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Histology Forms and Protocols

Internal Forms

Internal Work Order Form – Histology

Internal Justification Form – Histology

General Protocols

Histology Guidelines Presentation

Fixation of Skeletal Tissues – Paraffin

Fixation of Skeletal Tissues – Frozen

Preparation of Frozen Specimens

Slide Preparation

Histology Staining Protocols

Hematoxylin and Eosin Stain

Alcian Blue/Orange G Stains

Safranin O/Fast Green

Toluidene Blue/Fast Green

TRAP stain for Paraffin Sections

LacZ stain – Frozen Tissue Sections 1

LacZ stain – Frozen Tissue Sections 2

Von Kossa Staining

IHC Protocols

Aggrecan - IHC

Aggrecan (NITEGE) - IHC

Aggrecan (DIPEN) - IHC

Anti-PTH/PTHrP Receptor - IHC

Beta Galactosidase (LacZ) - IHC

Beta Catenin - IHC

Beta Catenin (Active) - IHC

BMP-2 - IHC

Cadherin11 - IHC

Collagen Type 1a1 - IHC

Collagen Type 2a1 – IHC

Collagen Type 3a1 - IHC

Collagen Type 10a1 – IHC

GFP - IHC

Lubricin (Prg4) - IHC

Matrix Metalloproteinase 13 – IHC

P27 - IHC

PECAM (CD31) - IHC

PPAR Gamma - IHC

Perilipin - IHC

Phosphorylated SMAD 1/5/8 – IHC

RANK - IHC

Runx2 - IHC

Sox-9 - IHC

Tak1 - IHC

TNF alpha - IHC

TRAP - IHC

VEGF - IHC

VEGF Receptor 2 - IHC

ISH Protocols

In Situ Hybridization – Radiolabeled 35S

In Situ Hybridization - Digoxigenin