URMC / Rochester Human Immunology Center / Procedures

Procedures

To see a procedure, click on the Download link to view the PDF. If you have any questions about a procedure listed, or if you would like information about a procedure not available for download, please contact Sally Quataert.

Laboratory Methods

Procedure #	Title	File
HIC 1-0001.3	Immunofluorescence Staining for Multichromatic FACS Analysis with Human Single Cell Suspensions	Download Immunofluorescence Staining for Multichromatic FACS Analysis with Human Single Cell Suspensions
HIC 1-0002.2	Detection of Human Cytokine Response by Elispot in Human peripheral blood mononuclear cells following stimulation with antigen	Download Detection of Human Cytokine Response by Elispot in Human peripheral blood mononuclear cells following stimulation with antigen
HIC 1-0003.1	Detection of Multiple Human Cytokine responses by Fluorispot in Human peripheral blood mononuclear cells following stimulation with antigen	Download Detection of Multiple Human Cytokine responses by Fluorispot in Human peripheral blood mononuclear cells following stimulation with antigen
HIC 1-0004	Micro-immunofluorescence Staining for Multichromatic FACS Analysis with Human Single Cell Suspensions	Download Micro-immunofluorescence Staining for Multichromatic FACS Analysis with Human Single Cell Suspensions
HIC 1-0005	Proper Use and Handling of Staphylococcal Enterotoxin B (SEB).	Download Proper Use and Handling of Staphylococcal Enterotoxin B (SEB).
HIC 1-0006	Quality Control Procedure for antibody-fluorochrome conjugate reagents
HIC 1-0007.2	Cryopreservation of cells: Freezing and thawing	Download Cryopreservation of cells: Freezing and thawing
HIC 1-0008	Carboxyfluorescein Diacetate Succinimdyl Ester (CFDA SE) labeling of Lymphocytes for CFSE proliferation Studies	Download Carboxyfluorescein Diacetate Succinimdyl Ester (CFDA SE) labeling of Lymphocytes for CFSE proliferation Studies
HIC 1-0009	Detection of IgG and IgM Secreting B cells in human peripheral blood mononuclear cells by Elispot	Download Detection of IgG and IgM Secreting B cells in human peripheral blood mononuclear cells by Elispot
HIC 1-0010	Detection of anti-ds DNA specific IgG and IgM Secreting B cells in human peripheral blood mononuclear cells by Elispot
HIC 1-0011	RPB Program Phenotye Purity Check by immunofluorescent Staining following Magnetic bead isolation of cell populations
HIC 1-0012.2	RPB Processing of Clinical Specimens
HIC 1-0013	Negative or Positive Selection of a Specific Cell Population from Human PBMC utilizing magnetic cell sorting kit from Miltenyi Biotec	Download Negative or Positive Selection of a Specific Cell Population from Human PBMC utilizing magnetic cell sorting kit from Miltenyi Biotec
HIC-1-0014	Phenotye Purity Check by immunofluorescent Staining following Magnetic bead isolation of cell populations
HIC-1-0015	Generation of Epstein-Barr Virus (EBV) Immortalized B cell lines with human lymphocytes
HIC-1-0016	Intracellular Staining of human lymphoid cells for FoxP3 expression	Download Intracellular Staining of human lymphoid cells for FoxP3 expression
HIC-1-0017 draft	Intracellular Staining of human lymphoid cells
HIC-1-0018	Processing of Blood from Study C-935788-009 for Syk Signaling
HIC-1-0019	Isolation of Human Peripheral Blood Mononuclear Cells using BD CPT tube	Download Isolation of Human Peripheral Blood Mononuclear Cells using BD CPT tube
HIC-1-0020	Isolation of Human Peripheral Blood Mononuclear Cells using Ficoll-Hypaque Density Gradient Centrifugation	Download Isolation of Human Peripheral Blood Mononuclear Cells using Ficoll-Hypaque Density Gradient Centrifugation
HIC-1-0021	Immunofluorescence Staining for sorting of specific cell populations with Human Single Cell Suspensions.	Download Immunofluorescence Staining for sorting of specific cell populations with Human Single Cell Suspensions.
HIC-1-0022	Processing of Patient Samples for the ITN Oral Insulin Study ITN516AI (TN-07)

Quality Management

Procedural #	Title
HIC 3-0001	HIC Quality Management Program
HIC 3-0002	HIC Document Management Policy
HIC 3-0003	How to Write an Standard Operating Procedure
HIC 3-0004	HIC Records Retention and Management Policy
HIC 3-0005	HIC Equipment Management Program
HIC 3-0006	HIC Equipment Qualification program
HIC 3-0007	HIC Training policy
HIC 3-0008	HIC Laboratory Safety policy
HIC 3-0009	HIC Materials Management policy
HIC 3-0010	Reagent Quality Control and Inventory Policy
HIC 3-0011	Antibody-Fluorochrome Management Policy
HIC 3-0012	HIC Standard and Control Reagent Program
HIC 3-0013	HIC Change Control Program
HIC 3-0014	Bioanalytical Method Validation Program
HIC 3-0015	Scientific notebook policy
HIC 3-0016	Technical and Clinical Report Policy
HIC 3-0017	Validation Plan, Protocol and Report Policy
HIC 3-0018	Quality Control Database Management
HIC 3-0019	HIC Corrective and Preventative Action Plan Program
HIC 3-0020	Computer System Validation
HIC 3-0021	Database management policy
HIC 3-0022	HIC Website Management policy
HIC 3-0023	Clinical Specimen Management Policy
HIC 3-0024	Clinical Study Management Policy
HIC 3-0025	Clinical Specimen Transport, Shipping, Receipt, and Distribution
HIC 3-0026	HIPAA Confidentiality Policy
HIC 3-0027	HIC Retest Policy for Clinical testing
HIC 3-0028	HIC Laboratory Investigation Policy
HIC 3-0029	HIC Phlebotomy Service Policy
HIC 3-0030	International Shipping Procedure for Clinical Specimens

Facilities and Equipment Management

Procedure #	Title	File
HIC 4-0001	Centrifuge Maintenance/Calibration/Cleaning SOP	Download Centrifuge Maintenance/Calibration/Cleaning SOP
HIC 4-0002	Plate Washer Maintenance/Calibration/Cleaning SOP
HIC 4-0003	Operation and Maintenance of the VWR Model 1200 Water Bath	Download Operation and Maintenance of the VWR Model 1200 Water Bath
HIC 4-0004	Operation and Maintenance of the Binder APT Line CB CO2 Incubator	Download Operation and Maintenance of the Binder APT Line CB CO2 Incubator
HIC 4-0005	Operation of Fyrite Apparatus for monitoring CO2
HIC 4-0006	Operation and Maintenance of the Biological Safety Cabinet	Download Operation and Maintenance of the Biological Safety Cabinet
HIC 4-0007	Operation and Maintenance of the Traceable Memory Monitoring Thermometer	Download Operation and Maintenance of the Traceable Memory Monitoring Thermometer
HIC 4-0008	Operation and Maintenance of the -80ºC Freezer	Download Operation and Maintenance of the -80ºC Freezer
HIC 4-0009	Operation and Maintenance of the VWR VistaVision Inverted Compound Microscope Model 12778-198	Download Operation and Maintenance of the VWR VistaVision Inverted Compound Microscope Model 12778-198
HIC 4-0010	CryoMed Maintenance/Calibration/Cleaning SOP	Download CryoMed Maintenance/Calibration/Cleaning SOP
HIC 4-0011	Balance Maintenance/Calibration/Cleaning SOP	Download Balance Maintenance/Calibration/Cleaning SOP
HIC 4-0012	Operation and Maintenance of the VWR Model SB20 Bench-top pH Meter	Download Operation and Maintenance of the VWR Model SB20 Bench-top pH Meter
HIC 4-0013	CTL Reader Maintenance/Calibration/Cleaning SOP
HIC 4-0014	LSRII Maintenance/Calibration/Cleaning SOPs
HIC 4-0015	LSRII Administration
HIC 4-0016	LSRII daily Quality control testing
HIC 4-0017	Operation and Maintenance of the Refrigerator	Download Operation and Maintenance of the Refrigerator
HIC 4-0018	Pipet Maintenance/Calibration/Cleaning SOP	Download Pipet Maintenance/Calibration/Cleaning SOP
HIC 4-0019	Server Maintenance SOP
HIC 4-0020	Immunofluorescence Microscop Maintenance/Calibration/Cleaning SOP
HIC 4-0021	Operation of a BSLII Laboratory
HIC 4-0022	Thermocouple Use/Maintenance/Calibration SOP
HIC 4-0023	Operation of the BD LSRII Flow Cytometer and HTS sampler attachment
HIC 4-0024	Operation and maintenance of laboratory eyewash stations	Download Operation and maintenance of laboratory eyewash stations

Training Manuals

Procedure #	Title
HIC 5-0001	BD LSRII User Guide Version 1.0
HIC 5-0002	HIC LSRII Guidelines