Structural Biology & Biophysics Facility
The Structural Biology and Biophysics Facility is a University of Rochester research resource located within the Department of Biochemistry & Biophysics. Our goal is to provide non-specialist and specialist users with access to biophysical instrumentation designed to:
Learn more about our Services and User Fees.
The facility features the following equipment:
- Bruker AXS X8 Prospector Ultra microfocus IµS sealed-tube X-ray generator
- Mosquito crystallization robot (TTP LabTech) or high-throughput, nanoliter-scale crystallization experiments
- Microscopes for crystal viewing
- Computers and software for structure determination and graphics
- Dynamic Light Scattering (DLS) DynaPro Plate Reader II (Wyatt Technologies)
- BIAcore T200 for automated surface plasmon resonance (SPR)
- VP-ITC (Microcal) isothermal titration calorimeter
- Fluoromax-3 fluorimeter
- ÄKTA Pure modular chromatography system
- JASCO J-1100 Circular Dichroism (CD) spectrometer
- Avestin EmulsiFlex-C3 Homogenizer
Dr. Jermaine Jenkins is the Facility Manager who is available to train individuals in the use of core instruments. He can also assist with experimental design and data interpretation. Dr. Jenkins reports to the Executive Directors of the facility, Profs. Joseph Wedekind and Clara Kielkopf. Operation of the Facility is overseen by a steering committee comprised of local scientists.
Recent Facility Publications
- Talkish J, Igel H, Hunter O, Horner SW, Jeffery NN, Leach JR, Jenkins JL, Kielkopf CL, Ares M Jr. Cus2 enforces the first ATP-dependent step of splicing by binding to yeast SF3b1 through a UHM-ULM interaction. RNA. 2019 May 20;. doi: 10.1261/rna.070649.119.
- Hilt ZT, Pariser DN, Ture SK, Mohan A, Quijada P, Asante AA, Cameron SJ, Sterling JA, Merkel AR, Johanson AL, Jenkins JL, Small EM, McGrath KE, Palis J, Elliott MR, Morrell CN. Platelet-derived β2M regulates monocyte inflammatory responses. JCI Insight. 2019 Mar 7;4(5). pii: 122943. doi: 10.1172/jci.insight.122943. eCollection 2019 Mar 7.
- Starr ML, Sparks RP, Arango AS, Hurst LR, Zhao Z, Lihan M, Jenkins JL, Tajkhorshid E, Fratti RA. Phosphatidic acid induces conformational changes in Sec18 protomers that prevent SNARE priming. J Biol Chem. 2019 Mar 1;294(9):3100-3116. doi: 10.1074/jbc.RA118.006552. Epub 2019 Jan 7.
See all publications »
Publications resulting from the use of instrumentation should reference support from NIH NCRR grants 1S10 RR026501 and 1S10 RR027241, as well as NIH NIAID P30 AI078498 and the University of Rochester SMD.