Recent Publications

• 1. Houston L
  2. Platten EM
  3. Connelly SM
  4. Wang J
  5. Grayhack EJ
  Frameshifting at collided ribosomes is modulated by elongation factor eEF3 and by Integrated Stress Response regulators Gcn1 and Gcn20.; RNA (New York, N.Y.). 2021 Dec 16.
• 1. Ghoneim DH
  2. Zhang X
  3. Brule CE
  4. Mathews DH
  5. Grayhack EJ
  Conservation of location of several specific inhibitory codon pairs in the Saccharomyces sensu stricto yeasts reveals translational selection.; Nucleic acids research. 2018 Dec 21.
• 1. Wang J
  2. Zhou J
  3. Yang Q
  4. Grayhack EJ
  Multi-protein Bridging Factor 1(Mbf1), Rps3 and Asc1 prevent stalled ribosomes from frameshifting.; eLife; Vol 7. 2018 Nov 22.
• 1. Coller J
  2. Grayhack EJ
  The analysis of translational mechanisms that modulate gene expression.; Methods (San Diego, Calif.); Vol 137. 2018 Mar 15.
• 1. Brule CE
  2. Grayhack EJ
  Synonymous Codons: Choose Wisely for Expression.; Trends in genetics : TIG. 2017 Mar 11.
• 1. Patra B
  2. Kon Y
  3. Yadav G
  4. Sevold AW
  5. Frumkin JP
  6. Vallabhajosyula RR
  7. Hintze A
  8. Østman B
  9. Schossau J
  10. Bhan A
  11. Marzolf B
  12. Tamashiro JK
  13. Kaur A
  14. Baliga NS
  15. Grayhack EJ
  16. Adami C
  17. Galas DJ
  18. Raval A
  19. Phizicky EM
  20. Ray A
  A genome wide dosage suppressor network reveals genomic robustness.; Nucleic acids research; Vol 45(1). 2017 Jan 09.
• 1. Gamble CE
  2. Brule CE
  3. Dean KM
  4. Fields S
  5. Grayhack EJ
  Adjacent Codons Act in Concert to Modulate Translation Efficiency in Yeast.; Cell. 2016 Jun 29.
• 1. Brule CE
  2. Dean KM
  3. Grayhack EJ
  RNA-ID, a Powerful Tool for Identifying and Characterizing Regulatory Sequences.; Methods in enzymology; Vol 572. 2016.
• 1. Quivey RG
  2. Grayhack EJ
  3. Faustoferri RC
  4. Hubbard CJ
  5. Baldeck JD
  6. Wolf AS
  7. MacGilvray ME
  8. Rosalen PL
  9. Scott-Anne K
  10. Santiago B
  11. Gopal S
  12. Payne J
  13. Marquis RE
  Functional profiling in Streptococcus mutans: construction and examination of a genomic collection of gene deletion mutants.; Molecular oral microbiology; Vol 30(6). 2015 Dec.
• 1. Wolf AS
  2. Grayhack EJ
  Asc1, homolog of human RACK1, prevents frameshifting in yeast by ribosomes stalled at CGA codon repeats.; RNA (New York, N.Y.); Vol 21(5). 2015 May.
• 1. Guy MP
  2. Young DL
  3. Payea MJ
  4. Zhang X
  5. Kon Y
  6. Dean KM
  7. Grayhack EJ
  8. Mathews DH
  9. Fields S
  10. Phizicky EM
  Identification of the determinants of tRNA function and susceptibility to rapid tRNA decay by high-throughput in vivo analysis.; Genes & development; Vol 28(15). 2014 Aug 1.
• 1. Letzring DP
  2. Wolf AS
  3. Brule CE
  4. Grayhack EJ
  Translation of CGA codon repeats in yeast involves quality control components and ribosomal protein L1.; RNA (New York, N.Y.); Vol 19(9). 2013 Sep.
• 1. Grant TD
  2. Luft JR
  3. Wolfley JR
  4. Snell ME
  5. Tsuruta H
  6. Corretore S
  7. Quartley E
  8. Phizicky EM
  9. Grayhack EJ
  10. Snell EH
  The structure of yeast glutaminyl-tRNA synthetase and modeling of its interaction with tRNA.; Journal of molecular biology; Vol 425(14). 2013 Jul 24.
• 1. Dean KM
  2. Grayhack EJ
  RNA-ID, a highly sensitive and robust method to identify cis-regulatory sequences using superfolder GFP and a fluorescence-based assay.; RNA (New York, N.Y.); Vol 18(12). 2012 Dec.
• 1. Grant TD
  2. Snell EH
  3. Luft JR
  4. Quartley E
  5. Corretore S
  6. Wolfley JR
  7. Snell ME
  8. Hadd A
  9. Perona JJ
  10. Phizicky EM
  11. Grayhack EJ
  Structural conservation of an ancient tRNA sensor in eukaryotic glutaminyl-tRNA synthetase.; Nucleic acids research; Vol 40(8). 2012 Apr.
• 1. Letzring DP
  2. Dean KM
  3. Grayhack EJ
  Control of translation efficiency in yeast by codon-anticodon interactions.; RNA (New York, N.Y.); Vol 16(12). 2010 Dec.
• 1. Quartley E
  2. Alexandrov A
  3. Mikucki M
  4. Buckner FS
  5. Hol WG
  6. DeTitta GT
  7. Phizicky EM
  8. Grayhack EJ
  Heterologous expression of L. major proteins in S. cerevisiae: a test of solubility, purity, and gene recoding.; Journal of structural and functional genomics; Vol 10(3). 2009 Sep.
• 1. Chernyakov I
  2. Whipple JM
  3. Kotelawala L
  4. Grayhack EJ
  5. Phizicky EM
  Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1.; Genes & development; Vol 22(10). 2008 May 15.
• 1. Kotelawala L
  2. Grayhack EJ
  3. Phizicky EM
  Identification of yeast tRNA Um(44) 2'-O-methyltransferase (Trm44) and demonstration of a Trm44 role in sustaining levels of specific tRNA(Ser) species.; RNA (New York, N.Y.); Vol 14(1). 2008 Jan.
• 1. Jackman JE
  2. Grayhack EJ
  3. Phizicky EM
  The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins.; Methods in molecular biology (Clifton, N.J.); Vol 488. 2008.
• 1. Chernyakov I
  2. Baker MA
  3. Grayhack EJ
  4. Phizicky EM
  Chapter 11. Identification and analysis of tRNAs that are degraded in Saccharomyces cerevisiae due to lack of modifications.; Methods in enzymology; Vol 449. 2008.
• 1. Malkowski MG
  2. Quartley E
  3. Friedman AE
  4. Babulski J
  5. Kon Y
  6. Wolfley J
  7. Said M
  8. Luft JR
  9. Phizicky EM
  10. DeTitta GT
  11. Grayhack EJ
  Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing.; Proceedings of the National Academy of Sciences of the United States of America; Vol 104(16). 2007 Apr 17.
• 1. Wilkinson ML
  2. Crary SM
  3. Jackman JE
  4. Grayhack EJ
  5. Phizicky EM
  The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4.; RNA (New York, N.Y.); Vol 13(3). 2007 Mar.
• 1. White MA
  2. Clark KM
  3. Grayhack EJ
  4. Dumont ME
  Characteristics affecting expression and solubilization of yeast membrane proteins.; Journal of molecular biology; Vol 365(3). 2007 Jan 19.
• 1. Jackman JE
  2. Kotelawala L
  3. Grayhack EJ
  4. Phizicky EM
  Identification and characterization of modification enzymes by biochemical analysis of the proteome.; Methods in enzymology; Vol 425. 2007.
• 1. Phizicky EM
  2. Grayhack EJ
  Proteome-scale analysis of biochemical activity.; Critical reviews in biochemistry and molecular biology; Vol 41(5). 2006 Sep.
• 1. Alexandrov A
  2. Chernyakov I
  3. Gu W
  4. Hiley SL
  5. Hughes TR
  6. Grayhack EJ
  7. Phizicky EM
  Rapid tRNA decay can result from lack of nonessential modifications.; Molecular cell; Vol 21(1). 2006 Jan 6.
• 1. Gelperin DM
  2. White MA
  3. Wilkinson ML
  4. Kon Y
  5. Kung LA
  6. Wise KJ
  7. Lopez-Hoyo N
  8. Jiang L
  9. Piccirillo S
  10. Yu H
  11. Gerstein M
  12. Dumont ME
  13. Phizicky EM
  14. Snyder M
  15. Grayhack EJ
  Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.; Genes & development; Vol 19(23). 2005 Dec 1.
• 1. Gu W
  2. Hurto RL
  3. Hopper AK
  4. Grayhack EJ
  5. Phizicky EM
  Depletion of Saccharomyces cerevisiae tRNA(His) guanylyltransferase Thg1p leads to uncharged tRNAHis with additional m(5)C.; Molecular and cellular biology; Vol 25(18). 2005 Sep.
• 1. Ma X
  2. Yang C
  3. Alexandrov A
  4. Grayhack EJ
  5. Behm-Ansmant I
  6. Yu YT
  Pseudouridylation of yeast U2 snRNA is catalyzed by either an RNA-guided or RNA-independent mechanism.; The EMBO journal; Vol 24(13). 2005 Jul 6.
• 1. Alexandrov A
  2. Grayhack EJ
  3. Phizicky EM
  tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p.; RNA (New York, N.Y.); Vol 11(5). 2005 May.
• 1. Alexandrov A
  2. Vignali M
  3. LaCount DJ
  4. Quartley E
  5. de Vries C
  6. De Rosa D
  7. Babulski J
  8. Mitchell SF
  9. Schoenfeld LW
  10. Fields S
  11. Hol WG
  12. Dumont ME
  13. Phizicky EM
  14. Grayhack EJ
  A facile method for high-throughput co-expression of protein pairs.; Molecular & cellular proteomics : MCP; Vol 3(9). 2004 Sep.
• 1. Van Slyke C
  2. Grayhack EJ
  The essential transcription factor Reb1p interacts with the CLB2 UAS outside of the G2/M control region.; Nucleic acids research; Vol 31(15). 2003 Aug 1.
• 1. Phizicky EM
  2. Martzen MR
  3. McCraith SM
  4. Spinelli SL
  5. Xing F
  6. Shull NP
  7. Van Slyke C
  8. Montagne RK
  9. Torres FM
  10. Fields S
  11. Grayhack EJ
  Biochemical genomics approach to map activities to genes.; Methods in enzymology; Vol 350. 2002.
• 1. Grayhack EJ
  2. Phizicky EM
  Genomic analysis of biochemical function.; Current opinion in chemical biology; Vol 5(1). 2001 Feb.
• 1. Martzen MR
  2. McCraith SM
  3. Spinelli SL
  4. Torres FM
  5. Fields S
  6. Grayhack EJ
  7. Phizicky EM
  A biochemical genomics approach for identifying genes by the activity of their products.; Science (New York, N.Y.); Vol 286(5442). 1999 Nov 5.
• 1. Kuo MH
  2. Nadeau ET
  3. Grayhack EJ
  Multiple phosphorylated forms of the Saccharomyces cerevisiae Mcm1 protein include an isoform induced in response to high salt concentrations.; Molecular and cellular biology; Vol 17(2). 1997 Feb.
• 1. Kuo MH
  2. Grayhack E
  A library of yeast genomic MCM1 binding sites contains genes involved in cell cycle control, cell wall and membrane structure, and metabolism.; Molecular and cellular biology; Vol 14(1). 1994 Jan.
• 1. Grayhack EJ
  The yeast alpha 1 and MCM1 proteins bind a single strand of their duplex DNA recognition site.; Molecular and cellular biology; Vol 12(8). 1992 Aug.
• 1. Yang XJ
  2. Hart CM
  3. Grayhack EJ
  4. Roberts JW
  Transcription antitermination by phage lambda gene Q protein requires a DNA segment spanning the RNA start site.; Genes & development; Vol 1(3). 1987 May.
• 1. Grayhack EJ
  2. Yang XJ
  3. Lau LF
  4. Roberts JW
  Phage lambda gene Q antiterminator recognizes RNA polymerase near the promoter and accelerates it through a pause site.; Cell; Vol 42(1). 1985 Aug.
• 1. Grayhack EJ
  2. Roberts JW
  Purification of the bacteriophage lambda late gene regulator encoded by gene Q.; The Journal of biological chemistry; Vol 258(15). 1983 Aug 10.
• 1. Grayhack EJ
  2. Roberts JW
  The phage lambda Q gene product: activity of a transcription antiterminator in vitro.; Cell; Vol 30(2). 1982 Sep.