchett MBDumont MEMauk AG (1998 Apr 28). Bacterial expression of a mitochondrial cytochrome c. Trimethylation of lys72 in yeast iso-1-cytochrome c and the alkaline conformational transition. Biochemistry. Sommers CMDumont ME (1997 Feb 28). Genetic interactions among the transmembrane segments of the G protein coupled receptor encoded by the yeast STE2 gene. Journal of molecular biology. Wang XDumont MESherman F (1996 Mar 22). Sequence requirements for mitochondrial import of yeast cytochrome c. The Journal of biological chemistry. Dumont MECorin AFCampbell GA (1994 Jun 14). Noncovalent binding of heme induces a compact apocytochrome c structure. Biochemistry. Dumont MESchlichter JBCardillo TSHayes MKBethlendy GSherman F (1993 Oct). CYC2 encodes a factor involved in mitochondrial import of yeast cytochrome c. Molecular and cellular biology. Dumont ME (1993 Jan 01). Mitochondrial Import of Cytochrome c. in: Protein Synthesis and Targeting in Yeast (A. Brown J. McCarthy, F. Sherman, and M. Tuite, ed.s) Springer-Verlag, N.Y.. Sherman FMoerschell RPTsunasawa SSternglanz RDumont ME (1993 Jan 01). Co- and Post-Translational Processes and Mitochondrial Import of Yeast Cytochrome c. Translational Regulation of Gene Expression II (J. Ilan, ed.)Plenum Publishing, N.Y.. Dumont MECardillo TSHayes MKSherman F (1991 Nov). Role of cytochrome c heme lyase in mitochondrial import and accumulation of cytochrome c in Saccharomyces cerevisiae. Molecular and cellular biology. Dumont MDMathews AJNall BTBaim SBEustice DCSherman F (1990 Feb 15). Differential stability of two apo-isocytochromes c in the yeast Saccharomyces cerevisiae. The Journal of biological chemistry.