URMC / Rochester Human Immunology Center / Procedures

Procedures

To see a procedure, click on the Download link to view the PDF. If you have any questions about a procedure listed, or if you would like information about a procedure not available for download, please contact Sally Quataert.

Laboratory Methods

Procedure #	Title	File
HIC 1-0001.3	Immunofluorescence Staining for Multichromatic FACS Analysis with Human Single Cell Suspensions	Download
HIC 1-0002.2	Detection of Human Cytokine Response by Elispot in Human peripheral blood mononuclear cells following stimulation with antigen	Download
HIC 1-0003.1	Detection of Multiple Human Cytokine responses by Fluorispot in Human peripheral blood mononuclear cells following stimulation with antigen	Download
HIC 1-0004	Micro-immunofluorescence Staining for Multichromatic FACS Analysis with Human Single Cell Suspensions	Download
HIC 1-0005	Proper Use and Handling of Staphylococcal Enterotoxin B (SEB).	Download
HIC 1-0006	Quality Control Procedure for antibody-fluorochrome conjugate reagents
HIC 1-0007.2	Cryopreservation of cells: Freezing and thawing	Download
HIC 1-0008	Carboxyfluorescein Diacetate Succinimdyl Ester (CFDA SE) labeling of Lymphocytes for CFSE proliferation Studies	Download
HIC 1-0009	Detection of IgG and IgM Secreting B cells in human peripheral blood mononuclear cells by Elispot	Download
HIC 1-0010	Detection of anti-ds DNA specific IgG and IgM Secreting B cells in human peripheral blood mononuclear cells by Elispot
HIC 1-0011	RPB Program Phenotye Purity Check by immunofluorescent Staining following Magnetic bead isolation of cell populations
HIC 1-0012.2	RPB Processing of Clinical Specimens
HIC 1-0013	Negative or Positive Selection of a Specific Cell Polulation from Human PBMC utilizing magnetic cell sorting kit from Miltenyi Biotec	Download
HIC-1-0014	Phenotye Purity Check by immunofluorescent Staining following Magnetic bead isolation of cell populations
HIC-1-0015	Generation of Epstein-Barr Virus (EBV) Immortalized B cell lines with human lymphocytes
HIC-1-0016	Intracellular Staining of human lymphoid cells for FoxP3 expression	Download
HIC-1-0017 draft	Intracellular Staining of human lymphoid cells
HIC-1-0018	Processing of Blood from Study C-935788-009 for Syk Signaling
HIC-1-0019	Isolation of Human Peripheral Blood Mononuclear Cells using BD CPT tube	Download
HIC-1-0020	Isolation of Human Peripheral Blood Mononuclear Cells using Ficoll-Hypaque Density Gradient Centrifugation	Download
HIC-1-0021	Immunofluorescence Staining for sorting of specific cell populations with Human Single Cell Suspensions.	Download
HIC-1-0022	Processing of Patient Samples for the ITN Oral Insulin Study ITN516AI (TN-07)

Quality Management

Procedurel #	Title
HIC 3-0001	HIC Quality Management Program
HIC 3-0002	HIC Document Management Policy
HIC 3-0003	How to Write an Standard Operating Procedure
HIC 3-0004	HIC Records Retention and Management Policy
HIC 3-0005	HIC Equipment Management Program
HIC 3-0006	HIC Equipment Qualification program
HIC 3-0007	HIC Training policy
HIC 3-0008	HIC Laboratory Safety policy
HIC 3-0009	HIC Materials Management policy
HIC 3-0010	Reagent Quality Control and Inventory Policy
HIC 3-0011	Antibody-FluorochromeManagement Policy
HIC 3-0012	HIC Standard and Control Reagent Program
HIC 3-0013	HIC Change Control Program
HIC 3-0014	Bioanalytical Method Validation Program
HIC 3-0015	Scientific notebook policy
HIC 3-0016	Technical and Clinical Report Policy
HIC 3-0017	Validation Plan, Protocol and Report Poiicy
HIC 3-0018	Quality Control Database Management
HIC 3-0019	HIC Corrective and Preventative Action Plan Program
HIC 3-0020	Computer System Validation
HIC 3-0021	Database management policy
HIC 3-0022	HIC Website Management policy
HIC 3-0023	Clinical Specimen Management Policy
HIC 3-0024	Clinical Study Management Policy
HIC 3-0025	Clinical Specimen Transport, Shipping, Receipt, and Distribution
HIC 3-0026	HIPAA Confidentiality Policy
HIC 3-0027	HIC Retest Policy for Clinical testing
HIC 3-0028	HIC Laboratory Investigation Policy
HIC 3-0029	HIC Phlebotomy Service Policy
HIC 3-0030	International Shipping Procedure for Clinical Specimens

Facilities and Equipment Management

Procedure #	Title	File
HIC 4-0001	Centrifuge Maintenance/Calibration/Cleaning SOP	Download
HIC 4-0002	Plate Washer Maintenance/Calibration/Cleaning SOP
HIC 4-0003	Operation and Maintenance of the VWR Model 1200 Water Bath	Download
HIC 4-0004	Operation and Maintenance of the Binder APT Line CB CO2 Incubator	Download
HIC 4-0005	Operation of Fyrite Apparatus for monitoring CO2
HIC 4-0006	Operation and Maintenance of the Biological Safety Cabinet	Download
HIC 4-0007	Operation and Maintenance of the Traceable Memory Monitoring Thermometer	Download
HIC 4-0008	Operation and Maintenance of the -80ºC Freezer	Download
HIC 4-0009	Operation and Maintenance of the VWR VistaVision Inverted Compound Microscope Model 12778-198	Download
HIC 4-0010	CryoMed Maintenance/Calibration/Cleaning SOP	Download
HIC 4-0011	Balance Maintenance/Calibration/Cleaning SOP	Download
HIC 4-0012	Operation and Maintenance of the VWR Model SB20 Bench-top pH Meter	Download
HIC 4-0013	CTL Reader Maintenance/Calibration/Cleaning SOP
HIC 4-0014	LSRII Maintenance/Calibration/Cleaning SOPs
HIC 4-0015	LSRII Administration
HIC 4-0016	LSRII daily Quality control testing
HIC 4-0017	Operation and Maintenance of the Refrigerator	Download
HIC 4-0018	Pipet Maintenance/Calibration/Cleaning SOP	Download
HIC 4-0019	Server Maintenance SOP
HIC 4-0020	Immunofluorescence Microscop Maintenance/Calibration/Cleaning SOP
HIC 4-0021	Operation of a BSLII Laboratory
HIC 4-0022	Thermocouple Use/Maintenance/Calibration SOP
HIC 4-0023	Operation of the BD LSRII Flow Cytometer and HTS sampler attachment
HIC 4-0024	Operation and maintenance of laboratory eyewash stations	Download

Training Manuals

Procedure #	Title
HIC 5-0001	BD LSRII User Guide Version 1.0
HIC 5-0002	HIC LSRII Guidelines