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URMC / Research / Rochester Genomics Center / Services / High Throughput Sequencing

 

High Throughput Sequencing

IlluminaKit+Sciclone

The GRC uses several kit-based workflows supplied by the following:

Illumina

  • TruSeq
  • Nextera

Agilent Technologies

  • SureSelect

Bioo Scientific

We also offer custom library preparations. Please contact us for more information.

Transcriptomics

Library Preparation Total RNA Input Target Capture Application
TruSeq Stranded mRNA-seq 200ng Poly-A RNA mRNA detection and differential expression, splice variants, rare transcripts complementary SNPs, captures both coding and non-coding transcripts that are poly adenylated, provides strand information, gene fusions
TruSeq Stranded Total RNA-seq 200ng Ribosomal RNA Depletion (RiboZero) coding and multiple forms of non-coding RNAs, mRNA detection and differential expression, splice variants, rare transcripts, complementary SNPs, gene fusions, lincRNAs, works well for low quality RNA samples and FFPE samples
Low Input
RNA-seq (Clontech SMARTer Technology)
1 ng Poly A RNA Low input preparation for high quality RNA.
mRNA detection and differential expression, splice variants, rare transcripts, complementary SNPs
NEB Small RNA 500ng 3' and 5' adaptor ligation of small RNA small regulatory RNAs, including micro RN

Genomics

Library Preparation DNA Input Target Capture Application
TruSeq DNA PCR-Free 1-2ug DNA Whole genome sequencing, SNP/InDel indentification, high GC-rich regions, promoters, and repetitive content, reduced library bias and gaps
Nextera XT 1ng DNA Whole genome sequencing, small genomes, amplicons larger than 500bp, Enzymatic Tagmentation
Illumina DNA Prep
(Nextera Flex)
100-500ng DNA Whole genome sequencing, SNP/InDel identification, gene fusions
Nextera Mate-Pair 1-4ug DNA Whole genome sequencing, De Novo sequencing, genome assemblies
Nextera Rapid Whole Exome 50ng DNA Exome sequencing, 37Mb or 62Mb (expanded) target baits, custom targets also available
Agilent SureSelect Whole Exome 4ug DNA Exome sequencing, 48.2mB+UTRs (version 7), other options available, custom targets also available

Amplicon

  • T-Cell Repertoire Sequencing
  • B-Cell Repertoire Sequencing
  • 16S Sequencing