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URMC / Cardiovascular Research Institute / Research / Yan Lab / Model Systems and Techniques

 

Model Systems and Techniques

We employ several model systems and techniques in our laboratory including the following:

Model Systems

  • Langendorff perfused mouse hearts (Picture 1)
  • Isolation and culture of adult mouse cardiomyocytes
  • Transverse aortic constriction induced mouse heart failure model
  • Carotid artery partial ligation mouse model
  • Femoral artery wire injury mouse model
  • Abdominal aortic aneurysms mouse model
  • Atherosclerosis mouse model
perfusion system photo

Picture 1. Langendorff heart perfusion system

Molecular, Biochemical, and Cell Biological Techniques

  • Molecular cloning
  • Western blotting
  • Immunoprecipitation
  • Immunostaining
  • RT-PCR
  • Transgenic gene expression
  • Lentivirus/Adenovirus/Adeno-associated viruses-mediated gene delivery

Optical/Imaging Techniques

  • Fluorescence energy resonance transfer (Picture 2)
  • Confocal imaging
  • Electron microscopy
rat fibroblasts

Picture 2. Representative image of primary rat cardiac fibroblasts transfected with cAMP FRET sensor Epac1-camps. Pseudo-colored fluorescence intensity is shown for the FRET donor, CFP (485 nm), the acceptor YFP (535 nm), corrected FRET ratio 485/535 nm.

Physiological Assays

  • In vitro cardiomyocyte contraction (Picture 3)
  • Ex vivo assessment of cardiac physiology
  • Echocardiographic evaluation of cardiac function
ionoptix system

Picture 3. IonOptix Calcium and Contractility system for cardiomyocyte contractile function.