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Histology Forms and Protocols

Internal Forms

Internal Work Order Form – Histology

General Protocols

Histology Guidelines Presentation

Tissue Submission for Paraffin Processing

Preparation of Frozen Tissue Specimens

Embedding Frozen Tissues

Slide Preparation

HBMI Core Decalcification Solutions

Histology Staining Protocols

Hematoxylin and Eosin Stain

Alcian Blue/Orange G Stains

Safranin O/Fast Green

Toluidene Blue/Fast Green

TRAP stain for Paraffin Sections

Beta Galactosidase Staining - Frozen Tissue Sections

Von Kossa Staining

IHC/IF Protocols

Aggrecan - IHC

Aggrecan (NITEGE) - IHC

Aggrecan (DIPEN) - IHC

Anti-PTH/PTHrP Receptor - IHC

Beta Galactosidase (LacZ) - IHC

Beta Catenin - IHC

Beta Catenin (Active) - IHC

BMP-2 - IHC

Cadherin11 - IHC

Collagen Type 1a1 - IHC

Collagen Type 2a1 – IHC

Collagen Type 3a1 - IHC

Collagen Type 10a1 – IHC

F4/80 - IF

GFP - IHC

Lubricin (Prg4) - IHC

Matrix Metalloproteinase 13 – IHC

P27 - IHC

PECAM (CD31) - IHC

PPAR Gamma - IHC

Perilipin - IHC

Phosphorylated SMAD 1/5/8 – IHC

RANK - IHC

Runx2 - IHC

Sox-9 - IHC

Tak1 - IHC

TNF alpha - IHC

TRAP - IHC

VEGF - IHC

VEGF Receptor 2 - IHC

ISH Protocols

In Situ Hybridization – Radiolabeled 35S

In Situ Hybridization - Digoxigenin