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Procedures

To see a procedure, click on the Download link to view the PDF. If you have any questions about a procedure listed, or if you would like information about a procedure not available for download, please contact Sally Quataert.

Laboratory Methods

Procedure # Title File
HIC 1-0001.3 Immunofluorescence Staining for Multichromatic FACS Analysis with Human Single Cell Suspensions Download Immunofluorescence Staining for Multichromatic FACS Analysis with Human Single Cell Suspensions
HIC 1-0002.2 Detection of Human Cytokine Response by Elispot in Human peripheral blood mononuclear cells following stimulation with antigen Download Detection of Human Cytokine Response by Elispot in Human peripheral blood mononuclear cells following stimulation with antigen
HIC 1-0003.1 Detection of Multiple Human Cytokine responses by Fluorispot in Human peripheral blood mononuclear cells following stimulation with antigen Download Detection of Multiple Human Cytokine responses by Fluorispot in Human peripheral blood mononuclear cells following stimulation with antigen
HIC 1-0004 Micro-immunofluorescence Staining for Multichromatic FACS Analysis with Human Single Cell Suspensions Download Micro-immunofluorescence Staining for Multichromatic FACS Analysis with Human Single Cell Suspensions
HIC 1-0005 Proper Use and Handling of Staphylococcal Enterotoxin B (SEB). Download Proper Use and Handling of Staphylococcal Enterotoxin B (SEB).
HIC 1-0006 Quality Control Procedure for antibody-fluorochrome conjugate reagents  
HIC 1-0007.2 Cryopreservation of cells: Freezing and thawing Download Cryopreservation of cells: Freezing and thawing
HIC 1-0008 Carboxyfluorescein Diacetate Succinimdyl Ester (CFDA SE) labeling of Lymphocytes for CFSE proliferation Studies Download Carboxyfluorescein Diacetate Succinimdyl Ester (CFDA SE) labeling of Lymphocytes for CFSE proliferation Studies
HIC 1-0009 Detection of IgG and IgM Secreting B cells in human peripheral blood mononuclear cells by Elispot Download Detection of IgG and IgM Secreting B cells in human peripheral blood mononuclear cells by Elispot
HIC 1-0010 Detection of anti-ds DNA specific IgG and IgM Secreting B cells in human peripheral blood mononuclear cells by Elispot  
HIC 1-0011 RPB Program Phenotye Purity Check by immunofluorescent Staining following Magnetic bead isolation of cell populations  
HIC 1-0012.2 RPB Processing of Clinical Specimens  
HIC 1-0013 Negative or Positive Selection of a Specific Cell Population from Human PBMC utilizing magnetic cell sorting kit from Miltenyi Biotec Download Negative or Positive Selection of a Specific Cell Population from Human PBMC utilizing magnetic cell sorting kit from Miltenyi Biotec
HIC-1-0014 Phenotye Purity Check by immunofluorescent Staining following Magnetic bead isolation of cell populations  
HIC-1-0015 Generation of Epstein-Barr Virus (EBV) Immortalized B cell lines with human lymphocytes  
HIC-1-0016 Intracellular Staining of human lymphoid cells for FoxP3 expression Download Intracellular Staining of human lymphoid cells for FoxP3 expression
HIC-1-0017 draft Intracellular Staining of human lymphoid cells  
HIC-1-0018 Processing of Blood from Study C-935788-009 for Syk Signaling  
HIC-1-0019 Isolation of Human Peripheral Blood Mononuclear Cells using BD CPT tube Download Isolation of Human Peripheral Blood Mononuclear Cells using BD CPT tube
HIC-1-0020 Isolation of Human Peripheral Blood Mononuclear Cells using Ficoll-Hypaque Density Gradient Centrifugation Download Isolation of Human Peripheral Blood Mononuclear Cells using Ficoll-Hypaque Density Gradient Centrifugation
HIC-1-0021 Immunofluorescence Staining for sorting of specific cell populations with Human Single Cell Suspensions. Download Immunofluorescence Staining for sorting of specific cell populations with Human Single Cell Suspensions.
HIC-1-0022 Processing of Patient Samples for the ITN Oral Insulin Study ITN516AI (TN-07)  

Quality Management

Procedural # Title
HIC 3-0001 HIC Quality Management Program
HIC 3-0002 HIC Document Management Policy
HIC 3-0003 How to Write an Standard Operating Procedure
HIC 3-0004 HIC Records Retention and Management Policy
HIC 3-0005 HIC Equipment Management Program
HIC 3-0006 HIC Equipment Qualification program
HIC 3-0007 HIC Training policy
HIC 3-0008 HIC Laboratory Safety policy
HIC 3-0009 HIC Materials Management policy
HIC 3-0010 Reagent Quality Control and Inventory Policy
HIC 3-0011 Antibody-Fluorochrome Management Policy
HIC 3-0012 HIC Standard and Control Reagent Program
HIC 3-0013 HIC Change Control Program
HIC 3-0014 Bioanalytical Method Validation Program
HIC 3-0015 Scientific notebook policy
HIC 3-0016 Technical and Clinical Report Policy
HIC 3-0017 Validation Plan, Protocol and Report Policy
HIC 3-0018 Quality Control Database Management
HIC 3-0019 HIC Corrective and Preventative Action Plan Program
HIC 3-0020 Computer System Validation
HIC 3-0021 Database management policy
HIC 3-0022 HIC Website Management policy
HIC 3-0023 Clinical Specimen Management Policy
HIC 3-0024 Clinical Study Management Policy
HIC 3-0025 Clinical Specimen Transport, Shipping, Receipt, and Distribution
HIC 3-0026 HIPAA Confidentiality Policy
HIC 3-0027 HIC Retest Policy for Clinical testing
HIC 3-0028 HIC Laboratory Investigation Policy
HIC 3-0029 HIC Phlebotomy Service Policy
HIC 3-0030 International Shipping Procedure for Clinical Specimens

Facilities and Equipment Management

Procedure # Title File
HIC 4-0001 Centrifuge Maintenance/Calibration/Cleaning SOP Download Centrifuge Maintenance/Calibration/Cleaning SOP
HIC 4-0002 Plate Washer Maintenance/Calibration/Cleaning SOP  
HIC 4-0003 Operation and Maintenance of the VWR Model 1200 Water Bath Download Operation and Maintenance of the VWR Model 1200 Water Bath
HIC 4-0004 Operation and Maintenance of the Binder APT Line CB CO2 Incubator Download Operation and Maintenance of the Binder APT Line CB CO2 Incubator
HIC 4-0005 Operation of Fyrite Apparatus for monitoring CO2  
HIC 4-0006 Operation and Maintenance of the Biological Safety Cabinet Download Operation and Maintenance of the Biological Safety Cabinet
HIC 4-0007 Operation and Maintenance of the Traceable Memory Monitoring Thermometer Download Operation and Maintenance of the Traceable Memory Monitoring Thermometer
HIC 4-0008 Operation and Maintenance of the -80ºC Freezer Download Operation and Maintenance of the -80ºC Freezer
HIC 4-0009 Operation and Maintenance of the VWR VistaVision Inverted Compound Microscope Model 12778-198 Download Operation and Maintenance of the VWR VistaVision Inverted Compound Microscope Model 12778-198
HIC 4-0010 CryoMed Maintenance/Calibration/Cleaning SOP Download CryoMed Maintenance/Calibration/Cleaning SOP
HIC 4-0011 Balance Maintenance/Calibration/Cleaning SOP Download Balance Maintenance/Calibration/Cleaning SOP
HIC 4-0012 Operation and Maintenance of the VWR Model SB20 Bench-top pH Meter Download Operation and Maintenance of the VWR Model SB20 Bench-top pH Meter
HIC 4-0013 CTL Reader Maintenance/Calibration/Cleaning SOP  
HIC 4-0014 LSRII Maintenance/Calibration/Cleaning SOPs  
HIC 4-0015 LSRII Administration   
HIC 4-0016 LSRII daily Quality control testing  
HIC 4-0017 Operation and Maintenance of the Refrigerator Download Operation and Maintenance of the Refrigerator
HIC 4-0018 Pipet Maintenance/Calibration/Cleaning SOP Download Pipet Maintenance/Calibration/Cleaning SOP
HIC 4-0019 Server Maintenance SOP  
HIC 4-0020 Immunofluorescence Microscop Maintenance/Calibration/Cleaning SOP  
HIC 4-0021 Operation of a BSLII Laboratory  
HIC 4-0022 Thermocouple Use/Maintenance/Calibration SOP  
HIC 4-0023 Operation of the BD LSRII Flow Cytometer and HTS sampler attachment  
HIC 4-0024 Operation and maintenance of laboratory eyewash stations Download Operation and maintenance of laboratory eyewash stations

Training Manuals

Procedure # Title
HIC 5-0001 BD LSRII User Guide Version 1.0
HIC 5-0002 HIC LSRII Guidelines