Research Interest: We propose to develop primary mouse airway epithelial cell organoids. These will be infused with activated and memory CD8 OT-I T cells as an in vitro model of the airways to study motility. Organoids offer several advantages such as the ability to use genetically engineered epithelial cells as well as Tcells, they are optically transparent, and we seek to gather higher resolution imaging and fine measurements of T cell/EC cell behaviors related to motility that are difficult or impossible to obtain in vivo.