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Mosmann Lab

Welcome to the Mosmann Lab

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The Th1 and Th2 subsets of CD4+ T cells induce different types of effector functions that are useful in combating different pathogens. Th1 cells producing Interferon gamma induce cytotoxic, inflammatory responses that are most effective against intracellular parasites, whereas Th2 cells producing IL4 and IL5 induce successful responses against helminth parasites. Although naïve, uncommitted CD4 T cells can differentiate into Th1 or Th2 phenotypes within a few days of initial stimulation, they can also remain uncommitted. These primed, precursor cells (Thpp) produce IL-2 and proliferate rapidly, allowing expansion of antigen-specific cells during an immune response before commitment to a particular effector phenotype. Cells producing only IL-2 also persist for several weeks after immunization, suggesting that these cells may also provide an expanded pool of uncommitted T cells for subsequent immune responses. In common with Treg cells, Thpp express CD73, which can help to generate adenosine that contributes to immune suppression.

In vivo T cell functions are difficult to evaluate, because cytokine secretion phenotypes in addition to Th1, Th2 and Th0 may occur in vivo; and differentiation and selective growth of T cell subsets in vitro can rapidly obscure patterns that occur in vivo. We are using multicolor flow cytometry and Spot assays to detect the simultaneous expression of six or more cytokines by individual human or mouse T cells, to resolve the contributions of partial differentiation, stochastic variation and environmental effects to the spectrum of cytokines produced by individual cells. Short-term cloning experiments are being used to examine the relationship between T cell phenotypes in vivo, versus in long-term tissue culture. These methods are being applied to define the precise T cell phenotypes induced by vaccination and infection by different influenza strains in infants, adults and the elderly.

A Genechip analysis of T cell gene expression revealed that Amphiregulin, an EGF family member, is selectively expressed in activated mouse Th2 cells, and contributes to Th2-mediated elimination of a helminth parasite. This raises the possibility that Th2 cells could contribute to asthma both by enhancing the allergic response and by increasing tissue remodeling. Initial human experiments suggest that regulation of amhipregulin expression is more complex in humans, but still linked to a Type 2 response. A clinical study has been initiated to compare amphiregulin expression in asthmatic patients and controls.

Tim R. Mosmann, Ph.D.

Tim R. Mosmann, Ph.D.

Principal Investigator

Publications

    1. Cossarizza A
    2. Chang HD
    3. Radbruch A
    4. Andrä I
    5. Annunziato F
    6. Bacher P
    7. Barnaba V
    8. Battistini L
    9. Bauer WM
    10. Baumgart S
    11. Becher B
    12. Beisker W
    13. Berek C
    14. Blanco A
    15. Borsellino G
    16. Boulais PE
    17. Brinkman RR
    18. Büscher M
    19. Busch DH
    20. Bushnell TP
    21. Cao X
    22. Cavani A
    23. Chattopadhyay PK
    24. Cheng Q
    25. Chow S
    26. Clerici M
    27. Cooke A
    28. Cosma A
    29. Cosmi L
    30. Cumano A
    31. Dang VD
    32. Davies D
    33. De Biasi S
    34. Del Zotto G
    35. Della Bella S
    36. Dellabona P
    37. Deniz G
    38. Dessing M
    39. Diefenbach A
    40. Di Santo J
    41. Dieli F
    42. Dolf A
    43. Donnenberg VS
    44. Dörner T
    45. Ehrhardt GRA
    46. Endl E
    47. Engel P
    48. Engelhardt B
    49. Esser C
    50. Everts B
    51. Falk CS
    52. Fehniger TA
    53. Filby A
    54. Fillatreau S
    55. Follo M
    56. Förster I
    57. Foster J
    58. Foulds GA
    59. Frenette PS
    60. Galbraith D
    61. Garbi N
    62. García-Godoy MD
    63. Ghoreschi K
    64. Gibellini L
    65. Goettlinger C
    66. Goodyear CS
    67. Gori A
    68. Grogan J
    69. Gross M
    70. Grützkau A
    71. Grummitt D
    72. Hahn J
    73. Hammer Q
    74. Hauser AE
    75. Haviland DL
    76. Hedley D
    77. Herrera G
    78. Herrmann M
    79. Hiepe F
    80. Holland T
    81. Hombrink P
    82. Houston JP
    83. Hoyer BF
    84. Huang B
    85. Hunter CA
    86. Iannone A
    87. Jäck HM
    88. Jávega B
    89. Jonjic S
    90. Juelke K
    91. Jung S
    92. Kaiser T
    93. Kalina T
    94. Keller B
    95. Khan S
    96. Kienhöfer D
    97. Kroneis T
    98. Kunkel D
    99. Kurts C
    100. Kvistborg P
    101. Lannigan J
    102. Lantz O
    103. Larbi A
    104. LeibundGut-Landmann S
    105. Leipold MD
    106. Levings MK
    107. Litwin V
    108. Liu Y
    109. Lohoff M
    110. Lombardi G
    111. Lopez L
    112. Lovett-Racke A
    113. Lubberts E
    114. Ludewig B
    115. Lugli E
    116. Maecker HT
    117. Martrus G
    118. Matarese G
    119. Maueröder C
    120. McGrath M
    121. McInnes I
    122. Mei HE
    123. Melchers F
    124. Melzer S
    125. Mielenz D
    126. Mills K
    127. Mjösberg J
    128. Moore J
    129. Moran B
    130. Moretta A
    131. Moretta L
    132. Mosmann TR
    133. Müller S
    134. Müller W
    135. Münz C
    136. Multhoff G
    137. Munoz LE
    138. Murphy KM
    139. Nakayama T
    140. Nasi M
    141. Neudörfl C
    142. Nolan J
    143. Nourshargh S
    144. O'Connor JE
    145. Ouyang W
    146. Oxenius A
    147. Palankar R
    148. Panse I
    149. Peterson P
    150. Peth C
    151. Petriz J
    152. Philips D
    153. Pickl W
    154. Piconese S
    155. Pinti M
    156. Pockley AG
    157. Podolska MJ
    158. Pucillo C
    159. Quataert SA
    160. Radstake TRDJ
    161. Rajwa B
    162. Rebhahn JA
    163. Recktenwald D
    164. Remmerswaal EBM
    165. Rezvani K
    166. Rico LG
    167. Robinson JP
    168. Romagnani C
    169. Rubartelli A
    170. Ruland J
    171. Sakaguchi S
    172. Sala-de-Oyanguren F
    173. Samstag Y
    174. Sanderson S
    175. Sawitzki B
    176. Scheffold A
    177. Schiemann M
    178. Schildberg F
    179. Schimisky E
    180. Schmid SA
    181. Schmitt S
    182. Schober K
    183. Schüler T
    184. Schulz AR
    185. Schumacher T
    186. Scotta C
    187. Shankey TV
    188. Shemer A
    189. Simon AK
    190. Spidlen J
    191. Stall AM
    192. Stark R
    193. Stehle C
    194. Stein M
    195. Steinmetz T
    196. Stockinger H
    197. Takahama Y
    198. Tarnok A
    199. Tian Z
    200. Toldi G
    201. Tornack J
    202. Traggiai E
    203. Trotter J
    204. Ulrich H
    205. van der Braber M
    206. van Lier RAW
    207. Veldhoen M
    208. Vento-Asturias S
    209. Vieira P
    210. Voehringer D
    211. Volk HD
    212. von Volkmann K
    213. Waisman A
    214. Walker R
    215. Ward MD
    216. Warnatz K
    217. Warth S
    218. Watson JV
    219. Watzl C
    220. Wegener L
    221. Wiedemann A
    222. Wienands J
    223. Willimsky G
    224. Wing J
    225. Wurst P
    226. Yu L
    227. Yue A
    228. Zhang Q
    229. Zhao Y
    230. Ziegler S
    231. Zimmermann J
    Guidelines for the use of flow cytometry and cell sorting in immunological studies.; European journal of immunology; Vol 47(10). 2017 Oct.
    1. Basha S
    2. Kaur R
    3. Mosmann TR
    4. Pichichero ME
    Reduced T-Helper 17 Responses to Streptococcus pneumoniae in Infection-Prone Children Can Be Rescued by Addition of Innate Cytokines.; The Journal of infectious diseases; Vol 215(8). 2017 Apr 15.
    1. Pedersen NW
    2. Chandran PA
    3. Qian Y
    4. Rebhahn J
    5. Petersen NV
    6. Hoff MD
    7. White S
    8. Lee AJ
    9. Stanton R
    10. Halgreen C
    11. Jakobsen K
    12. Mosmann T
    13. Gouttefangeas C
    14. Chan C
    15. Scheuermann RH
    16. Hadrup SR
    Automated Analysis of Flow Cytometry Data to Reduce Inter-Lab Variation in the Detection of Major Histocompatibility Complex Multimer-Binding T Cells.; Frontiers in immunology; Vol 8. 2017.
    1. Fiorentino DF
    2. Zlotnik A
    3. Vieira P
    4. Mosmann TR
    5. Howard M
    6. Moore KW
    7. O'Garra A
    Pillars Article: IL-10 Acts on the Antigen-presenting Cell to Inhibit Cytokine Production by Thl Cells. J. Immunol. 1991. 146: 3444-3451.; Journal of immunology (Baltimore, Md. : 1950); Vol 197(5). 2016 Sep 01.

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  Mosmann Lab
KMRB 3-9872
601 Elmwood Ave,
Rochester, NY 14642

 (585) 275-9445

  (585) 273-1434