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Procedures

To see a procedure, click on the Download link to view the PDF. If you have any questions about a procedure listed, or if you would like information about a procedure not available for download, please contact Sally Quataert.

Laboratory Methods

Procedure # Title File
HIC 1-0001.3 Immunofluorescence Staining for Multichromatic FACS Analysis with Human Single Cell Suspensions Download
HIC 1-0002.2 Detection of Human Cytokine Response by Elispot in Human peripheral blood mononuclear cells following stimulation with antigen Download
HIC 1-0003.1 Detection of Multiple Human Cytokine responses by Fluorispot in Human peripheral blood mononuclear cells following stimulation with antigen Download
HIC 1-0004 Micro-immunofluorescence Staining for Multichromatic FACS Analysis with Human Single Cell Suspensions Download
HIC 1-0005 Proper Use and Handling of Staphylococcal Enterotoxin B (SEB). Download
HIC 1-0006 Quality Control Procedure for antibody-fluorochrome conjugate reagents  
HIC 1-0007.2 Cryopreservation of cells: Freezing and thawing Download
HIC 1-0008 Carboxyfluorescein Diacetate Succinimdyl Ester (CFDA SE) labeling of Lymphocytes for CFSE proliferation Studies Download
HIC 1-0009 Detection of IgG and IgM Secreting B cells in human peripheral blood mononuclear cells by Elispot Download
HIC 1-0010 Detection of anti-ds DNA specific IgG and IgM Secreting B cells in human peripheral blood mononuclear cells by Elispot  
HIC 1-0011 RPB Program Phenotye Purity Check by immunofluorescent Staining following Magnetic bead isolation of cell populations  
HIC 1-0012.2 RPB Processing of Clinical Specimens  
HIC 1-0013 Negative or Positive Selection of a Specific Cell Polulation from Human PBMC utilizing magnetic cell sorting kit from Miltenyi Biotec Download
HIC-1-0014 Phenotye Purity Check by immunofluorescent Staining following Magnetic bead isolation of cell populations  
HIC-1-0015 Generation of Epstein-Barr Virus (EBV) Immortalized B cell lines with human lymphocytes  
HIC-1-0016 Intracellular Staining of human lymphoid cells for FoxP3 expression Download
HIC-1-0017 draft Intracellular Staining of human lymphoid cells  
HIC-1-0018 Processing of Blood from Study C-935788-009 for Syk Signaling  
HIC-1-0019 Isolation of Human Peripheral Blood Mononuclear Cells using BD CPT tube Download
HIC-1-0020 Isolation of Human Peripheral Blood Mononuclear Cells using Ficoll-Hypaque Density Gradient Centrifugation Download
HIC-1-0021 Immunofluorescence Staining for sorting of specific cell populations with Human Single Cell Suspensions. Download
HIC-1-0022 Processing of Patient Samples for the ITN Oral Insulin Study ITN516AI (TN-07)  

Quality Management

Procedurel # Title
HIC 3-0001 HIC Quality Management Program
HIC 3-0002 HIC Document Management Policy
HIC 3-0003 How to Write an Standard Operating Procedure
HIC 3-0004 HIC Records Retention and Management Policy
HIC 3-0005 HIC Equipment Management Program
HIC 3-0006 HIC Equipment Qualification program
HIC 3-0007 HIC Training policy
HIC 3-0008 HIC Laboratory Safety policy
HIC 3-0009 HIC Materials Management policy
HIC 3-0010 Reagent Quality Control and Inventory Policy
HIC 3-0011 Antibody-FluorochromeManagement Policy
HIC 3-0012 HIC Standard and Control Reagent Program
HIC 3-0013 HIC Change Control Program
HIC 3-0014 Bioanalytical Method Validation Program
HIC 3-0015 Scientific notebook policy
HIC 3-0016 Technical and Clinical Report Policy
HIC 3-0017 Validation Plan, Protocol and Report Poiicy
HIC 3-0018 Quality Control Database Management
HIC 3-0019 HIC Corrective and Preventative Action Plan Program
HIC 3-0020 Computer System Validation
HIC 3-0021 Database management policy
HIC 3-0022 HIC Website Management policy
HIC 3-0023 Clinical Specimen Management Policy
HIC 3-0024 Clinical Study Management Policy
HIC 3-0025 Clinical Specimen Transport, Shipping, Receipt, and Distribution
HIC 3-0026 HIPAA Confidentiality Policy
HIC 3-0027 HIC Retest Policy for Clinical testing
HIC 3-0028 HIC Laboratory Investigation Policy
HIC 3-0029 HIC Phlebotomy Service Policy
HIC 3-0030 International Shipping Procedure for Clinical Specimens

Facilities and Equipment Management

Procedure # Title File
HIC 4-0001 Centrifuge Maintenance/Calibration/Cleaning SOP Download
HIC 4-0002 Plate Washer Maintenance/Calibration/Cleaning SOP  
HIC 4-0003 Operation and Maintenance of the VWR Model 1200 Water Bath Download
HIC 4-0004 Operation and Maintenance of the Binder APT Line CB CO2 Incubator Download
HIC 4-0005 Operation of Fyrite Apparatus for monitoring CO2  
HIC 4-0006 Operation and Maintenance of the Biological Safety Cabinet Download
HIC 4-0007 Operation and Maintenance of the Traceable Memory Monitoring Thermometer Download
HIC 4-0008 Operation and Maintenance of the -80ºC Freezer Download
HIC 4-0009 Operation and Maintenance of the VWR VistaVision Inverted Compound Microscope Model 12778-198 Download
HIC 4-0010 CryoMed Maintenance/Calibration/Cleaning SOP Download
HIC 4-0011 Balance Maintenance/Calibration/Cleaning SOP Download
HIC 4-0012 Operation and Maintenance of the VWR Model SB20 Bench-top pH Meter Download
HIC 4-0013 CTL Reader Maintenance/Calibration/Cleaning SOP  
HIC 4-0014 LSRII Maintenance/Calibration/Cleaning SOPs  
HIC 4-0015 LSRII Administration   
HIC 4-0016 LSRII daily Quality control testing  
HIC 4-0017 Operation and Maintenance of the Refrigerator Download
HIC 4-0018 Pipet Maintenance/Calibration/Cleaning SOP Download
HIC 4-0019 Server Maintenance SOP  
HIC 4-0020 Immunofluorescence Microscop Maintenance/Calibration/Cleaning SOP  
HIC 4-0021 Operation of a BSLII Laboratory  
HIC 4-0022 Thermocouple Use/Maintenance/Calibration SOP  
HIC 4-0023 Operation of the BD LSRII Flow Cytometer and HTS sampler attachment  
HIC 4-0024 Operation and maintenance of laboratory eyewash stations Download

Training Manuals

Procedure # Title
HIC 5-0001 BD LSRII User Guide Version 1.0
HIC 5-0002 HIC LSRII Guidelines