Does this test have other names?
Mono test, monospot test, Epstein-Barr test
What is this test?
This test looks for signs in your blood that you have the Epstein-Barr virus.
The Epstein-Barr virus (EBV) is a common virus that's part of the herpes virus family.
It causes infectious mononucleosis, or mono. Mono is passed from person to person
through saliva. Symptoms usually appear within four to six weeks after exposure and
ease in one to two months.
If you have mono, you may have a high level of a type of white blood cell called a
lymphocyte in your blood. Your immune system also will make heterophile antibodies
to fight off the EBV. These antibodies will also appear in your blood if you have
Why do I need this test?
You may have this blood test if your healthcare provider suspects that you have mononucleosis.
Signs and symptoms include:
Fatigue or exhaustion
What other tests might I have along with this test?
Your healthcare provider may also order a test for EBV-specific antibodies to confirm
the results of your blood test and get a definitive mono diagnosis.
Your healthcare provider may also order a complete blood count (CBC) and chest x-ray.
What do my test results mean?
Many things may affect your lab test results. These include the method each lab uses
to do the test. Even if your test results are different from the normal value, you
may not have a problem. To learn what the results mean for you, talk with your healthcare
Results are given in micrograms per liter (mcg/L). Normal ranges for lymphocytes are
1,000 to 4,800 mcg/L. The normal value for heterophile antibodies is zero.
If you have high levels of lymphocytes and heterophile antibodies are found, it means
that you may have mononucleosis.
How is this test done?
The test requires a blood sample, which is drawn through a needle from a vein in your
Does this test pose any risks?
Taking a blood sample with a needle carries risks that include bleeding, infection,
bruising, or feeling dizzy. When the needle pricks your arm, you may feel a slight
stinging sensation or pain. Afterward, the site may be slightly sore.
What might affect my test results?
HIV, lupus, lymphoma, rubella, hepatitis, and other viral infections may cause a false-positive
How do I get ready for this test?
You don't need to prepare for this test. But be sure your healthcare provider knows
about all medicines, herbs, vitamins, and supplements you are taking. This includes
medicines that don't need a prescription and any illicit drugs you may use.