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Resources Available

Xenopus Lymphocyte Surface Markers Detectable with Available mAbs

Xenopus markers (mAbs)	Expression Pattern
CD8 (AM22, F17)	Larval and adult thymocytes (70-80%) and T-cells (about 20% of splenocytes). All lymphoid tumor lines
CTX (X71, 1S9.2)	Larval and adult thymocytes (60-70%)no consistent expression in periphery. All lymphoid tumor lines and gut epithelial tissue.
XT1 (XT1)	Most, but not all, larval and adult T-cells; earliest marker of thymocytes. All lymphoid tumor lines
MHC class I (TB17)	Ubiquitous in adult; all lymphopoietic lineages. Not expressed until metamorphosis
MHC class II (AM20, 14A2)	Thymocytes, B and T-cells (99% of spleen lymphocytes), only B-cells in larvae
CD5 (2B1)	Thymocytes (>95%), T-cells and some PMA-activated IgM+ B cells. All lymphoid tumor lines
NK-like (1F8)	Non-B and non-T, peripheral lymphoid cells.
RC47	Leukocyte lineage from very early stage. Thymic cortex and medulla (>90% of total thymocytes)
IgM (10A9)	Larval and adult B cells.
IgY (11D5)	Some larval and adult B cells.
IgX (410D9)	Some larval and adult B cells, especially in the gut.

cDNA Encoding Immunologically-relevant X. laevis Genes

Immune System Characteristics	Larva (Ancestral-like system) Adult (Mammalian-like system)
CTX	Thymus, all lymphoid tumor lines and gut epithelial tissue.
TCR α, β, γ	thymus, circulating T-cells
BCR μ, γ, ξ, L	B cells
MHC class I	ubiquitous in adult
Non-classical class Ib	more restricted tissue distribution for some of them MHC class II
MHC class II	thymus, splenic lymphocytes
Rag 1 & 2	mainly thymus, at lower level in bone marrow, kidney, spleen and liver
TdT	mainly thymus of old larvae and adult, less in spleen
Ikaros	mainly thymus
GATA III	thymus and T-cells
LMP7	Thymus, spleen
Hsps (gp96, hsp70)	Ubiquitousely expressed

Animals currently available (embryos, tadpoles or adults)

MHC homozygous strain	MHC haplotypes
Inbred J	j/j
Partially inbred F	f/f
F/J cross	f/j
Isogenetic laevis/gilli (LG) clones	MHC haplotypes
LG-15	a/c
LG-6	a/c
Transgenic lines	Genetic background
xlurp:GFP	Outbred and J (F3)
flk:GFP	Outbred
Mpeg:Cherry/xlurp-GFP	Outbred
Silenced b2m (shRNA)	Outbred and LG-15
Silenced XNC10 (shRNA)	Outbred and LG-15
Other Xenopus species
X. borealis

Cloned Thymic Tumor Cell Lines

Tumor Cell Line	BB7	ff-2	15/0
Genetic background of initial tumor-bearing host	Partially inbred MHC homozygous ff strain	LG-15 (MHC a/c) isogenetic clone
T-cell surface markers (CD8, CD5, pan T-cell)	+++	+++	+++
Ig mRNA	-	+	+
Ig protein	-	-	-
MHC class I mRNA and protein	-	+	-
MHC class II mRNA and protein	-	-	-
Tumorigenicity in syngeneic
engaLarvae	-	+	++
Adult

Genomic and cDNA Libraries Genomic and cDNA Libraries

X. laevis cDNA libraries/Old libraries in λZAPII vector Description ST: Outbred adult spleen + thymus (constructed by L. Du Pasquier, Basel Switzerland)) LG7Ad: Adult from the clone LG7 immunized with DNP-KLH + CFA constructed L. Du Pasquier) LG7 Tadp: LG7 pre-metamorphic larvae immunized DNP-KLH + alum (constructed by M. Wilson, Jakson MS) SMART cDNA libraries
(λZAP-express, constructed by S. Guselnikov and A. Taranin,
Novosibirsk, Russia) Description T: Outbred larval spleen + thymus + liver. 7x105 independent clones, titer 6x109 S: Outbred adult spleen. 2x106 independent clones, titer 1x109 NK: NK cells, 1F8+ purified from outbred adult splenocytes. 3x106 independent clones, titer 1x1010 T: CD8 NK/T cells (AM22+/1F8+) purified from outbred splenocytes. 5x105 independent clones, titer 4x109 Arrayed EST cDNA libraries
(constructed by Express Genomics, Inc.) Outbred adult thymus
Outbred adult splenocytes activated for 4 days with PHA X. tropicalis
cDNA libraries (λZAP-express) Trop: Adults spleen + thymus + intestine. Titer 5x1010