This instrument is currently disabled
Suggested voltages current as of Sept, 2019.
Detailed information about this instrument can be found on the baseline report (available upon request).
Reservations
Reservations can be made online by following this link.
You will need an approved USER account. Apply for one here.
To design a panel using Fluorofinder please click here.
LSR-II Configuration: BLUE 488 LASER (20mW): Detector | LP | Band Pass | Fluor's | Low Voltage | BLUE A | 735 | 780/60 | PE-Cy7, PE-Alexa750 | 500 | BLUE B | 685 | 710/50 | PerCP-Cy5.5, PE-Cy5.5, PerCP, PE-Alexa700 | 550 | BLUE C | 665 | 660/20 | PE-Cy5, PE-Alexa647, 7AAD, Fura-Low_Ca++ | 550 | BLUE D | 600 | 610/20 630/30 | PI, PE-Texas Red, PE-Alexa610, PE-Alexa594 | 500 | BLUE E | 550 | 576/26 | PE | 550 | BLUE F | 505 | 525/50 | FITC, Alexa488, YFP, GFP, CFSE | 400 | | BLUE G | | 488/10 | SSC | |
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RED 633 LASER (20mW): Detector | LP | Band Pass | Fluor's | Low Voltage | RED A | 735 | 780/60 | APC-Cy7, APC-Alexa750, APC-H7 | 450 | RED B | 685 | 710/50 | APC-Cy5.5, AlexaFluor 700 | 350 | RED C | | 660/20 | APC, Alexa 647 | 300 |
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VIOLET 405 LASER (40mW): Detector | LP | Band Pass | Fluor's | Low Voltage | VIOLET A | 595 | 610/20 | BV 605 | 400 | VIOLET B | 505 | 525/50 | Fura-High_Ca++, Pacific Orange | 350 | VIOLET C | | 450/50 | AlexaFluor 405, DAPI, BV 421, Pacific Blue | 300 |
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