URMC / Labs / Grayhack Lab / Publications Recent Publications Houston LPlatten EMConnelly SMWang JGrayhack EJ Frameshifting at collided ribosomes is modulated by elongation factor eEF3 and by Integrated Stress Response regulators Gcn1 and Gcn20.; RNA (New York, N.Y.). 2021 Dec 16. Ghoneim DHZhang XBrule CEMathews DHGrayhack EJ Conservation of location of several specific inhibitory codon pairs in the Saccharomyces sensu stricto yeasts reveals translational selection.; Nucleic acids research. 2018 Dec 21. Wang JZhou JYang QGrayhack EJ Multi-protein Bridging Factor 1(Mbf1), Rps3 and Asc1 prevent stalled ribosomes from frameshifting.; eLife; Vol 7. 2018 Nov 22. Coller JGrayhack EJ The analysis of translational mechanisms that modulate gene expression.; Methods (San Diego, Calif.); Vol 137. 2018 Mar 15. Brule CEGrayhack EJ Synonymous Codons: Choose Wisely for Expression.; Trends in genetics : TIG. 2017 Mar 11. Patra BKon YYadav GSevold AWFrumkin JPVallabhajosyula RRHintze AØstman BSchossau JBhan AMarzolf BTamashiro JKKaur ABaliga NSGrayhack EJAdami CGalas DJRaval APhizicky EMRay A A genome wide dosage suppressor network reveals genomic robustness.; Nucleic acids research; Vol 45(1). 2017 Jan 09. Gamble CEBrule CEDean KMFields SGrayhack EJ Adjacent Codons Act in Concert to Modulate Translation Efficiency in Yeast.; Cell. 2016 Jun 29. Brule CEDean KMGrayhack EJ RNA-ID, a Powerful Tool for Identifying and Characterizing Regulatory Sequences.; Methods in enzymology; Vol 572. 2016. Quivey RGGrayhack EJFaustoferri RCHubbard CJBaldeck JDWolf ASMacGilvray MERosalen PLScott-Anne KSantiago BGopal SPayne JMarquis RE Functional profiling in Streptococcus mutans: construction and examination of a genomic collection of gene deletion mutants.; Molecular oral microbiology; Vol 30(6). 2015 Dec. Wolf ASGrayhack EJ Asc1, homolog of human RACK1, prevents frameshifting in yeast by ribosomes stalled at CGA codon repeats.; RNA (New York, N.Y.); Vol 21(5). 2015 May. Guy MPYoung DLPayea MJZhang XKon YDean KMGrayhack EJMathews DHFields SPhizicky EM Identification of the determinants of tRNA function and susceptibility to rapid tRNA decay by high-throughput in vivo analysis.; Genes & development; Vol 28(15). 2014 Aug 1. Letzring DPWolf ASBrule CEGrayhack EJ Translation of CGA codon repeats in yeast involves quality control components and ribosomal protein L1.; RNA (New York, N.Y.); Vol 19(9). 2013 Sep. Grant TDLuft JRWolfley JRSnell METsuruta HCorretore SQuartley EPhizicky EMGrayhack EJSnell EH The structure of yeast glutaminyl-tRNA synthetase and modeling of its interaction with tRNA.; Journal of molecular biology; Vol 425(14). 2013 Jul 24. Dean KMGrayhack EJ RNA-ID, a highly sensitive and robust method to identify cis-regulatory sequences using superfolder GFP and a fluorescence-based assay.; RNA (New York, N.Y.); Vol 18(12). 2012 Dec. Grant TDSnell EHLuft JRQuartley ECorretore SWolfley JRSnell MEHadd APerona JJPhizicky EMGrayhack EJ Structural conservation of an ancient tRNA sensor in eukaryotic glutaminyl-tRNA synthetase.; Nucleic acids research; Vol 40(8). 2012 Apr. Letzring DPDean KMGrayhack EJ Control of translation efficiency in yeast by codon-anticodon interactions.; RNA (New York, N.Y.); Vol 16(12). 2010 Dec. Quartley EAlexandrov AMikucki MBuckner FSHol WGDeTitta GTPhizicky EMGrayhack EJ Heterologous expression of L. major proteins in S. cerevisiae: a test of solubility, purity, and gene recoding.; Journal of structural and functional genomics; Vol 10(3). 2009 Sep. Chernyakov IWhipple JMKotelawala LGrayhack EJPhizicky EM Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1.; Genes & development; Vol 22(10). 2008 May 15. Kotelawala LGrayhack EJPhizicky EM Identification of yeast tRNA Um(44) 2'-O-methyltransferase (Trm44) and demonstration of a Trm44 role in sustaining levels of specific tRNA(Ser) species.; RNA (New York, N.Y.); Vol 14(1). 2008 Jan. Jackman JEGrayhack EJPhizicky EM The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins.; Methods in molecular biology (Clifton, N.J.); Vol 488. 2008. Chernyakov IBaker MAGrayhack EJPhizicky EM Chapter 11. Identification and analysis of tRNAs that are degraded in Saccharomyces cerevisiae due to lack of modifications.; Methods in enzymology; Vol 449. 2008. Malkowski MGQuartley EFriedman AEBabulski JKon YWolfley JSaid MLuft JRPhizicky EMDeTitta GTGrayhack EJ Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing.; Proceedings of the National Academy of Sciences of the United States of America; Vol 104(16). 2007 Apr 17. Wilkinson MLCrary SMJackman JEGrayhack EJPhizicky EM The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4.; RNA (New York, N.Y.); Vol 13(3). 2007 Mar. White MAClark KMGrayhack EJDumont ME Characteristics affecting expression and solubilization of yeast membrane proteins.; Journal of molecular biology; Vol 365(3). 2007 Jan 19. Jackman JEKotelawala LGrayhack EJPhizicky EM Identification and characterization of modification enzymes by biochemical analysis of the proteome.; Methods in enzymology; Vol 425. 2007. Phizicky EMGrayhack EJ Proteome-scale analysis of biochemical activity.; Critical reviews in biochemistry and molecular biology; Vol 41(5). 2006 Sep. Alexandrov AChernyakov IGu WHiley SLHughes TRGrayhack EJPhizicky EM Rapid tRNA decay can result from lack of nonessential modifications.; Molecular cell; Vol 21(1). 2006 Jan 6. Gelperin DMWhite MAWilkinson MLKon YKung LAWise KJLopez-Hoyo NJiang LPiccirillo SYu HGerstein MDumont MEPhizicky EMSnyder MGrayhack EJ Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.; Genes & development; Vol 19(23). 2005 Dec 1. Gu WHurto RLHopper AKGrayhack EJPhizicky EM Depletion of Saccharomyces cerevisiae tRNA(His) guanylyltransferase Thg1p leads to uncharged tRNAHis with additional m(5)C.; Molecular and cellular biology; Vol 25(18). 2005 Sep. Ma XYang CAlexandrov AGrayhack EJBehm-Ansmant IYu YT Pseudouridylation of yeast U2 snRNA is catalyzed by either an RNA-guided or RNA-independent mechanism.; The EMBO journal; Vol 24(13). 2005 Jul 6. Alexandrov AGrayhack EJPhizicky EM tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p.; RNA (New York, N.Y.); Vol 11(5). 2005 May. Alexandrov AVignali MLaCount DJQuartley Ede Vries CDe Rosa DBabulski JMitchell SFSchoenfeld LWFields SHol WGDumont MEPhizicky EMGrayhack EJ A facile method for high-throughput co-expression of protein pairs.; Molecular & cellular proteomics : MCP; Vol 3(9). 2004 Sep. Van Slyke CGrayhack EJ The essential transcription factor Reb1p interacts with the CLB2 UAS outside of the G2/M control region.; Nucleic acids research; Vol 31(15). 2003 Aug 1. Phizicky EMMartzen MRMcCraith SMSpinelli SLXing FShull NPVan Slyke CMontagne RKTorres FMFields SGrayhack EJ Biochemical genomics approach to map activities to genes.; Methods in enzymology; Vol 350. 2002. Grayhack EJPhizicky EM Genomic analysis of biochemical function.; Current opinion in chemical biology; Vol 5(1). 2001 Feb. Martzen MRMcCraith SMSpinelli SLTorres FMFields SGrayhack EJPhizicky EM A biochemical genomics approach for identifying genes by the activity of their products.; Science (New York, N.Y.); Vol 286(5442). 1999 Nov 5. Kuo MHNadeau ETGrayhack EJ Multiple phosphorylated forms of the Saccharomyces cerevisiae Mcm1 protein include an isoform induced in response to high salt concentrations.; Molecular and cellular biology; Vol 17(2). 1997 Feb. Kuo MHGrayhack E A library of yeast genomic MCM1 binding sites contains genes involved in cell cycle control, cell wall and membrane structure, and metabolism.; Molecular and cellular biology; Vol 14(1). 1994 Jan. Grayhack EJ The yeast alpha 1 and MCM1 proteins bind a single strand of their duplex DNA recognition site.; Molecular and cellular biology; Vol 12(8). 1992 Aug. Yang XJHart CMGrayhack EJRoberts JW Transcription antitermination by phage lambda gene Q protein requires a DNA segment spanning the RNA start site.; Genes & development; Vol 1(3). 1987 May. Grayhack EJYang XJLau LFRoberts JW Phage lambda gene Q antiterminator recognizes RNA polymerase near the promoter and accelerates it through a pause site.; Cell; Vol 42(1). 1985 Aug. Grayhack EJRoberts JW Purification of the bacteriophage lambda late gene regulator encoded by gene Q.; The Journal of biological chemistry; Vol 258(15). 1983 Aug 10. Grayhack EJRoberts JW The phage lambda Q gene product: activity of a transcription antiterminator in vitro.; Cell; Vol 30(2). 1982 Sep.