MIFLOWCYT information specific for this instrument can be found in the FCC_Library.
Detailed information about the QC can be found in the baseline report (available upon request).
Reservations can be made online by following this link.
You will need an approved USER account. Apply for one here.
To design a panel using Fluorofinder please click here.
Training Videos can be accessed here.
LSRII Configuration: Low starting voltages as of Jan 2023 posted at the machine and available via FCC_Library. BLUE 488 LASER (100mW): Detector | LP | Band Pass | Fluor's (check Fluorofinder for more info) | BLUE A | 685 | 710/50 | PerCP-Cy5.5 | BLUE B | 505 | 515/20 | FITC, CFSE, GFP, Alexa 488 | BLUE C | | 488/10 | SSC | FSC | | | |
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RED 633 LASER (40mW): Detector | LP | Band Pass | Fluor's (check Fluorofinder for more info) | RED A | 740 | 780/60 | APC-Cy7, APC-Alexa 750 | RED B | 685 | 710/50 | APC-Cy5.5, Alexa 700, Alexa 680 | RED C | | 660/20 | APC, Alexa 647 |
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VIOLET 407 LASER (35mW): Detector | LP | Band Pass | Fluor's (check Fluorofinder for more info) | VIOLET A | 740 | 780/60 | Qdot 800, BV 786 | VIOLET B | 670 | 705/70 | Qdot 705, BV 711 | VIOLET C | 630 | 660/40 | Qdot 655, BV 650 | | VIOLET D | 595 | 605/40 | Qdot 605, BV 605 | | VIOLET E | 570 | 585/42 | Qdot 585 | | VIOLET F | 557 | 560/40 | Qdot 565 | | VIOLET G | 535 | 550/40 | Pacific Orange, BV 510, Qdot 545 | | VIOLET H | | 450/50 | Pacific Blue, Alexa 405, BV 421 |
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GREEN 532 LASER (120mW): Detector | LP | Band Pass | Fluor's (check Fluorofinder for more info) | GREEN A | 740 | 780/40 | PE-Cy7, PE-Alexa 750 | GREEN B | 690 | 710/50 | PE-Cy5.5 | GREEN C | 640 | 660/40 | 7AAD, PE-Cy5 | GREEN D | 600 | 610/20 | PE-Alexa 610, PE-Texas Red | GREEN E | | 575/25 | PE, Alexa 532, PI |
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