ABO Immune Complexes Cause Endothelial Cell Damage

We are using electric cell-substrate impedance sensing (ECIS) and confocal microscopy, as well as other assays, to observe and quantify endothelial cell damage after exposure to non-identical ABO type plasma in vitro. This involves the generation of ABO immune complexes by mixing concentrated O and A plasmas together, the effects of which mimic ABO non-identical transfusion in vivo.

Healthy endothelial cells (below, left) versus 15 minutes post-exposure to immune complexes (right). There are noticeable changes to the cell morphology after incubation with immune complexes.

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