URMC / Labs / Grayhack Lab / Publications Recent Publications Ghoneim DHZhang XBrule CEMathews DHGrayhack EJ Conservation of location of several specific inhibitory codon pairs in the Saccharomyces sensu stricto yeasts reveals translational selection.; Nucleic acids research. 2018 Dec 21. Wang JZhou JYang QGrayhack EJ Multi-protein Bridging Factor 1(Mbf1), Rps3 and Asc1 prevent stalled ribosomes from frameshifting.; eLife; Vol 7. 2018 Nov 22. Coller JGrayhack EJ The analysis of translational mechanisms that modulate gene expression.; Methods (San Diego, Calif.); Vol 137. 2018 Mar 15. Brule CEGrayhack EJ Synonymous Codons: Choose Wisely for Expression.; Trends in genetics : TIG. 2017 Mar 11. Patra BKon YYadav GSevold AWFrumkin JPVallabhajosyula RRHintze AØstman BSchossau JBhan AMarzolf BTamashiro JKKaur ABaliga NSGrayhack EJAdami CGalas DJRaval APhizicky EMRay A A genome wide dosage suppressor network reveals genomic robustness.; Nucleic acids research; Vol 45(1). 2017 Jan 09. Gamble CEBrule CEDean KMFields SGrayhack EJ Adjacent Codons Act in Concert to Modulate Translation Efficiency in Yeast.; Cell. 2016 Jun 29. Brule CEDean KMGrayhack EJ RNA-ID, a Powerful Tool for Identifying and Characterizing Regulatory Sequences.; Methods in enzymology; Vol 572. 2016. Quivey RGGrayhack EJFaustoferri RCHubbard CJBaldeck JDWolf ASMacGilvray MERosalen PLScott-Anne KSantiago BGopal SPayne JMarquis RE Functional profiling in Streptococcus mutans: construction and examination of a genomic collection of gene deletion mutants.; Molecular oral microbiology; Vol 30(6). 2015 Dec. Wolf ASGrayhack EJ Asc1, homolog of human RACK1, prevents frameshifting in yeast by ribosomes stalled at CGA codon repeats.; RNA (New York, N.Y.); Vol 21(5). 2015 May. Guy MPYoung DLPayea MJZhang XKon YDean KMGrayhack EJMathews DHFields SPhizicky EM Identification of the determinants of tRNA function and susceptibility to rapid tRNA decay by high-throughput in vivo analysis.; Genes & development; Vol 28(15). 2014 Aug 1. Letzring DPWolf ASBrule CEGrayhack EJ Translation of CGA codon repeats in yeast involves quality control components and ribosomal protein L1.; RNA (New York, N.Y.); Vol 19(9). 2013 Sep. Grant TDLuft JRWolfley JRSnell METsuruta HCorretore SQuartley EPhizicky EMGrayhack EJSnell EH The structure of yeast glutaminyl-tRNA synthetase and modeling of its interaction with tRNA.; Journal of molecular biology; Vol 425(14). 2013 Jul 24. Dean KMGrayhack EJ RNA-ID, a highly sensitive and robust method to identify cis-regulatory sequences using superfolder GFP and a fluorescence-based assay.; RNA (New York, N.Y.); Vol 18(12). 2012 Dec. Grant TDSnell EHLuft JRQuartley ECorretore SWolfley JRSnell MEHadd APerona JJPhizicky EMGrayhack EJ Structural conservation of an ancient tRNA sensor in eukaryotic glutaminyl-tRNA synthetase.; Nucleic acids research; Vol 40(8). 2012 Apr. Letzring DPDean KMGrayhack EJ Control of translation efficiency in yeast by codon-anticodon interactions.; RNA (New York, N.Y.); Vol 16(12). 2010 Dec. Quartley EAlexandrov AMikucki MBuckner FSHol WGDeTitta GTPhizicky EMGrayhack EJ Heterologous expression of L. major proteins in S. cerevisiae: a test of solubility, purity, and gene recoding.; Journal of structural and functional genomics; Vol 10(3). 2009 Sep. Chernyakov IWhipple JMKotelawala LGrayhack EJPhizicky EM Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1.; Genes & development; Vol 22(10). 2008 May 15. Kotelawala LGrayhack EJPhizicky EM Identification of yeast tRNA Um(44) 2'-O-methyltransferase (Trm44) and demonstration of a Trm44 role in sustaining levels of specific tRNA(Ser) species.; RNA (New York, N.Y.); Vol 14(1). 2008 Jan. Jackman JEGrayhack EJPhizicky EM The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins.; Methods in molecular biology (Clifton, N.J.); Vol 488. 2008. Chernyakov IBaker MAGrayhack EJPhizicky EM Chapter 11. Identification and analysis of tRNAs that are degraded in Saccharomyces cerevisiae due to lack of modifications.; Methods in enzymology; Vol 449. 2008. Malkowski MGQuartley EFriedman AEBabulski JKon YWolfley JSaid MLuft JRPhizicky EMDeTitta GTGrayhack EJ Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing.; Proceedings of the National Academy of Sciences of the United States of America; Vol 104(16). 2007 Apr 17. Wilkinson MLCrary SMJackman JEGrayhack EJPhizicky EM The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4.; RNA (New York, N.Y.); Vol 13(3). 2007 Mar. White MAClark KMGrayhack EJDumont ME Characteristics affecting expression and solubilization of yeast membrane proteins.; Journal of molecular biology; Vol 365(3). 2007 Jan 19. Jackman JEKotelawala LGrayhack EJPhizicky EM Identification and characterization of modification enzymes by biochemical analysis of the proteome.; Methods in enzymology; Vol 425. 2007. Phizicky EMGrayhack EJ Proteome-scale analysis of biochemical activity.; Critical reviews in biochemistry and molecular biology; Vol 41(5). 2006 Sep. Alexandrov AChernyakov IGu WHiley SLHughes TRGrayhack EJPhizicky EM Rapid tRNA decay can result from lack of nonessential modifications.; Molecular cell; Vol 21(1). 2006 Jan 6. Gelperin DMWhite MAWilkinson MLKon YKung LAWise KJLopez-Hoyo NJiang LPiccirillo SYu HGerstein MDumont MEPhizicky EMSnyder MGrayhack EJ Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.; Genes & development; Vol 19(23). 2005 Dec 1. Gu WHurto RLHopper AKGrayhack EJPhizicky EM Depletion of Saccharomyces cerevisiae tRNA(His) guanylyltransferase Thg1p leads to uncharged tRNAHis with additional m(5)C.; Molecular and cellular biology; Vol 25(18). 2005 Sep. Ma XYang CAlexandrov AGrayhack EJBehm-Ansmant IYu YT Pseudouridylation of yeast U2 snRNA is catalyzed by either an RNA-guided or RNA-independent mechanism.; The EMBO journal; Vol 24(13). 2005 Jul 6. Alexandrov AGrayhack EJPhizicky EM tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p.; RNA (New York, N.Y.); Vol 11(5). 2005 May. Alexandrov AVignali MLaCount DJQuartley Ede Vries CDe Rosa DBabulski JMitchell SFSchoenfeld LWFields SHol WGDumont MEPhizicky EMGrayhack EJ A facile method for high-throughput co-expression of protein pairs.; Molecular & cellular proteomics : MCP; Vol 3(9). 2004 Sep. Van Slyke CGrayhack EJ The essential transcription factor Reb1p interacts with the CLB2 UAS outside of the G2/M control region.; Nucleic acids research; Vol 31(15). 2003 Aug 1. Phizicky EMMartzen MRMcCraith SMSpinelli SLXing FShull NPVan Slyke CMontagne RKTorres FMFields SGrayhack EJ Biochemical genomics approach to map activities to genes.; Methods in enzymology; Vol 350. 2002. Grayhack EJPhizicky EM Genomic analysis of biochemical function.; Current opinion in chemical biology; Vol 5(1). 2001 Feb. Martzen MRMcCraith SMSpinelli SLTorres FMFields SGrayhack EJPhizicky EM A biochemical genomics approach for identifying genes by the activity of their products.; Science (New York, N.Y.); Vol 286(5442). 1999 Nov 5. Kuo MHNadeau ETGrayhack EJ Multiple phosphorylated forms of the Saccharomyces cerevisiae Mcm1 protein include an isoform induced in response to high salt concentrations.; Molecular and cellular biology; Vol 17(2). 1997 Feb. Kuo MHGrayhack E A library of yeast genomic MCM1 binding sites contains genes involved in cell cycle control, cell wall and membrane structure, and metabolism.; Molecular and cellular biology; Vol 14(1). 1994 Jan. Grayhack EJ The yeast alpha 1 and MCM1 proteins bind a single strand of their duplex DNA recognition site.; Molecular and cellular biology; Vol 12(8). 1992 Aug. Yang XJHart CMGrayhack EJRoberts JW Transcription antitermination by phage lambda gene Q protein requires a DNA segment spanning the RNA start site.; Genes & development; Vol 1(3). 1987 May. Grayhack EJYang XJLau LFRoberts JW Phage lambda gene Q antiterminator recognizes RNA polymerase near the promoter and accelerates it through a pause site.; Cell; Vol 42(1). 1985 Aug. Grayhack EJRoberts JW Purification of the bacteriophage lambda late gene regulator encoded by gene Q.; The Journal of biological chemistry; Vol 258(15). 1983 Aug 10. Grayhack EJRoberts JW The phage lambda Q gene product: activity of a transcription antiterminator in vitro.; Cell; Vol 30(2). 1982 Sep.