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Oscar the Grouch Configuration

Suggested Voltage current as of Oct 2, 2017.

Detailed information about this instrument can be found in the baseline report (available upon request).

Reservations

Reservations can be made online by following this link.

You will need an approved USER account. Apply for one here.

To design a panel using Fluorofinder please click here.

LSR-II Configuration:

BLUE 488 LASER (20mW):

Detector

LP

Band Pass

Fluor's

Low Starting Voltage

BLUE A

685

710/50

PerCP-Cy5.5

600

BLUE B

505

515/20

FITC, CFSE, GFP, Alexa 488

550

BLUE C

  488/10 SSC  

FSC

 

 

 

 

RED 633 LASER (100mW):

Detector

LP

Band Pass

Fluor's

Low Starting Voltage

RED A

740

780/60

APC-Cy7, APC-Alexa 750

500

RED B

685 710/50 APC-Cy5.5, Alexa 700 400

RED C

 

660/20

APC, Alexa 647

500

VIOLET 405 LASER (100mW):

Detector

LP

Band Pass

Fluor's

Low Starting Voltage

VIOLET A

740

780/60

BV 786

600

VIOLET B

670

705/70

BV 711

650

VIOLET C

630

660/40

BV 650

500
VIOLET D 595 605/40 BV 605 550
VIOLET E 570 585/42 Qdot 585 450
VIOLET F 557 565/20 BV570 500
VIOLET G 535 550/40 BV 510, LiveDead Yellow 450
VIOLET H   450/50 Pacific Blue, BV 421 350

GREEN 532 LASER (120mW):

Detector

LP

Band Pass

Fluor's

Low Starting Voltage

GREEN A

740

780/40

PE-Cy7, PE-Alexa 750

550

GREEN B

690

710/50

PE-Cy5.5

500

GREEN C

640 660/40 7AAD, PE-Cy5 400

GREEN D

600

610/20

PE-Alexa 610, PE-Texas Red

400

GREEN E

 

575/25

PE,  PI

350