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URochester Medicine / Microbiology & Immunology / Research / Xenopus Laevis / Primers Available

Primers Available

Conventional PCR primer list for X. laevis house keeping and other genes

Primers name Primer direction and/or sequence aSize (bp)TM (oC)
Adult beta-globinF: 5’ - GACAGCACATGATCGTCAGC- 3’
F: 5’ - CTTCATGGAAGTGGCGAGCC- 3’		58351
EF-1αF: 5’ - CCTGAATCACCCAGGCCAGATTGGTG - 3’
R: 5’ - GAGGGTAGTGTGAGAAGCTCTCCACG - 3’		25056
GAPDHF: 5’ - ACCCCTTCATCGACTTGGAC - 3’
R: 5’ - GGAGCCAGACAGTTTGTAGTG - 3’		30055
Gp96F: 5’ - GCGTTGGTTTCTATTCTGCCTTC- 3’
F: 5’ - TGTCTCAGTCTTGCTGCTCCACAC- 3’		56400
Hsp72F: 5’ - AGACTGATCGGGAGAAAGTTC - 3’
R: 5’ - CTTTTCCACAGGTTCCAAGGTACCC - 3’		58747
Hsc73F: 5’ - TGGAGTAGAAAGGAATGTACTGATC - 3’
R: 5’ - AACTTCATTGCATTTCTCCAG - 3’		581174
LMPXF: 5’ - TATCAGTGTCTTCCGCCTCC - 3’
R: 5’ - CAGCCGTCCTCCTTCATGTG - 3’		56-64300

Conventional PCR primer list for Xenopus laevis immune receptor genes

PrimerSequence (5’-3’ forward/reverse)Tm (°C)Size (bp)
b2-microglobulin consensusF: 5’ - TGACGGTGAATCCTGGAGAC- 3’
R: 5’ - CGATAGCCGTGACAATGAGC- 3’		57800
CD4F: 5’ - TCCATCTCTGACATCCCCTC- 3’
R: 5’ - TCACCAGACACACGTCCATT- 3’		60895
CD8 aF: 5’ - AAGCCACCTACGACTACCACCAA- 3’
R: 5’ - CCGTTCTTCTCAGTCTCAGGCAC- 3’		59247
CD8 βF: 5’ - TCATCATCTCTTTCTGGGGC- 3’
R: 5’ - AATTCAGTGGGTGCTTCCTG- 3’		60604
CTXF: 5’ - GCAgcagcggtaatcggag- 3’
R: 5’ - ctcagcatggtcatggaattg- 3’		58220
Ig YF: 5’ - ATGGAGGAGCCAACTCACCATC- 3’
R: 5’ - AAGGTGGCAGCAAGGTGATTG- 3’		58720
Ig MF: 5’ - TCTTCTCTTGTAAGGAACCCG- 3’
R: 5’ - GCAATGCCAAACACCTGG- 3’		58350
MHC class Ia
f strain		F: 5’ - GAGTTCTTTGCCCTGGATACA- 3’
R: 5’ - CACAATAAGTTTCTCCTTCAGG- 3’		61400
MHC class Ia
j strain		F: 5’ - GAGTTCATAGCGCTGGATACA- 3’
R: 5’ - CCTCACTGGGTGTAATCTCAG- 3’		61400
MHC II a chainF: 5’ - TCAAAGTCAGTGGTTTGGACG- 3’
R: 5’ - GTGTAAATATCATGTTCATTTG- 3’		61390
MHC II b chainF: 5’ - ACGGCACCGACAATGTCAGG- 3’
TTAATGGGTGTCTCCAGCATC- 3’		61450
TCR aF: 5’ - TTCAGGGACCTCTGGTTGCTTC- 3’
R: 5’ - ACACATCAGCCTCAATCCATCC- 3’		60 
TCR V b 1F: 5’ - CACCCAGGAGCCAAGATC- 3’56700
TCR V b 3F: 5’ - TGACGGTGAATCCTGGAGAC- 3’58700
TCR V b 6F: 5’ - GTCAGCTGACATTCCTTGTAATC- 3’58700
TCR V b 8F: 5’ - GACCAAAAGTTCTTAGCG- 3’50700
TCR b constantR: 5’ - CGATAGCCGTGACAATGAGC- 3’50-58700
TCR gF: 5’ - AGAAGTTCTCAAGCAGGCAG- 3’
R: 5’ - AAGTCAGCTGAAGAGGATCC- 3’		60328
XNC 2F: 5’ - GGTATCAAACCGTATCTATGGTG- 3’
R: 5’ - TCTCTATATACTCCTTACTATCAG- 3’		59450
XNC 6F: 5’ - CTTTGCATGATCGTAATG- 3’
R: 5’ - CTGCATATCTAGATGGCTTG- 3’		52386
XNC 7F: 5’ - CCGATGTGAGCCTTTGTTTCAG- 3’
R: 5’ - GTGCACTCATTTTCCATGAATAGT- 3’		59380
XNC 11F: 5’ - CGATGACTTCTTCACCGACC- 3’
R: 5’ - CCTTGCTCCACTCTGGTATG- 3’		58350

Conventional PCR primer list for X. laevis cytokine and other genes

Primers name Primer direction and/or sequence aSize (bp)TM (oC)
AIDF: 5’ -  TCACGACCCCCATAGGAACTAC - 3’
R: 5’ -  TTAGGAGACTTTGCCTCAAGG - 3’		45058
Arginase IF: 5’ - CTTACAATGAGTAGTAGCCAAGG - 3’
R: 5’ - GCAGAGAAAATGAGGATTCG - 3’		37856
IL-1βF: 5’ - AACAGAAGATGGCCAAGACTC - 3’
R: 5’ - ATGCAACCGATTCAAAGC - 3’		15060
Type II IFN (gamma)F: 5’ - CTGAGGAAATACTTTAACTCCATTGACC - 3’
R: 5’ - TTGTAACATCTCCCACCTGTATTGTC - 3’		30060
MX1F: 5’ - AGGAGTGGTCAACAGGAGCC- 3’
R: 5’ - TGTTCCGCCGCTGTTCCTCT- 3’		82968
MX2F: 5’ - GGAACGCCGCACTTGCAGAA- 3’
R: 5’ - CGATTAATCCTGGCACCTCC- 3’		27468
TNF-αF: 5’ - GCTCAAGGATAACTCCATCG - 3’
R: 5’ - AACCAAGTGGCACCTGAATG - 3’		20359
Rag1F: 5’ - GCGCCAAGAATCTGTGTCACT- 3’
R: 5’ - GTTCTGTTTCATGGTTGTCTACCA- 3’		35058
Mycobacterium marinumF: 5’ - AGAGTTTGATCCTGGCTCAG - 3’
R: 5’ - CACGCTCACAGTTAAGCCGT - 3’		37856
DM-W (sex determination)F: 5’ - CCACACCCAGCTCATGTAAAG- 3’
R: 5’ - GGGCAGAGTCACATATACTG- 3’		26052

Conventional PCR primer list for pathogens of X. laevis

Primers name Primer direction and/or sequence a
Ranavirus FV3		Size (bp)TM (oC)
vDNA polymerase II (60R)F: 5’ - ACGAGCCCGACGAAGACTACATAG - 3’
R: 5’ - TGGTGGTCCTCAGCATCCTTTG - 3’		37856
MCPF: 5’ - GACTTGGCC ACTTATGAC - 3’
R: 5’ - GTCTCTGGAGAAGAAGAAGAA - 3’		52552
vCARD (64R)

 

F: 5’ - AGACCCTCCAGAACCTGATTGAC - 3’
R: 5’ - TCTCCCTTCTTGGTGACGCTGT - 3’		15059
vβHSD (52L)F: 5’ - CACTGTCCACGATCACGTCTACC - 3’
R: 5’ - AACTTTACGCCCGTCACCCTCA - 3’		55060
vICP-18 (18K)F: 5’ - ATGATCCAAGCCTACCTGTGC- 3’
R: 5’ - AAATGTCCTAATCTATACACC- 3’		45052
veIF-2α (vIF-2α)F: 5’ - CTGTAAATGTTTAGACTTGGAGTG- 3’
R: 5’ - ACACAAAGGGGCACAGTCCTCC- 3’		  
53RF: 5’ - ATCAGTCACAGGTCTTTAGCATAG- 3’
R: 5’ - TCTGCTTGATCTCGGGACACAATG- 3’		44156
EGFPF: 5’ - ACGGCCACAAGTTCAGCGTG - 3’
R: 5’ - GTCCATGCCGAGAGTGATCC - 3’		35056
M. marinumF: 5’ - AGAGTTTGATCCTGGCTCAG - 3’
R: 5’ - CACGCTCACAGTTAAGCCGT  - 3’		37856
ChytridF: 5’ - AGCCAAGAGATCCGTTGTCAAA- 3’
R: 5’ - CCTTGATATAATACAGTGTGCCATATC- 3’		25060
CTXF: 5’ - GCAGCAGCGGTAATCGGAG- 3’
R: 5’ - CTCAGCATGGTCATGGAATTG- 3’		22058

Conventional Q-PCR primer list for X. laevis genes

Primers Primer direction and/or sequence
GAPDHF: 5’ - GACATCAAGGCCGCCATTAAGACT - 3’
R: 5’ - AGATGGAGGAGTGAGTGTCACCAT - 3’
EF-1αF: 5’ - GCCTTCCCAGGGTTCAACAT - 3’
R: 5’ - TCGGTGGGTCACTCTTTGAAT  - 3’
GCSF-RF: 5’ - ACGTGCCAGCTA AACCTCACAGAT - 3’
R: 5’ - TGA CACAGCCTGGGCGAGAAATAA  - 3’
IL-1βF: 5’ - CATTCCCATGGAGGGCTACA - 3’
R: 5’ - TGACTGCCACTGAGCAGCAT - 3’
IL-10F: 5’ - TGCTGGATCTTAAGCACACCCTGA - 3’
R: 5’ - TGTACAGGCCTTGTTCACGCATCT - 3’
Type I IFNF: 5’ - GCTGCTCCTGCTCAGTCTCA- 3’
R: 5’ - GAAAGCCTTCAGGATCTGTGTGT- 3’
Type II IFN (gamma)F: 5’ - CTGAGGAAATACTTTAACTCCATTGACC - 3’
R: 5’ - TTGTAACATCTCCCACCTGTATTGTC- 3’
Type III IFN (lambda)F: 5’ - TCCCTCCCAACAGCTCATG- 3’
R: 5’ - CCGACACACTGAGCGGAAA - 3’
IFN lambda receptorF: 5’ - GGAGCCTGATCCCAATGAATTA- 3’
(IFNLR1)R: 5’ - TCTCAAAGCGCACACTAAGG- 3’
MCSF-RF: 5’ - TGTATTCTTTGG ACT TGC CGT ATCTGG - 3’
R: 5’ - TTGTTTAGCTTCAAATTCTGGGTAATA - 3
MX1F: 5’ - AGCAGTGGTCAACAGGAGCC - 3’
R: 5’ - TGTTCCGCCGCTGTTCCTCT - 3
MX2F: 5’ - GGAACGCCGCACTTGCAGAA - 3’
R: 5’ - CGATTAATCCTGGCACCTCC - 3
TNF-αF: 5’ - TGTCAGGCAGGAAAGAAGCA - 3’
R: 5’ - CAGCAGAGCAAAGAGGATGGT- 3’
HSP70F: 5’ - CTCATCAAGCGCAACACAAC - 3’
R: 5’ - CTCCCTCAAACACCTGGATAAG- 3’
Glucocorticoids (GCs) (CYP11A1)F: 5’ - GGAGGAGTAGACACGACATCTA - 3’
R: 5’ - GGCAGTAAGATCCTTTCCAGAG- 3’
Thyroid hormone receptor α (THRα)F: 5’ - GTTCTGGATGGAATTACG - 3’
R: 5’ - CATACATTGGCTGTTCTT- 3’
IL-34F: 5’ - AGCTCTTCTACGTGTATTCCTTGG - 3’
R: 5’ - TGATAAGCGATTGACCTACCTGGG - 3’
iNOSF: 5’ - AACCGTAAGCCAAAGAAGGA - 3’
R: 5’ - TGGTTCTGGCAGCCACAGT - 3’
FoxP3 (Forkhead box P3)F: 5’ - AGTGGTGGGCAGTTGTATG - 3’
R: 5’ - AGGTTCCGCATGTAGAAGATG - 3’
IL-1β (interlukin 1 beta)F: 5’ - CATTCCCATGGAGGGCTACA - 3’
R: 5’ - TGACTGCCACTGAGCAGCAT - 3’
IL-4/13F: 5’ - GCAATTAACGCTTCCCTTTCC - 3’
R: 5’ - AGAAGATCGTCCCTGACTGA - 3’
IL-6 (Interlukin 6)F: 5’ - CTGTGGAGTCCTTACAGTACAAG - 3’
R: 5’ - TGGTTCCTTTGTCATCACTCTC - 3’
IL-10 (Interlukin 10)F: 5’ - TGCTGGATCTTAAGCACACCCTGA - 3’
R: 5’ - TGTACAGGCCTTGTTCACGCATCT - 3’
IL-12β (Interlukin 12 beta)F: 5’ - GAAACCTGCTGTCCTTCTCTT - 3’
R: 5’ - TCATGGCAGTTCACCTCATATT - 3’
IL-15 SET 1 (Interlukin 15)F: 5’ - TCTCAACATCTCACCCACATTC - 3’
R: 5’ - ACTCTTCAAATGCCGCTATAAATTC - 3’
IL-15 SET2F: 5’ - GAAAGCTAAAGATACAGGAGAACAAAG - 3’
R: 5’ - TGTGTGAATGTGGGTGAGATG - 3’
IL-18 (Interlukin 18)F: 5’ - CAGCAAGTGTTCTGACAACAAG - 3’
R: 5’ - TTCACTCGCTTGAGACTGTATG - 3’
TGFβ SET 1 (Transforming growth factor beta)F: 5’ - CCCACAGGCCAAAGATATAGAC - 3’
R: 5’ - CATCAGGTAGGGTTTCGTGTT - 3’
TGFβ SET 2F: 5’ - CTACCTGTAAGGCTGTGGATATG - 3’
R: 5’ - CTGTCATCTTCTCGCTGTCTAC - 3’
CCR-2 SET 1F: 5’ - ATTGGGCAGAACTGTGGTAG - 3’
R: 5’ - GGGCGAGTAATCTGAGTCATAA - 3’
CCR-2 SET 2F: 5’ - GTCCCATGATGTCAGAGAGTAAC - 3’
R: 5’ - GTGTGGGCAGAGGCAATA - 3’
Pathogens 
FV3 vDNA Polymerase IIF: 5’ - AACCCTCGCCTGGCATCTA - 3’
(60R)R: 5’ - TGGTGGTCCTCAGCATCCT - 3’
FV3-PKRF: 5’ - GCTCACCGGCGGGATTA - 3’
R: 5’ - TTCAACTTTATTCATGCGTGCTATC - 3’
M. marinumF: 5’ - AGAGTTTGATCCTGGCTCAG- 3’
R: 5’ - CACTCGAGTATCTCCGAAGA - 3’
FV3-MCP-90R  (Major Capsid Protein)F: 5’ - GTCCTTTAACACGGCATACCT- 3’
R: 5’ - ATCGCTGGTGTTGCCTATC - 3’
FV3-94L (Clostridium tetani)F: 5’ - CGGGACCTCTATGAGCTTTATG- 3’
R: 5’ - CTACGAGTACGTCTCTGTGTTC - 3’
Immediate-early protein ICP-18K
(82R) – immediate early		F: 5’ - TGTGTCTGGAGAACCCTACA - 3’
R: 5’ - TGGTGATCTTGACTTGAAACTC - 3’
93L – late unknown functionF: 5’ - GCCAAGACCGAGGACAC - 3’
R: 5’ - GGACAAACCAACAGTAGAAACG - 3’
DNA Repair protein RAD2
(95R) –delayed early		F: 5’ - CATGTCAACGTCAAAGTCCAAG- 3’
R: 5’ - TGTGCACCCTCTGGTTAAAG - 3’
vCARD Caspase Recruitment domain (64R) – immediate earlyF: 5’ - CGCGACCGTGATGGATAAA - 3’
R: 5’ - GCCAGATGCGGATCATCTT - 3’
Perkinsea (parasite)F: 5’ - GAACGACCGTCCTACCTTGG - 3’
R: 5’ - AGGCCTGCTTGAAACACTCT - 3’

Non-polymorphic MHC class Ib (XNC) and invariant TCR alpha rearrangements

XNCs and iVT cells 
Vα6-Jα1.43F: 5’ - GGAATTGCTGGTTAATAATTTGGGA - 3’
R: 5’ - AAATGTGAGTTTGTGGAACCCCC - 3’
Vα22-Jα1.32F: 5’ - ATGTGGTGGACGGACATACAG   - 3’
R: 5’ - TGGAACCAGCTCCAGCATTAG   - 3’
Vα23-Jα1.3F: 5’ - CGCGATAAACTCAAAGGAAGAATA   - 3’
R: 5’ - CTTTCCATAGCCTATTCCAGTA  - 3’
Vα40-Jα1.22F: 5’ - TCCAGCCTACCATGGAAGAAG - 3’
R: 5’ - CATATTTCCATAGCCACCAGTACT - 3’
Vα41-Jα1.40F: 5’ -  CCCAGCCTCCCAAGAAGGAGGTC - 3’
R: 5’ -  GGTAAGTTTATTCCAGCCTCCTGT - 3’
Vα45-Jα1.14F: 5’ - TCCGTTAAAGAGAAGGATTCCCAG - 3’
R: 5’ - CTCCCAGCCACTACCAGAATAAG - 3’
Vα39 SET 1F: 5’ - ATATCTATGTGCTCTGCGGG - 3’
R: 5’ - GAGGCGATACATTGAAGGTT - 3’
Vα39 SET 2F: 5’ - CAGAATATCACACCGGAGGA - 3’
R: 5’ - TGGCTGTCACTGTCACTATT - 3’
XNC1F: 5’ - CATTTGAGGTGTGGCAAAGG - 3’
R: 5’ - GCAGCTCACATGCAGATTTC - 3’
XNC1F: 5’ - GGGCTTTCCCAGTATGCCATT - 3’
R: 5’ - CAGCCTGTGCCTTTGCCACACC - 3’