Conventional histology is based on light microscopy of thin tissue sections stained with absorptive dyes. These techniques, developed more than 100 years ago, are the standard for the evaluation of nearly all cancer. However, they are slow, costly, subjective and inherently analog because tissue most be fixed in formaldehyde and then cut into thin wax sections for viewing.
Virtual transillumination rendering of a two photon image of skin cancer. On the left the true color image is shown, on the right, the image is rendered to resemble a conventional histology slide.