Bruker MuVi SPIM

Capabilities:

• Lightsheet Imaging of DBE and Aqueous cleared samples
• Lightsheet imaging of live samples

Summary of Instrument Features:

Microscope: Bruker Luxendo MuVi

Software: LuxControl

Octagons:

• LS Octagon 20x:
  Detection Objective - 20x/1.0 water Olympus
  Illumination Objective – 10x/0.3 water Nikon
  Compatibility – Aqueous Solutions Only
  Environmental Chamber Available
  Dual-sided Illumination and Detection
• CS Octagon 10x:
  Detection Objective - 10x/0.5 Nikon Multi-Imersion
  Illumination Objective – 10x/0.3 Nikon Air
  Compatibility – DBE, Aqueous
  Dual-sided Illumination and Single-sided Detection
• CS Octagon 4x:
  Detection Objective - 4x/0.28  Air Olympus
  Illumination Objective – 4x/0.2 Air Nikon
  Compatibility – DBE, Aqueous
  Dual-Sided Illumination and Single-sided Detection

Laser lines:

• Excitation:
  405nm diode
  445nm diode
  488nm diode
  515nm diode
  561nm diode
  642nm diode

Detectors: Hamamatsu Orca Flash 4.0 V3 sCMOS

Stage:  Motorized stage X, Y, Z, rotation

Environmental Chamber: Luxendo Control Unit with gas mixer

• Temperature control (18-40C)
• CO2
• Humidity Control