URMC / Labs / Grayhack Lab / Publications Recent Publications Brule CEGrayhack EJ (2017 Mar 11). Synonymous Codons: Choose Wisely for Expression. Trends in genetics : TIG. Patra BKon YYadav GSevold AWFrumkin JPVallabhajosyula RRHintze AØstman BSchossau JBhan AMarzolf BTamashiro JKKaur ABaliga NSGrayhack EJAdami CGalas DJRaval APhizicky EMRay A (2017 Jan 09). A genome wide dosage suppressor network reveals genomic robustness. Nucleic acids research. Gamble CEBrule CEDean KMFields SGrayhack EJ (2016 Jun 29). Adjacent Codons Act in Concert to Modulate Translation Efficiency in Yeast. Cell. Brule CEDean KMGrayhack EJ (2016). RNA-ID, a Powerful Tool for Identifying and Characterizing Regulatory Sequences. Methods in enzymology. Quivey RGGrayhack EJFaustoferri RCHubbard CJBaldeck JDWolf ASMacGilvray MERosalen PLScott-Anne KSantiago BGopal SPayne JMarquis RE (2015 Dec). Functional profiling in Streptococcus mutans: construction and examination of a genomic collection of gene deletion mutants. Molecular oral microbiology. Wolf ASGrayhack EJ (2015 May). Asc1, homolog of human RACK1, prevents frameshifting in yeast by ribosomes stalled at CGA codon repeats. RNA (New York, N.Y.). Guy MPYoung DLPayea MJZhang XKon YDean KMGrayhack EJMathews DHFields SPhizicky EM (2014 Aug 1). Identification of the determinants of tRNA function and susceptibility to rapid tRNA decay by high-throughput in vivo analysis. Genes & development. Letzring DPWolf ASBrule CEGrayhack EJ (2013 Sep). Translation of CGA codon repeats in yeast involves quality control components and ribosomal protein L1. RNA (New York, N.Y.). Grant TDLuft JRWolfley JRSnell METsuruta HCorretore SQuartley EPhizicky EMGrayhack EJSnell EH (2013 Jul 24). The structure of yeast glutaminyl-tRNA synthetase and modeling of its interaction with tRNA. Journal of molecular biology. Dean KMGrayhack EJ (2012 Dec). RNA-ID, a highly sensitive and robust method to identify cis-regulatory sequences using superfolder GFP and a fluorescence-based assay. RNA (New York, N.Y.). Grant TDSnell EHLuft JRQuartley ECorretore SWolfley JRSnell MEHadd APerona JJPhizicky EMGrayhack EJ (2012 Apr). Structural conservation of an ancient tRNA sensor in eukaryotic glutaminyl-tRNA synthetase. Nucleic acids research. Letzring DPDean KMGrayhack EJ (2010 Dec). Control of translation efficiency in yeast by codon-anticodon interactions. RNA (New York, N.Y.). Quartley EAlexandrov AMikucki MBuckner FSHol WGDeTitta GTPhizicky EMGrayhack EJ (2009 Sep). Heterologous expression of L. major proteins in S. cerevisiae: a test of solubility, purity, and gene recoding. Journal of structural and functional genomics. Chernyakov IWhipple JMKotelawala LGrayhack EJPhizicky EM (2008 May 15). Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1. Genes & development. Kotelawala LGrayhack EJPhizicky EM (2008 Jan). Identification of yeast tRNA Um(44) 2'-O-methyltransferase (Trm44) and demonstration of a Trm44 role in sustaining levels of specific tRNA(Ser) species. RNA (New York, N.Y.). Jackman JEGrayhack EJPhizicky EM (2008). The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins. Methods in molecular biology (Clifton, N.J.). Chernyakov IBaker MAGrayhack EJPhizicky EM (2008). Chapter 11. Identification and analysis of tRNAs that are degraded in Saccharomyces cerevisiae due to lack of modifications. Methods in enzymology. Malkowski MGQuartley EFriedman AEBabulski JKon YWolfley JSaid MLuft JRPhizicky EMDeTitta GTGrayhack EJ (2007 Apr 17). Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing. Proceedings of the National Academy of Sciences of the United States of America. Wilkinson MLCrary SMJackman JEGrayhack EJPhizicky EM (2007 Mar). The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4. RNA (New York, N.Y.). White MAClark KMGrayhack EJDumont ME (2007 Jan 19). Characteristics affecting expression and solubilization of yeast membrane proteins. Journal of molecular biology. Jackman JEKotelawala LGrayhack EJPhizicky EM (2007). Identification and characterization of modification enzymes by biochemical analysis of the proteome. Methods in enzymology. Phizicky EMGrayhack EJ (2006 Sep). Proteome-scale analysis of biochemical activity. Critical reviews in biochemistry and molecular biology. Alexandrov AChernyakov IGu WHiley SLHughes TRGrayhack EJPhizicky EM (2006 Jan 6). Rapid tRNA decay can result from lack of nonessential modifications. Molecular cell. Gelperin DMWhite MAWilkinson MLKon YKung LAWise KJLopez-Hoyo NJiang LPiccirillo SYu HGerstein MDumont MEPhizicky EMSnyder MGrayhack EJ (2005 Dec 1). Biochemical and genetic analysis of the yeast proteome with a movable ORF collection. Genes & development. Gu WHurto RLHopper AKGrayhack EJPhizicky EM (2005 Sep). Depletion of Saccharomyces cerevisiae tRNA(His) guanylyltransferase Thg1p leads to uncharged tRNAHis with additional m(5)C. Molecular and cellular biology. Ma XYang CAlexandrov AGrayhack EJBehm-Ansmant IYu YT (2005 Jul 6). Pseudouridylation of yeast U2 snRNA is catalyzed by either an RNA-guided or RNA-independent mechanism. The EMBO journal. Alexandrov AGrayhack EJPhizicky EM (2005 May). tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p. RNA (New York, N.Y.). Alexandrov AVignali MLaCount DJQuartley Ede Vries CDe Rosa DBabulski JMitchell SFSchoenfeld LWFields SHol WGDumont MEPhizicky EMGrayhack EJ (2004 Sep). A facile method for high-throughput co-expression of protein pairs. Molecular & cellular proteomics : MCP. Van Slyke CGrayhack EJ (2003 Aug 1). The essential transcription factor Reb1p interacts with the CLB2 UAS outside of the G2/M control region. Nucleic acids research. Phizicky EMMartzen MRMcCraith SMSpinelli SLXing FShull NPVan Slyke CMontagne RKTorres FMFields SGrayhack EJ (2002). Biochemical genomics approach to map activities to genes. Methods in enzymology. Grayhack EJPhizicky EM (2001 Feb). Genomic analysis of biochemical function. Current opinion in chemical biology. Martzen MRMcCraith SMSpinelli SLTorres FMFields SGrayhack EJPhizicky EM (1999 Nov 5). A biochemical genomics approach for identifying genes by the activity of their products. Science (New York, N.Y.). Kuo MHNadeau ETGrayhack EJ (1997 Feb). Multiple phosphorylated forms of the Saccharomyces cerevisiae Mcm1 protein include an isoform induced in response to high salt concentrations. Molecular and cellular biology. Kuo MHGrayhack E (1994 Jan). A library of yeast genomic MCM1 binding sites contains genes involved in cell cycle control, cell wall and membrane structure, and metabolism. Molecular and cellular biology. Grayhack EJ (1992 Aug). The yeast alpha 1 and MCM1 proteins bind a single strand of their duplex DNA recognition site. Molecular and cellular biology. Yang XJHart CMGrayhack EJRoberts JW (1987 May). Transcription antitermination by phage lambda gene Q protein requires a DNA segment spanning the RNA start site. Genes & development. Grayhack EJYang XJLau LFRoberts JW (1985 Aug). Phage lambda gene Q antiterminator recognizes RNA polymerase near the promoter and accelerates it through a pause site. Cell. Grayhack EJRoberts JW (1983 Aug 10). Purification of the bacteriophage lambda late gene regulator encoded by gene Q. The Journal of biological chemistry. Grayhack EJRoberts JW (1982 Sep). The phage lambda Q gene product: activity of a transcription antiterminator in vitro. Cell.