The histology core's mission is to provide processing, embedding, sectioning and staining of animal or human tissues in a timely manner. According to the goal of projects and the animal models of human diseases, the core also provide customizing assistance to obtain high quality data and the training for CVRI personnel using histology equipment.
Core instrumentation includes:
- Leica Autostainer XL
- Leica Cryostat CM 1950; Sakura Tissue-Tek TEC 5
- Leica Microtome RM 2255
- Leica Processor TP 1020
- Olympus microscope BX41TF.
CVRI MICROSCOPY Core: SUMMARY OF CAPABILITIES
The CVRI Microscopy Core consists of 5 well-maintained microscopes. The Core is overseen by Drs. Chen Yan and Linda Callahan. The Microscopy Core works in conjunction with the CVRI Histology core and the microsurgery core for design and interpretation of microscopy-related experiments. The 5 microscopes within the core include a ZOE imaging system (ZOE), an upright epifluorescence microscope (BX51), an inverted epifluorescence microscope (IX 70), a timelapse/FRET microscope capable of FRET, multipoint timelapse imaging, and TIRF, and a laser point scanning confocal microscope (FV 1000 confocal). Dr. Linda Callahan trains all users on each of the microscopes and assists with trouble shooting of experiments and experimental design. Dr. Callahan also maintains the microscopes, working with vendors as needed to upgrade or maintain instruments. Below is a brief summary of the capabilities of each microscope.
- The ZOE imaging system allows for quick observation of cell cultures and slides. Best used for checking progress of culture expression or slide staining. Not for high resolution work, but excellent for quick checks of staining or expression progress.
- The BX51 microscope is capable of standard brightfield and epifluorescence microscopy, polarized light microscopy, phase contrast and differential interference microscopy. Full complement of objectives and a DP74 high resolution camera. Mercury lamp excitation and epifluorescence capabilities for blue to near red emitting fluorophores. Software includes measurement, post-processing, and ‘smart montage’ capabilities. Original meta-data with each image. It is in room G.11354.
- The IX70 microscope is capable of observation of cell culture and slides. Standard brightfield and epifluorescence. Mercury lamp excitation. It is in room G.11352.
- The timelapse/FRET microscope is capable of FRET, long-term muti-point timelapse, and TIRF imaging. Easily images live cell culture using glass bottom coverslip dishes or fixed tissue/fixed cultures. Fully automated. Inverted microscope with an enclosed temperature, humidity, and CO2 environmental control chamber. Multiple filter combination capabilities. Xenon lamp excitation and Hamamatsu Orca camera. It is in room G11006.
- The FV1000 confocal microscope is a laser point scanning confocal microscope with high resolution imaging capabilities including 0.4 um slice capabilities using the 60x NA 1.42 objective. Applications include 2-4 channel 2D and 3D imaging, FRAP, FRET, timelapse, measurement, and post-processing capabilities. Original meta-data with each image. 6 laser lines (405, 440, 488, 515, 559, 635) enable imaging fluorophores emitting in the blue to far red spectrum. Floating table, in-room lighting adjustment capabilities, and secure one-way movement of images from the imaging computer to the CVRI confocal microscope server. Server is secured and designed to give PI full control over their data. It is in room G.11008.
Optimal imaging for the high resolution microscopes is best when tissue samples or cultures are imaged through 0.17 mm coverslips or 0.17 mm glass bottom dishes (#1.5 coverslips in catalogs). For more information, please contact Chen_Yan@urmc.rochester.edu and Linda_Callahan@urmc.rochester.edu.
The goal of the Microsurgical and Cardiovascular Pathophysiology Core is to support small animal pre-clinical translational and mechanistic research conducted by Aab CVRI investigators. The Core is staffed by three full time employees who provide a wide range of services, including microsurgeries, ultrasound imaging, invasive hemodynamics, and precision tissue harvesting. We are also happy to work with investigators to develop new customized surgical models of cardiovascular disease.
- Support Staff and Contact
- Microsurgical models of Cardiovascular Remodeling
- Customized Surgery development
- Cardiovascular physiology