URMC / Labs / Grayhack Lab / Publications

Recent Publications

1. Wakabayashi H
2. Zhu M
3. Grayhack EJ
4. Mathews DH
5. Ermolenko DN
40S ribosomal subunits scan mRNA for the start codon by one-dimensional diffusion.; bioRxiv : the preprint server for biology. 2025 Jan 04.
1. Houston L
2. Platten EM
3. Connelly SM
4. Wang J
5. Grayhack EJ
Frameshifting at collided ribosomes is modulated by elongation factor eEF3 and by Integrated Stress Response regulators Gcn1 and Gcn20.; RNA (New York, N.Y.). 2021 Dec 16.
1. Ghoneim DH
2. Zhang X
3. Brule CE
4. Mathews DH
5. Grayhack EJ
Conservation of location of several specific inhibitory codon pairs in the Saccharomyces sensu stricto yeasts reveals translational selection.; Nucleic acids research. 2018 Dec 21.
1. Wang J
2. Zhou J
3. Yang Q
4. Grayhack EJ
Multi-protein Bridging Factor 1(Mbf1), Rps3 and Asc1 prevent stalled ribosomes from frameshifting.; eLife; Vol 7. 2018 Nov 22.
1. Coller J
2. Grayhack EJ
The analysis of translational mechanisms that modulate gene expression.; Methods (San Diego, Calif.); Vol 137. 2018 Mar 15.
1. Brule CE
2. Grayhack EJ
Synonymous Codons: Choose Wisely for Expression.; Trends in genetics : TIG. 2017 Mar 11.
1. Patra B
2. Kon Y
3. Yadav G
4. Sevold AW
5. Frumkin JP
6. Vallabhajosyula RR
7. Hintze A
8. Østman B
9. Schossau J
10. Bhan A
11. Marzolf B
12. Tamashiro JK
13. Kaur A
14. Baliga NS
15. Grayhack EJ
16. Adami C
17. Galas DJ
18. Raval A
19. Phizicky EM
20. Ray A
A genome wide dosage suppressor network reveals genomic robustness.; Nucleic acids research; Vol 45(1). 2017 Jan 09.
1. Gamble CE
2. Brule CE
3. Dean KM
4. Fields S
5. Grayhack EJ
Adjacent Codons Act in Concert to Modulate Translation Efficiency in Yeast.; Cell. 2016 Jun 29.
1. Brule CE
2. Dean KM
3. Grayhack EJ
RNA-ID, a Powerful Tool for Identifying and Characterizing Regulatory Sequences.; Methods in enzymology; Vol 572. 2016.
1. Quivey RG
2. Grayhack EJ
3. Faustoferri RC
4. Hubbard CJ
5. Baldeck JD
6. Wolf AS
7. MacGilvray ME
8. Rosalen PL
9. Scott-Anne K
10. Santiago B
11. Gopal S
12. Payne J
13. Marquis RE
Functional profiling in Streptococcus mutans: construction and examination of a genomic collection of gene deletion mutants.; Molecular oral microbiology; Vol 30(6). 2015 Dec.
1. Wolf AS
2. Grayhack EJ
Asc1, homolog of human RACK1, prevents frameshifting in yeast by ribosomes stalled at CGA codon repeats.; RNA (New York, N.Y.); Vol 21(5). 2015 May.
1. Guy MP
2. Young DL
3. Payea MJ
4. Zhang X
5. Kon Y
6. Dean KM
7. Grayhack EJ
8. Mathews DH
9. Fields S
10. Phizicky EM
Identification of the determinants of tRNA function and susceptibility to rapid tRNA decay by high-throughput in vivo analysis.; Genes & development; Vol 28(15). 2014 Aug 1.
1. Letzring DP
2. Wolf AS
3. Brule CE
4. Grayhack EJ
Translation of CGA codon repeats in yeast involves quality control components and ribosomal protein L1.; RNA (New York, N.Y.); Vol 19(9). 2013 Sep.
1. Grant TD
2. Luft JR
3. Wolfley JR
4. Snell ME
5. Tsuruta H
6. Corretore S
7. Quartley E
8. Phizicky EM
9. Grayhack EJ
10. Snell EH
The structure of yeast glutaminyl-tRNA synthetase and modeling of its interaction with tRNA.; Journal of molecular biology; Vol 425(14). 2013 Jul 24.
1. Dean KM
2. Grayhack EJ
RNA-ID, a highly sensitive and robust method to identify cis-regulatory sequences using superfolder GFP and a fluorescence-based assay.; RNA (New York, N.Y.); Vol 18(12). 2012 Dec.
1. Grant TD
2. Snell EH
3. Luft JR
4. Quartley E
5. Corretore S
6. Wolfley JR
7. Snell ME
8. Hadd A
9. Perona JJ
10. Phizicky EM
11. Grayhack EJ
Structural conservation of an ancient tRNA sensor in eukaryotic glutaminyl-tRNA synthetase.; Nucleic acids research; Vol 40(8). 2012 Apr.
1. Letzring DP
2. Dean KM
3. Grayhack EJ
Control of translation efficiency in yeast by codon-anticodon interactions.; RNA (New York, N.Y.); Vol 16(12). 2010 Dec.
1. Quartley E
2. Alexandrov A
3. Mikucki M
4. Buckner FS
5. Hol WG
6. DeTitta GT
7. Phizicky EM
8. Grayhack EJ
Heterologous expression of L. major proteins in S. cerevisiae: a test of solubility, purity, and gene recoding.; Journal of structural and functional genomics; Vol 10(3). 2009 Sep.
1. Chernyakov I
2. Whipple JM
3. Kotelawala L
4. Grayhack EJ
5. Phizicky EM
Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1.; Genes & development; Vol 22(10). 2008 May 15.
1. Kotelawala L
2. Grayhack EJ
3. Phizicky EM
Identification of yeast tRNA Um(44) 2'-O-methyltransferase (Trm44) and demonstration of a Trm44 role in sustaining levels of specific tRNA(Ser) species.; RNA (New York, N.Y.); Vol 14(1). 2008 Jan.
1. Jackman JE
2. Grayhack EJ
3. Phizicky EM
The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins.; Methods in molecular biology (Clifton, N.J.); Vol 488. 2008.
1. Chernyakov I
2. Baker MA
3. Grayhack EJ
4. Phizicky EM
Chapter 11. Identification and analysis of tRNAs that are degraded in Saccharomyces cerevisiae due to lack of modifications.; Methods in enzymology; Vol 449. 2008.
1. Malkowski MG
2. Quartley E
3. Friedman AE
4. Babulski J
5. Kon Y
6. Wolfley J
7. Said M
8. Luft JR
9. Phizicky EM
10. DeTitta GT
11. Grayhack EJ
Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing.; Proceedings of the National Academy of Sciences of the United States of America; Vol 104(16). 2007 Apr 17.
1. Wilkinson ML
2. Crary SM
3. Jackman JE
4. Grayhack EJ
5. Phizicky EM
The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4.; RNA (New York, N.Y.); Vol 13(3). 2007 Mar.
1. White MA
2. Clark KM
3. Grayhack EJ
4. Dumont ME
Characteristics affecting expression and solubilization of yeast membrane proteins.; Journal of molecular biology; Vol 365(3). 2007 Jan 19.
1. Jackman JE
2. Kotelawala L
3. Grayhack EJ
4. Phizicky EM
Identification and characterization of modification enzymes by biochemical analysis of the proteome.; Methods in enzymology; Vol 425. 2007.
1. Phizicky EM
2. Grayhack EJ
Proteome-scale analysis of biochemical activity.; Critical reviews in biochemistry and molecular biology; Vol 41(5). 2006 Sep.
1. Alexandrov A
2. Chernyakov I
3. Gu W
4. Hiley SL
5. Hughes TR
6. Grayhack EJ
7. Phizicky EM
Rapid tRNA decay can result from lack of nonessential modifications.; Molecular cell; Vol 21(1). 2006 Jan 6.
1. Gelperin DM
2. White MA
3. Wilkinson ML
4. Kon Y
5. Kung LA
6. Wise KJ
7. Lopez-Hoyo N
8. Jiang L
9. Piccirillo S
10. Yu H
11. Gerstein M
12. Dumont ME
13. Phizicky EM
14. Snyder M
15. Grayhack EJ
Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.; Genes & development; Vol 19(23). 2005 Dec 1.
1. Gu W
2. Hurto RL
3. Hopper AK
4. Grayhack EJ
5. Phizicky EM
Depletion of Saccharomyces cerevisiae tRNA(His) guanylyltransferase Thg1p leads to uncharged tRNAHis with additional m(5)C.; Molecular and cellular biology; Vol 25(18). 2005 Sep.
1. Ma X
2. Yang C
3. Alexandrov A
4. Grayhack EJ
5. Behm-Ansmant I
6. Yu YT
Pseudouridylation of yeast U2 snRNA is catalyzed by either an RNA-guided or RNA-independent mechanism.; The EMBO journal; Vol 24(13). 2005 Jul 6.
1. Alexandrov A
2. Grayhack EJ
3. Phizicky EM
tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p.; RNA (New York, N.Y.); Vol 11(5). 2005 May.
1. Alexandrov A
2. Vignali M
3. LaCount DJ
4. Quartley E
5. de Vries C
6. De Rosa D
7. Babulski J
8. Mitchell SF
9. Schoenfeld LW
10. Fields S
11. Hol WG
12. Dumont ME
13. Phizicky EM
14. Grayhack EJ
A facile method for high-throughput co-expression of protein pairs.; Molecular & cellular proteomics : MCP; Vol 3(9). 2004 Sep.
1. Van Slyke C
2. Grayhack EJ
The essential transcription factor Reb1p interacts with the CLB2 UAS outside of the G2/M control region.; Nucleic acids research; Vol 31(15). 2003 Aug 1.
1. Phizicky EM
2. Martzen MR
3. McCraith SM
4. Spinelli SL
5. Xing F
6. Shull NP
7. Van Slyke C
8. Montagne RK
9. Torres FM
10. Fields S
11. Grayhack EJ
Biochemical genomics approach to map activities to genes.; Methods in enzymology; Vol 350. 2002.
1. Grayhack EJ
2. Phizicky EM
Genomic analysis of biochemical function.; Current opinion in chemical biology; Vol 5(1). 2001 Feb.
1. Martzen MR
2. McCraith SM
3. Spinelli SL
4. Torres FM
5. Fields S
6. Grayhack EJ
7. Phizicky EM
A biochemical genomics approach for identifying genes by the activity of their products.; Science (New York, N.Y.); Vol 286(5442). 1999 Nov 5.
1. Kuo MH
2. Nadeau ET
3. Grayhack EJ
Multiple phosphorylated forms of the Saccharomyces cerevisiae Mcm1 protein include an isoform induced in response to high salt concentrations.; Molecular and cellular biology; Vol 17(2). 1997 Feb.
1. Kuo MH
2. Grayhack E
A library of yeast genomic MCM1 binding sites contains genes involved in cell cycle control, cell wall and membrane structure, and metabolism.; Molecular and cellular biology; Vol 14(1). 1994 Jan.
1. Grayhack EJ
The yeast alpha 1 and MCM1 proteins bind a single strand of their duplex DNA recognition site.; Molecular and cellular biology; Vol 12(8). 1992 Aug.
1. Yang XJ
2. Hart CM
3. Grayhack EJ
4. Roberts JW
Transcription antitermination by phage lambda gene Q protein requires a DNA segment spanning the RNA start site.; Genes & development; Vol 1(3). 1987 May.
1. Grayhack EJ
2. Yang XJ
3. Lau LF
4. Roberts JW
Phage lambda gene Q antiterminator recognizes RNA polymerase near the promoter and accelerates it through a pause site.; Cell; Vol 42(1). 1985 Aug.
1. Grayhack EJ
2. Roberts JW
Purification of the bacteriophage lambda late gene regulator encoded by gene Q.; The Journal of biological chemistry; Vol 258(15). 1983 Aug 10.
1. Grayhack EJ
2. Roberts JW
The phage lambda Q gene product: activity of a transcription antiterminator in vitro.; Cell; Vol 30(2). 1982 Sep.