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URMC / Research / Flow Cytometry / Instruments / Analytical / Rowlf

Rowlf Configuration - No longer Available (Oct 2017)

Suggested voltages current as of 11 March 2013.

LSRII Configuration: 

BLUE 488 LASER (20mW):

Detector

LP

Band Pass

Fluor's

 Suggested Voltage

BLUE A

735

780/60

PE-Cy7

 550

BLUE B

635

695/40

PerCP-Cy5.5, PE-Cy5.5, PE-Cy5, 7AAD, PerCP

 550

BLUE C

 550 585/42 PE 450

BLUE D

505

530/30

FITC, GFP, YFP, AlexaFluor 430, AlexaFluor 488, TO

 450

BLUE E

 

488/10

SSC

  

BLUE F

-

 

 

  
BLUE G - -    

BLUE H

---

-

 

 

RED 633 LASER (20mW):

Detector

LP

Band Pass

Fluor's

 Suggested Voltage

RED A

735

780/60

APC-Cy7

 550

RED B

 685 660/20 APC, Alexa 647, ToPro3 500

RED C

-

-

 

  

VIOLET 405 LASER (10mW):

Detector

LP

Band Pass

Fluor's

 Suggested Voltage

VIOLET A

505

535/50

Pacific Orange

 450

VIOLET B

 

450/50

Pacific Blue, DAPI, Hoechst 33258, Vibrant Violet

 350

VIOLET C

-

-