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URMC / Microbiology & Immunology / Xenopus Laevis / Resources Available

Resources Available

Reagents available upon request

Xenopus Lymphocyte Surface Markers Detectable with Available mAbs

Xenopus markers (mAbs) Expression Pattern
CD8
(AM22, F17)
Larval and adult thymocytes (70-80%) and T-cells (about 20% of splenocytes). All lymphoid tumor lines
CTX
(X71, 1S9.2)
Larval and adult thymocytes (60-70%)no consistent expression in periphery. All lymphoid tumor lines and gut epithelial tissue.
XT1
(XT1)
Most, but not all, larval and adult T-cells; earliest marker of thymocytes. All lymphoid tumor lines
MHC class I
(TB17)
Ubiquitous in adult; all lymphopoietic lineages. Not expressed until metamorphosis
MHC class II
(AM20, 14A2)
Thymocytes, B and T-cells (99% of spleen lymphocytes), only B-cells in larvae
CD5
(2B1)
Thymocytes (>95%), T-cells and some PMA-activated IgM+ B cells. All lymphoid tumor lines
NK-like
(1F8)
Non-B and non-T, peripheral lymphoid cells.
RC47 Leukocyte lineage from very early stage. Thymic cortex and medulla (>90% of total thymocytes)
IgM
(10A9)
Larval and adult B cells.
IgY
(11D5)
Some larval and adult B cells.
IgX
(410D9)
Some larval and adult B cells, especially in the gut.

cDNA Encoding Immunologically-relevant X. laevis Genes

Immune System Characteristics Larva (Ancestral-like system)
Adult (Mammalian-like system)
CTX Thymus, all lymphoid tumor lines and gut epithelial tissue.
TCR α, β, γ thymus, circulating T-cells
BCR μ, γ, ξ, L B cells
MHC class I ubiquitous in adult
Non-classical class Ib more restricted tissue distribution for some of them
MHC class II
MHC class II thymus, splenic lymphocytes
Rag 1 & 2 mainly thymus, at lower level in bone marrow, kidney, spleen and liver
TdT mainly thymus of old larvae and adult, less in spleen
Ikaros mainly thymus
GATA III thymus and T-cells
LMP7 Thymus, spleen
Hsps (gp96, hsp70) Ubiquitousely expressed

Animals currently available (embryos, tadpoles or adults)

MHC homozygous strain

MHC haplotypes

Inbred J

j/j

Partially inbred F

f/f

F/J cross

f/j

Isogenetic laevis/gilli (LG) clones

MHC haplotypes

LG-15

a/c

LG-6

a/c

Transgenic lines

Genetic background

xlurp:GFP

Outbred and J (F3)

flk:GFP

Outbred

Mpeg:Cherry/xlurp-GFP

Outbred

Silenced b2m (shRNA)

Outbred and LG-15

Silenced XNC10 (shRNA)

Outbred and LG-15

Other Xenopus species

 

X. borealis

 

Cloned Thymic Tumor Cell Lines

Tumor Cell Line BB7 ff-2 15/0
Genetic background of initial tumor-bearing host Partially inbred MHC
homozygous ff strain
LG-15 (MHC a/c) isogenetic clone
T-cell surface markers (CD8, CD5, pan T-cell) +++ +++ +++
Ig mRNA - + +
Ig protein - - -
MHC class I mRNA and protein - + -
MHC class II mRNA and protein - - -
Tumorigenicity in syngeneic
engaLarvae - + ++
Adult      

Genomic and cDNA Libraries Genomic and cDNA Libraries

X. laevis cDNA libraries/Old libraries in λZAPII vector Description ST: Outbred adult spleen + thymus (constructed by L. Du Pasquier, Basel Switzerland)) LG7Ad: Adult from the clone LG7 immunized with DNP-KLH + CFA constructed L. Du Pasquier) LG7 Tadp: LG7 pre-metamorphic larvae immunized DNP-KLH + alum (constructed by M. Wilson, Jakson MS) SMART cDNA libraries
(λZAP-express, constructed by S. Guselnikov and A. Taranin,
Novosibirsk, Russia) Description T: Outbred larval spleen + thymus + liver. 7x105 independent clones, titer 6x109 S: Outbred adult spleen. 2x106 independent clones, titer 1x109 NK: NK cells, 1F8+ purified from outbred adult splenocytes. 3x106 independent clones, titer 1x1010 T: CD8 NK/T cells (AM22+/1F8+) purified from outbred splenocytes. 5x105 independent clones, titer 4x109 Arrayed EST cDNA libraries
(constructed by Express Genomics, Inc.) Outbred adult thymus
Outbred adult splenocytes activated for 4 days with PHA X. tropicalis
cDNA libraries (λZAP-express) Trop: Adults spleen + thymus + intestine. Titer 5x1010