URMC / Research / Electron Microscopy
Welcome to the Electron Microscopy Shared Resource Laboratory
Routine Transmission and Scanning Electron Microscopy (TEM & SEM) Services
The goal of the Electron Microscopy Shared Resource Laboratory (EM-SRL) is to support investigators in their pursuit of studies involving electron microscopy at the lowest possible hourly fee.
Investigators can examine their research specimens on a Hitachi 7650 transmission electron microscope as the real-time digital image is displayed on a computer monitor. The size of each digital image captured is 7 MB and is saved as a TIFF or JPEG file. Each photography session’s digital files are saved and shared via URMC BOX.
The EM-SRL can also perform Scanning Electron Microscopy on any specimen type using a Zeiss Auriga SEM.
To arrange a consultation, please contact the Director, Karen_Bentley@urmc.rochester.edu or by phone at (585) 275-1954 (office).
Cryo-Electron Microscopy Services
With the installation of a FEI Talos 120C electron microscope in June of 2020, the electron microscopy core can now support cryo-electron microscopy projects.
We offer screening at the negative stain and initial vitrification stages for single particle analysis (SPA) as well as preliminary data collection on proteins and/or complexes (MW > ~150 kDa). Outside of SPA, vitrification can be utilized to view other specimens in a more native state. Examples include exosomes, virus-like particles (VLPs) or nano-materials (nano-particles and tubules) which have traditionally been imaged dehydrated following negative stain.
Samples are imaged with a 16 MP CMOS camera and can be acquired both manually or automated depending on the project.
To arrange a consultation, please contact the Cryo Facility Manager, Chad_Galloway@urmc.rochester.edu or by phone at (585) 275-9996 (office).
Lab fax: (585) 756-5337
Location: Medical School basement (elevators with yellow diamonds)
Intramural address: Pathology, Box 626