URMC / Research / Rochester Genomics Center / Services / RNA/DNA Extractions

RNA and DNA Extractions

A banner of photos that contains several different Qiagen extraction kits. Each of the 4 sections shows a particular box (standard red or blue with Qiagen logo) in addition to the reagents and consumables organized around the box.

We have several kit options for extraction of RNA and/or DNA from cells, tissues, FFPE, and serum/plasma.

Extraction Type	Input	Kit Options
Total RNA	Cells Tissue Blood	Qiagen RNeasy Plus* (Mini & Micro)** Qiagen PAXgene TRIzol RNA Thermo Fisher Tempus Spin Zymo Direct-zol (Mini & Micro) MagMAX bead-based methods
Total & microRNA	Cells Tissue FFPE	Qiagen miRNeasy (Mini & FFPE) ThermoFisher mirVana miRNA Isolation Kit
DNA	Cells Tissue Blood	Qiagen DNeasy Blood and Tissue
DNA & RNA	Cells Tissue	Qiagen AllPrep DNA/RNA (Mini & Micro)* Qiagen All Prep DNA/RNA/miRNA Universal (includes microRNA) MagMAX bead-based extractions

*RNeasy PLUS kits are highly suggested due to the inclusion of gDNA removal columns
**Kit selection dependent on input

The GRC also offers several other extraction kits. Please send an email to our main URGenomics email if you would like to discuss other alternatives or extraction sample types.

DNase Treatment

If you are unable to use the RNeasy Plus kit with the gDNA eliminator column, we recommended doing a DNase treatment. At the GRC, we use the TURBO DNA-free kit to completely digest DNA in RNA samples with subsequent DNase enzyme removal. Below is a sample with gDNA contamination (increase in region between ribosomal peaks) prior to treatment and post DNase treatment.

DNase

Genomics Research Center Guidelines and Recommendations for submitting samples for extraction:

1. The GRC prefers the use of RLT Plus with the RNeasy Plus kits as this includes a gDNA eliminator column. The GRC is able to provide researchers who will submit samples for extraction with RLT Plus (1 mL RLT Plus and 10uL BME).
2. For sorted cells, do not exceed 1/3 volume of RLT Plus (100 uL cells per 350 uL RLT Plus). Vortex well before storing at -80C.
3. Please avoid resuspending in 15mL conicals.
4. If submitting cells, please indicate cell number on the submission sheet. If submitting tissue, please indicate tissue weight on the submission sheet. These factors will influence the kit that is used for extraction.
5. Please contact the GRC for more information about sample guidelines

QIAcube Connect MDx

QIAcube Connect MDx Instrument The GRC has the QIAcube Connect MDx for automation of extractions.

Visit the Qiagen QIAcube Connect MDx webpage for more information about the capabilities of the system.