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Student Seminars

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NSC 503 Seminar

Tracey Preko, Pavel Rjabtsenkov, & Adam Roszczyk - PhD Candidates

Titles: TBD

Faculty Evaluators: Juliette McGreger & Chris Proschel

Student Moderator: Stacey Pedraza

 Dec 09, 2024 @ 4:00 p.m.

 Medical Center | K-207 (2-6408)

NSC 503 Seminar

Skylar DeWitt, Wen Li, & Gueladouan Jean Setenet - PhD Candidates

Titles: TBD

Faculty Evaluators: Liz Romanski & Jennetta Hammond

Student Moderator: Staci Rocco

 Dec 02, 2024 @ 4:00 p.m.

 Medical Center | K-207 (2-6408)

NSC 503 Seminar

Emma Bryson, Yunshan Cai, & Alex Solorzano - PhD Candidates

Titles: TBD

Faculty Evaluators: Chris Holt & Harris Gelbard

Student Moderator: Nicole Popp

 Nov 25, 2024 @ 4:00 p.m.

 Medical Center | K-207 (2-6408)

NSC 503 Seminar

Joanne Chiu & Alesandra Martin - PhD Candidates

Titles: TBD

Faculty Evaluators: Angela Hewitt & Krishnan Padmanabhan

Student Moderator: Leah Sheppard

 Nov 18, 2024 @ 4:00 p.m.

 Medical Center | K-207 (2-6408)

NSC 503 Seminar

Lia Calcinez Rodruiguez & Thomas Delgado (MSTP) - PhD Candidates

Titles: TBD

Faculty Evaluators: Julian Meeks & Adam Synder

Student Moderator: Niki Lam

 Nov 11, 2024 @ 4:00 p.m.

 Medical Center | K-207 (2-6408)

NSC 503 Seminar

Mark Osabutey & Leslie Gonzalez - PhD Candidates

Titles: TBD

Faculty Evaluators: Ed Lalor & David Kornack

Student Moderator: Aaron Huynh

 Nov 04, 2024 @ 4:00 p.m.

 Medical Center | K-207 (2-6408)

NSC 503 Seminar

Binjyu Sun & Julia Granato - PhD Candidates

Titles: TBD

Faculty Evaluators: Kuan Hong Wang & Laurel Carney

Student Moderator: AJ (Aishwarya) Jayan

 Oct 28, 2024 @ 4:00 p.m.

 Medical Center | K-207 (2-6408)

NSC 503 Seminar

Michael (Mike) Giannetto & John Gonzalez Amoretti - PhD Candidates

Titles: TBD

Faculty Evaluators: Julie Fudge & Eric Anson

Student Moderator: Erica Squire

 Oct 21, 2024 @ 4:00 p.m.

 Medical Center | K-207 (2-6408)

Unraveling the Effects of Inflammation on Visual Pathways: Insights into Schizophrenia-Related Dysfunction - Thesis Proposal

Tanique McDonald - PhD Candidate, Neuroscience Graduate Program

Schizophrenia is a chronic neuropsychiatric disorder that causes severe cognitive and functional impairments and is associated with premature mortality. In conjunction with the debilitating hallmark symptoms (i.e., delusions, hallucinations, and disorganized speech), schizophrenia is also associated with a range of visual deficits that alter perception and contribute to delusion formation. Notable visual system changes include retinal atrophy, reduced retinal signaling, altered contrast sensitivity, and reduced surround suppression. Like the overall disorder, the cause of these visual deficits remains unknown. However, an emerging body of literature suggests that elevated levels of the inflammatory cytokine, TNFa, triggers apoptosis of retinal ganglion cells, which is associated with degenerative effects along the feedforward visual pathway. These neuroprogressive effects are consistent with the aforementioned visual deficits commonly observed in patients with schizophrenia. Additionally, prior research has revealed elevated ocular and systemic levels of TNFa in patients with schizophrenia. Despite this, the link between ocular inflammation and visual deficits in schizophrenia remains speculative. The central hypothesis of this proposal is that there are significant positive relationships between higher ocular TNFa concentrations and the visual system changes commonly observed in patients with schizophrenia. Experiments proposed in Aim 1 will quantify ocular TNFa levels in patients and correlate these findings with measurements of retinal structure, visual system processing, and visual perception. Experiments proposed in Aim 2 will use a newly developed Mustela putorius furo (ferret) model to specify mechanistic electrophysiological changes to early components of the visual system (retina, visual thalamus, primary visual cortex) following exacerbation of ocular inflammation via TNFa. The proposed translational study will clarify the cellular and electrophysiologic bases for specific visual system deficits and validate the first animal model of visual anatomical and perceptual impairments in schizophrenia. This work also has potential implications for the development of biomarkers for early detection and monitoring of neuroprogression, and for animal modeling and other research strategies addressing visual system changes associated with neurodegenerative, and psychosis-related disorders.

 Oct 11, 2024 @ 2:00 p.m.

 Medical Center | K207 (2-6408)

Host: Advisors: Farran Briggs, PhD and Steven Silverstein, PhD

Combinatorial Effects of Stress and Perfluorooctanoic Acid on Pregnancy - Thesis Proposal

Erin Murray - PhD Candidate, Neuroscience Graduate Program

Depression, one of the leading causes of disability worldwide, manifests in various subtypes, including postpartum depression (PPD), which presents after the critical window of pregnancy. Two common, co-occurring environmental factors, exposure to endocrine disrupting chemicals (EDCs) and psychological stress, both disrupt hypothalamic-pituitary-adrenal (HPA) function. Independently, these environmental factors are both associated with increased risk for PPD. Stress can alter neurotransmitter balance and neuroplasticity, trigger neuroinflammation, and produce depressive-like behaviors, such as reduced motivation. Of the many EDCs, perfluorooctanoic acid (PFOA) is found ubiquitously in nearly all pregnant women and infants. Although PFOA and stress both act upon the HPA axis, it remains to be determined whether co-exposure to these factors results in enhanced hormone production or enhanced disruption of the metabolic, immunological, and neurological changes that occur during pregnancy that are related to depressive-like behaviors. Preliminary data from our laboratory has shown that mouse dams exposed to stress and PFOA (100 ng/kg/day - PFOA+S) exhibit unique increases in serum corticosterone and altered metabolic profiles within the frontal cortex (FC), a region found dysregulated in patients with depression that provides critical modulatory feedback to reward-related brain regions. Additionally, our data indicates stress induced increases in activity and self-directed behaviors (grooming and increased localized spontaneous movement). However, effects on motivation, a more translationally relevant behavioral domain for depression, have yet to be evaluated. It is unclear if motivation and stress-induced activity-related behaviors are modulated by the same underlying mechanisms during pregnancy and in the postpartum period. Furthermore, HPA axis activation drives the metabolic support critical for energic homeostasis throughout pregnancy and modulates production of cytokines necessary for immune function and suppression critical to fetal growth and development. Tryptophan metabolism is known to be regulated by both immune and HPA axis, and tryptophan concentrations decrease across gestation in both serum and in brain. Therefore, it is important to understand how the bidirectional regulation between the immune and metabolic systems may be dysregulated in dams exposed to PFOA+S and how this potential dysregulation may affect behavior. To address these questions, we will use a novel resource deprivation paradigm modeling psychosocial stress to examine the combinatorial effects of both gestational stress and low dose PFOA on maternal brain and behavior in C57Bl6/J mice. We aim to 1) test the hypothesis that PFOA plus stress reduces motivation in pregnant dams by developing a behavioral assay of motivation for pup access, 2) identify molecular targets of stress-induced increases in stereotypic behavior and increased self-grooming and motivation, and 3) explore how endocrine activation by PFOA and stress modulates the relationship between tryptophan metabolism and immune markers. In addition to improving upon current rodent models of psychosocial stress in females, which is critical for strengthening the toolset used to study PPD, this project is essential for advancing a cumulative risk framework for “safe” chemical exposure levels in vulnerable populations.

 Sep 27, 2024 @ 9:00 a.m.

 Medical Center | K307 (3-6408)

Host: Advisor: Marissa Sobolewski, PhD

Multi-Modal Markers for Deep Brain Stimulation Modulation of Cognitive and Motor Functions in Parkinson’s Disease - Thesis Proposal

Jeehyun Kim - PhD Candidate, Neuroscience Graduate Program

Parkinson’s disease (PD) is a progressive neurodegenerative disease that significantly impairs both motor and cognitive functions, including working memory, which are often under-recognized and inadequately addressed by treatments, such as deep brain stimulation (DBS). Understanding the effects DBS, such as in the subthalamic nucleus (STN-DBS), on these functions is crucial for optimizing therapeutic interventions and enhancing patient care. While STN-DBS offers motor symptom relief, it has mixed cognitive outcomes, and its differential impact under varying cognitive loads and stimulation states is not well understood. There is a critical need to better understand the neural mechanisms underlying such STN-DBS effects and to develop objective, multi-modal measures to assess these effects more comprehensively. The proposed project aims to develop and utilize multi-modal measures to elucidate the neural mechanisms underlying cognitive and motor functions in PD and their responses to STN-DBS, thereby enhancing our understanding of the mechanisms and clinical effectiveness of STN-DBS. Aim 1 characterizes the multi-modal measures of cognitive (working memory) and motor (upper extremity motor control) functions in patients with PD compared to neurotypical controls using a Mobile Brain-Body Imaging (MoBI) system. Aim 2 investigates the effects of different STN-DBS states (bilateral off, bilateral on, left-on only, right-on only) on these measures in the same individuals. The MoBI system simultaneously records high-density electroencephalogram (EEG), 3D eye/motion-tracking, and cognitive performance during a visuospatial working memory task with varying memory loads and movement incorporated recall responses. Key multi-modal measures include event-related potential (ERP) components, such as Contralateral Delay Activity (CDA), along with cognitive and motor performance measures. The hypothesis is that STN-DBS will differentially impact cognitive and motor functions based on memory loads and stimulation states. Changes in ERPs will represent the underlying neural mechanisms explaining these behavioral outcomes. It is expected that comparing bilateral and unilateral STN-DBS effects will also reveal how different stimulation states impact cognitive and motor functions, considering task-specific, handedness specific, and disease-specific laterality. This project aims to establish comprehensive multi-modal markers for assessing cognitive and motor functions and their modulation by STN-DBS, ultimately contributing to more effective and personalized care for each patient with PD.

 Sep 25, 2024 @ 8:00 a.m.

 Medical Center | 1-9523/35 Northeastern Rm

Host: Advisors: Ed Freedman, PhD & John Foxe, PhD

NSC 503 Seminar

Dennisha King & Abigail Sawicki-Mayer - PhD Candidates

Titles:  “Investigating the role of PPP2R5D and its human de novo mutations in liprin-alpha 1 regulation" - Mayer

Faculty Evaluators: M. Kerry O'Banion & Ross Maddox

Student Moderator: Alesha Anchan

 Sep 23, 2024 @ 4:00 p.m.

 Medical Center | K-207 (2-6408)

NSC 503 Seminar

Yanya Ding & Evan Newbold - PhD Candidates

Titles: Sex-specific and age-related progressions of auditory neurophysiological deficits in the Cln3 -/- mouse model of Batten Disease - Ding
Contractile modulation of cervical lymphatic vessels improves cerebral clearance - Newbold

Faculty Evaluators: Pat White & Archan Ganguly

Student Moderator: Sid Chittaranjan

 Sep 16, 2024 @ 4:00 p.m.

 Medical Center | K-207 (2-6408)

NSC 503 Seminar

Victoria Popov & Paige Nicklas - PhD Candidates

Titles:  Making Strides: Cognitive-Motor Interactions in Autism Spectrum Disorder - Nicklas
An acute exercise intervention to ameliorate behavioral and neurophysiological indices of inhibitory control deficits in schizophrenia: A Mobile Brain/Body Imaging (MoBI) study - Popov

Faculty Evaluators: Manuel-Gomes-Ramirez & David MacLean

Student Moderator: Erin Murray

 Sep 09, 2024 @ 4:00 p.m.

 Medical Center | K-207 (2-6408)

Research symposium in honor of Marc Schieber, MD, PHD

Marc Schieber, MD, PHD - Professor, University of Rochester

The Departments of Neuroscience and Neurology, and the Del Monte Institute for Neuroscience welcome you to a research symposium in honor of Marc Schieber, MD, PHD

Friday, September 6, 2024, 1:00 – 5:15 pm
1-7619 Adolph Auditorium

Reception in the Forbes Mezzanine to follow

View Announcement  View Event Schedule

Speakers include:

  • Catherine Lang, PhD, Professor of Physical Therapy, Neurology and Occupational Therapy, Washington University 
    “Wearable sensors are changing the way we think about movement and neurorehabilitation”
  • Kevin Mazurek, PhD, Assistant Professor of Neurology and Biomedical Engineering, Mayo Clinic, Rochester, MN 
    “Movement with an Element of Style: Teaching Precision, One Finger at a Time”
  • Adam Rouse, MD, PhD, Dept. Neurosurgery, University of Kansas Medical Center 
    “Exploring motor cortex, why so many neurons?”
  • Marco Santello, PhD, Fulton Professor of Neural Engineering, Arizona State University 
    “Insights on neural control of the hand through multidisciplinary research approaches”

 Sep 06, 2024 @ 1:00 p.m.

 Medical Center | 1-7619 Adolph Aud

Host: The Departments of Neuroscience and Neurology, and the Del Monte Institute for Neuroscience

NSC 503 Seminar: Introduction

Faculty Evaluators:  Gabriella Sterne & Brian Keane

 Aug 26, 2024 @ 4:00 p.m.

 Medical Center | L-307 (3-6408)

The role of reactive microglia in glaucoma - Thesis Proposal

Sean Lydon - PhD Candidate, Neuroscience PhD degree program

 Aug 23, 2024 @ 1:00 p.m.

 Medical Center | 1-9525 Northeastern Rm

Host: Advisor: Richard Libby, PhD

Tools to Explore the Neural Control of Vision-Guided Reaching Behavior for Dynamic Targets in the Common Marmoset - Thesis Defense

Luke Shaw - PhD Candidate, Neuroscience PhD degree program

Primates manipulate features of their environment using their forelimbs and dexterous hands, which are commonly guided by visual perception. It is known that in manual interception of moving targets, humans can make rapid adjustments to rapidly compensate for changes in motion. To date, we lack a complete understanding of the neural control of dynamic reaching. This research first characterizes reaching in a new world primate model, the common marmoset (Callithrix jacchus), and further demonstrates methods to inhibit specific components of the reaching neural control circuit during reaching.

To study dynamic reaching in marmosets, we use an ecologically relevant approach with reaching for live moving crickets. Using this paradigm, we estimate visuomotor delay and the time constant to which marmosets predict future target position, confirming that marmosets share several aspects of predictive reaching found in human interception. We further extend this paradigm to include mechanically controlled targets capable of mimicking the motion of a live cricket. Using this apparatus, we validate findings from live cricket reaching experiments with higher trial yields and novel target motion behavior.

In primates, control of vision-guided reaching involves tight coordination of motor planning centers in premotor cortex and vision processing centers in parietal cortex. It is thought that premotor cortex relays motor information back to parietal cortex enabling prediction and also short visuomotor delays. Here, we test and optimize optogenetic tools for the marmoset to manipulate feedback projections from premotor cortex to parietal cortex and their role in prediction. To accomplish this, we utilize an intersectional approach to express light activated ion channels, or opsins, in feedback projections and validate their efficacy in histology and with electrophysiological recordings using optical stimulation. We also test opsins expressed specifically in inhibitory interneurons to enable rapid suppression of premotor or parietal areas. These optogenetic manipulations when coordinated with reaching behavior will enable us to distinguish the contributions of feedback to predictive reaching.

Defense Announcement

 Aug 22, 2024 @ 1:30 p.m.

 Medical Center | 1-9525 Northeastern Rm.

Host: Advisors: Kuan Hong Wang, PhD and Jude Mitchell, PhD

Investigating the role of High mobility group box 1 (HMGB1) in postoperative delirium - Thesis Proposal

Mariah Marrero - PhD Candidate, Neuroscience Graduate Program

Postoperative delirium (POD) is the most prevalent surgical complication in geriatric patients – with a global incidence rate of 20% and a three-fold likelihood among individuals over the age of 65. POD is strongly associated with neurocognitive decline (NCD), leading to faster rates of cognitive decline with memory deficits. Despite its socioeconomic burden and high incidence rate, the lack of preventative or therapeutic strategies is a pressing issue, largely due to research gaps in molecular signatures of pathology. Clinical evidence shows that increased CSF and High mobility group box 1 (HMGB1) protein plasma are strong predictors for POD and neurocognitive decline. HMGB1 is a DNA chaperone-binding protein localized to the nucleus, and when extracellularly released, binds to toll-like receptor-2/4 (TLR2/TLR4) and receptor for advanced glycation end products (RAGE), becoming immunologically active. Exogenous HMGB1 in the bloodstream has been shown to breach the blood-brain barrier (BBB) and enter the brain, increasing protein levels in areas such as the hippocampus. Previous literature shows that increased exogenous HMGB1 in the hippocampus can bind to RAGE receptors on microglia and increase secretion of complement protein C1q, leading to a feedforward loop of activation and synapse-elimination. This relationship has not been explored with POD. Therefore, I propose to investigate the role of HMGB1 signaling in the BBB and neuroinflammation in the context of sterile surgery, with the potential to advance our understanding significantly and potentially lead to developing new preventative or therapeutic strategies for POD. I hypothesize that exogenous HMGB1 from innate immune cells will inflame and disrupt the BBB, causing soluble HMGB1 to enter the hippocampus and activate microglia. Specifically, HMGB1-induced microglia activation will increase complement secretion of C1q, thereby dysregulating microglia-dependent synaptic elimination. I will use a Transwell system to model the blood murine brain microvascular endothelial cells (BMECs) and pericytes (PCs) to address these unknown mechanisms to simulate vascular changes in response to HMGB1. I will also use a mouse model of orthopedic surgery (rod-fixed tibial fractures) analogous to sterile surgical trauma, where we will collect blood and brain samples over 24 hours using both young (3-month-old) and old (20-month-old) mice to address age-related changes. Preliminary data from our lab found significant increases in plasma HMGB1 1 hour and an increase in HMGB1 expression 24 hours after surgery in young mice versus sham. Using these two models; I will investigate HMGB1 signaling in immune cells, BBB, and microglial activation/C1q activation.

Announcement Flyer

 Aug 22, 2024 @ 12:00 p.m.

 Medical Center | 2-6424 (SMD Large Aud.)

Host: Advisor: Harris Gelbard, PhD

The functional impact of cortical and retinal damage on the parallel processing streams in the early visual pathways - Thesis Defense

Jingi Yang - PhD Candidate, Neuroscience Graduate Program

The magnocellular and parvocellular pathways are the major parallel information processing streams of the early visual system. Signals relayed by these pathways from the retina to the dorsal lateral geniculate nucleus (dLGN), and then to primary visual cortex (V1) are critical for visual image processing and representation. When any of these pathways are disrupted, loss of vision occurs. The structure and function of each pathway have been well characterized. However, little is known about neurophysiological changes in these pathways following injury, such as in V1 stroke or retinal disease.

Previous work in non-human primate models of glaucoma suggests that there is a greater loss of cytochrome oxidase reactivity in parvocellular compared to magnocellular layers of the dLGN (Crawford et al., 2001, Sasaoka et al., 2008), suggesting stream-specific effects of retinal ganglion cell (RGC) degeneration. Furthermore, previous work in non-human primate models of V1 damage suggests relative preservation of visually responsive magnocellular neurons in the dLGN after long-term V1 damage (Yu et al., 2018), and visual training-induced recovery in occipital cortical stroke patients is more effective with motion stimuli, i.e., stimuli that activate the magnocellular stream. My thesis aimed to understand whether functional plasticity in the early visual pathways following cortical or retinal damage is stream-specific.

We hypothesized that both RGC loss and V1 lesions caused by stroke could differentially affect the magnocellular or parvocellular pathways. In the first study of an animal (ferret) model of RGC loss, I examined the impact of the retinal excitotoxic lesions on the physiological response properties of dLGN neurons. Although the majority of contralesional transient and sustained dLGN neurons lost tuning to contrast, sustained neurons had longer response latencies, greater variability in their responses to visual stimuli, and greater changes in their tuning preferences compared to transient neurons. In the second study of visual training-induced recovery in human stroke patients, I showed that adaptive training with static, drifting, or flickering Gabor patches of progressively lower contrasts improved contrast sensitivity for both orientation and motion discrimination in cortically-blind (vision loss due to V1 stroke) participants; however, normal contrast sensitivity was not recovered in any participant. In summary, this work suggests that: (1) More RGCs in the X (parvocellular-like) stream than those in the Y (magnocellular-like) stream degenerate following kainic acid injections into the eye, (2) sustained (parvocellular-like) neurons in the ferret dLGN appear to be less functionally resistant to degeneration 7 days following retinal lesions compared to transient (magnocellular-like) dLGN neurons, (3) lesion to V1 induces a rapid and severe impairment of contrast sensitivity for orientation and motion direction discrimination in the affected hemifield, (4) adaptive training with stimuli containing higher temporal frequencies, optimal for magnocellular pathway, is not more effective than static stimuli. Together, these findings suggest that post-injury functional plasticity in the early visual pathways depends not only on the parallel streams but also on the location of the injury and the type of the injury.

 Jul 03, 2024 @ 1:00 p.m.

 Medical Center | K-207 (2-6408)

Host: Advisors: Farran Briggs, PhD & Krystel Huxlin, PhD

Neurogenetic Mechanisms Underlying Sexually Dimorphic Behavioral States in C. elegans - Thesis Defense

Gregory Reilly, MS - PhD Candidate, Neuroscience Graduate Program

Biological sex is a fundamental dimension of internal state that can have deep influences on behavior. Understanding the mechanisms behind these influences can provide insight into how shared neural circuits are tuned to produce sex-specific behavioral variation. Biological sex can influence both short-term behaviors and longer, more persistent forms of behavior known as behavioral states. In C. elegans, persistent motor behavior, called locomotor states, is well-studied in hermaphrodites. On a patch of food, hermaphrodites will switch between two states of foraging and feeding, called roaming and dwelling respectively. However, while some work has examined motor states in males, these remain poorly characterized. Previous work from our lab has demonstrated that male locomotion is sex-specific; the sexual state of muscle tissue and the nervous system is essential for sex differences in speed and body posture. Therefore, biological sex may also similarly influence locomotor states. We trained a supervised machine learning Random Forest model to detect three locomotor states: roaming, dwelling, and tail chase. In addition, we used a dimensionality reduction analysis, Linear Discrimination Analysis (LDA), to compare the overall characteristics of these states. Furthermore, to measure the transition probability between states, we used a Markov model. While both males and hermaphrodites share the locomotor states of roaming and dwelling, the characteristics of these differ by sex- the amount of time spent in each state, state durations, and transitions between states (temporal differences), as well as the linear speed, curvature, and other characteristics (feature differences), have sexual dimorphism. To understand how sex tunes these locomotor states, we manipulated the sex determination pathway to sex reverse the nervous system in both males and hermaphrodites. Interestingly, we found that pan-neuronally feminized males had similar locomotor state characteristics to hermaphrodites; both temporal and state feature sex differences were eliminated in the feminized males. Yet, masculinized hermaphrodites were indistinguishable from their wildtype counterparts indicating that either male-specific neurons or other tissue played a role in mediating these sex differences. To uncover the mechanisms that biological sex leverages to achieve this sex-specific variance in locomotor states, various neuromodulator knockout mutants known to affect locomotor states were tested. PDFR-1 emerged as a strong candidate as it removed differences in both temporal and features of locomotor states. Preliminary data suggests that PDFR-1 signaling may regulate the temporal sex differences through daf-7, a TGF-ß signal. daf-7 knockout mutants appear to maintain differences in state features but both spend similar amounts of time roaming and dwelling. Given that PDFR-1 signaling has been implicated as the mechanism that regulates sexual dimorphism in daf-7 expression in the ASJ neuron, these results remain promising. Together, our results provide a mechanistic framework for understanding how sex-specific neuronal tuning influences behavioral states.

 Jun 14, 2024 @ 10:00 a.m.

 Medical Center | SMD Lg. Aud. (2-6424)

Host: Advisor: Doug Portman, PhD

Mechanisms of Cerebellar Microglial Dynamics and Their Influence On Behavior - Thesis Defense

Mark Stoessel, MS - PhD Candidate, Neuroscience Graduate Program

Synaptic plasticity allows the central nervous system to incorporate new sensory experiences and information, and its disruption is associated with many neurological and psychiatric disorders. Much recent work has focused on the contribution of non-neuronal central nervous system cells, especially microglia, to synaptic plasticity. Though classically defined by their immune capacities, microglia are vital to many homeostatic processes, including synaptic plasticity of nascent and adult neuronal networks. Despite the emerging consensus that microglial dynamics are critical to brain function during physiological as well as pathological conditions, it is unclear whether these microglial roles and their underlying mechanisms are universal or differ between brain regions. There is a growing body evidence to suggest microglia exhibit a high degree of regional specialization. Cerebellar microglia in particular exhibit unique transcriptional and epigenetic profiles, and distinct functional properties, such as being morphologically less ramified, and less densely distributed than cortical microglia. As a consequence, cerebellar microglia survey less of the parenchyma than cortical microglia but compensate for this by undergoing frequent somatic translocations under homeostatic conditions, a phenomenon not observed in cortex. Despite such differences, cerebellar microglia maintain common microglial functions. Two pathways of interest to cortical microglial mediated synaptic plasticity are purinergic signaling through the P2Y12 receptor and noradrenergic signaling through the β2 adrenergic receptor (β2-AR), both of which have been shown to be critically involved in microglial roles in synaptic remodeling and rapid chemotaxis to sites of injury.

To address this question of regional heterogeneity in microglial signaling we investigated the roles of P2Y12 and β2-AR in cerebellar microglial with a comparison to the known roles of these signaling pathways in cerebral cortex. We desired to understand the contribution of these pathways to the many aspects of microglial function in the adult brain and therefore characterized cerebellar microglial morphology, surveillance, injury response dynamics, gene expression patterns, and contributions to cerebellar learning and plasticity, while manipulating either microglial purinergic or adrenergic signaling. On the whole, our findings suggest that signaling pathways that are present in both cortical and cerebellar microglia may play differential roles in microglial function depending on brain area.

 Jun 12, 2024 @ 1:00 p.m.

 Medical Center | K-207 (2-6408)

Host: Advisor: Ania Majewska, PhD

NSC 503 Seminar

Amelia Hines; Andrea Campbell - PhD Candidate

Faculty Evaluators:  Jesse Schallek and Gabriella Sterne

Student Moderator:  Anthony Bryan Crum (Bryan)

 May 20, 2024 @ 4:00 p.m.

 Medical Center | K-307

Neurorehabilitation in Apraxia - “Between Thinking and Doing: Disorders at the Cognitive-motor Interface in Chronic Stroke”

Laurel Buxbaum, PsyD - Research Professor, Department of Rehabilitation Medicine, Institute Scientist, Moss Rehabilitation Research Institute

 May 15, 2024 @ 12:00 p.m.

 Medical Center | K-307 Room 3.6408

https://urmc.zoom.us/j/91250439556?pwd=cnVnTTloY3BRcGJpQ0FTMm5HeDdtUT09

NSC 503 Seminar

Aiesha Anchan; Gavin Magill - PhD Candidate

Faculty Evaluators:  Amy Kiernan and Kenneth Henry

Student Moderator:  Alexis Feidler

 May 13, 2024 @ 4:00 p.m.

 Medical Center | K-307

Effects of Developmental Ethanol Exposure on Cerebellar Microglia and Purkinje Cells - Thesis Defense

MaKenna Cealie - PhD Candidate, Neuroscience Graduate Program

Fetal alcohol spectrum disorders (FASD), caused by prenatal alcohol exposure, are the most common cause of non-heritable, preventable mental disability and have no known cure. Physical, cognitive, and behavioral deficits have been reported in FASD, including impairments related to the cerebellum. To elucidate the mechanisms of FASD, we examined microglia, the immune cells of the central nervous system, as well as Purkinje cells, the sole output of the cerebellar cortex, which are both impacted by developmental ethanol exposure. Microglia are dynamic cells and shape neuronal circuit development and connectivity in the cerebellum. However, how cerebellar microglia dynamics and their interactions with neurons are affected by early life exposure to ethanol is unknown. We explored the impact of a third-trimester equivalent binge-level ethanol exposure on cerebellar microglia and microglia-Purkinje cell interactions in adolescent and young adult mice.

We subcutaneously injected Ai9+/-/C3xcr1G/+/L7cre mice with 5.0 g/kg/day of either ethanol or saline from postnatal (P) days 4-9. Mice were then aged to adolescence (P28) and cranial windows were implanted above the cerebellum to allow for two-photon in vivo imaging in both adolescence and young adulthood (P60). We found that in vivo cerebellar microglia dynamics, microglia morphology, and microglia-Purkinje cell interactions were largely unaffected by developmental ethanol exposure in both adolescence and young adulthood. We also examined if a “second-hit” laser ablation injury in young adulthood would uncover differences, but found no changes in cerebellar microglia injury response between ethanol- and saline-dosed animals. We collected the young adults’ brains for confocal imaging to examine a larger number of microglia and Purkinje cells. Microglia density, morphology, and interactions with Purkinje cells were largely unaltered by developmental ethanol exposure. However, Purkinje cell linear frequency was significantly decreased in ethanol-dosed mice.

Overall, we found that cerebellar microglia in adolescent and young adult mice were largely unaffected by developmental ethanol exposure, but Purkinje cells appeared to be more susceptible to its effects. Our work suggests that microglia may return to homeostasis later in life after an early life insult. This work is important to narrow down the mechanisms leading to FASD so future therapies can be developed.

 May 13, 2024 @ 11:00 a.m.

 Medical Center | Lower Adolph Aud. (1-7619)

Host: Advisor: Ania Majewska, PhD

Investigating the critical in vivo role of neuronal PP1β - Thesis Defense

Cody McKee - PhD Candidate, Neuroscience Graduate Program

Protein Phosphatase 1 (PP1) is a major Serine (Ser)/Threonine (Thr) phosphatase responsible for more than half of all Ser/Thr dephosphorylation events in eukaryotic cells. Three genes encode the three major isoforms of PP1 (α, β, and γ). While PP1α and PP1γ are considered major players in synaptic physiology, the neuronal function of PP1β is unknown. Recently, de novo mutations in PP1β have been linked to intellectual developmental disabilities in children, suggesting a critical role for PP1β in the central nervous system. While correlations between PP1 and various other neurodevelopmental/neurodegenerative diseases have been suggested, a causative role for PP1 in many of these contexts has yet to be established. The current study seeks to investigate the neuronal role of PP1β in vivo, and to uncover potential mechanisms by which PP1β influences neuronal function.

A Thy1-Cre mouse line was used to generate neuron specific PP1β conditional KO (PP1β cKO) mice. These mice exhibit a failure to thrive and typically die by 2-3 postnatal weeks. Hippocampal slice recordings demonstrated increased paired-pulse facilitation, suggesting impaired neurotransmitter release. In agreement with studies suggesting activity influences myelination within specific brain regions, we found significantly lower levels of myelin basic protein in the cortex of PP1β cKO mice. Furthermore, to assess the influence of PP1β on myelin function in a predominately activity-independent context, we measured compound action potentials (CAPs) along the optic nerve. Deficits in CAP recordings suggested impaired optic nerve myelination. However, analysis of the electron micrographs failed to detect a significant difference in myelinated axons. Using immunofluorescence, we then uncovered significantly fewer nodes of Ranvier in PP1β cKO mice that could potentially explain the CAP recordings. This deficit in nodes coincided with an increase in phosphorylation of PP1β-specific substrate, myosin light chain, which localizes to nodes of Ranvier. These data suggest a potential role for PP1β in nodal structure that could influence action potential propagation.

To then study the role of PP1β in adolescent mice, we generated a neuron specific inducible PP1β KO mouse line (iKO). These iKO mice exhibit progressive deterioration of hind limb functionality and premature demise at ~4 weeks post recombination. We then uncovered significant changes in various respiratory parameters suggesting a potential mechanism to explain the premature demise. However, while no morphological changes were observed within neuromuscular junctions in the diaphragm, it is possible that neurotransmitter release at these synapses is abrogated, and this will be investigated in the future.

These data support the hypothesis that PP1β alters action potential propagation in a way that disrupts downstream functionality. These results shed light on the role of PP1β and potential mechanisms that could be disrupted by PP1β in pathological states. Future studies will seek to uncover the molecular substrates underlying these effects and provide potential therapeutic targets for diseases in which PP1β functionality may be altered.

 May 10, 2024 @ 2:00 p.m.

 Medical Center | K-207 (2-6408)

Host: Advisor: Houhui (Hugh) Xia

NSC 503 Seminar

Aishwarya Jayan; Leah Sheppard - PhD Candidate

Faculty Evaluators:  Todd Jusko and Joog-Hoon Nam

Student Moderator:  Thomas Delgado

 May 06, 2024 @ 4:00 p.m.

 Medical Center | K-307

The Elizabeth Doty Lecture - "The Internal Focus of Attention"

Anna Christina Nobre, PhD - Wu Tsai Professor of Psychology, Director, Center for Neurocognition and Behavior, Wu Tsai Institute, Yale Univiversity

Dr. Nobre’s talk will discuss her recent studies exploring how we focus attention to contents in working memory. Her findings highlight the flexibility of the internal focus – dynamically and proactively prioritizing or shielding different contents in turn to guide future behavior – as well as its sensitivity to the temporal structure of anticipated events and tasks.

 

 May 02, 2024 @ 4:00 p.m.

 Medical Center | Lower Adolph Aud. (1-7619)

NSC 503 Seminar

Niki Lam; Tom Scudder - PhD Candidate

Titles:  Disentangling the contributions of enhancement and suppression to selective visual processing - Lam
Cav1.3 Ca+ Current Mediation by the PACAP peptide - Scudder

Faculty Evaluators:  Paul Kammermeir and Robert Freeman

Student Moderator:  Abigail Sawicki

 Apr 22, 2024 @ 4:00 p.m.

 Medical Center | K-307

Annual Neuroscience Retreat

Dr. Takao Hensch - Keynote Speaker

 Apr 19, 2024 @ 8:30 a.m.

 Memorial Art Gallery | 

NSC 503 Seminar

Bingyu Sun; Bryan Redmond - PhD Candidate

Titles:  ADDaPT: automated direction discrimination perimetric task for detecting blind field visual abilities - Redmond
The Mechanistic Effects of Cannabidiol in an Amyloidosis Mouse Model - Sun

Faculty Evaluators:  J. Chris Holt and Thomas O'Connor

Student Moderator:  Michael (Mike) Giannetto

 Apr 15, 2024 @ 4:00 p.m.

 Medical Center | K-307

NSC 503 Seminar

Erica Squire; Staci Rocco - PhD Candidate

Titles:  Gustatory processing in Drosophila melanogaster and the role of ‘buddy - Squire
Evaluating the Cellular and Molecular effects of Basmisanil: A Potential Rapid Antidepressant for MDD - Rocco

Faculty Evaluators:  Debroah Cory-Slechta and Samuel Norman-Haignere

Student Moderator:  Evan Newbold

 Apr 01, 2024 @ 4:00 p.m.

 Medical Center | K-307

NSC 503 Seminar

Amelia Day Hines; Sid Chittaranjan - PhD Candidate, Neuroscience Graduate Program

Titles: Astrocytes modulate synaptic plasticity via norepinephrine - Hines
The Role of Astrocytes in Acute Insulin Resistance - Chittaranjan

Faculty Evaluators:  Jean Bidlack and Brian Keane

Student Moderator:  Victoria Popov

 Mar 25, 2024 @ 4:00 p.m.

 Medical Center | K-307

Thesis Defense: "Interfacing with the Cortical Reach-to-Grasp Network using Low-Amplitude Intracortical Microstimulation"

Brandon Ruszala - PhD Candidate, Biomedical Engineering PhD Program

Abstract: Movement is the primary way people interact with the world. Injuries to the nervous system that disrupt a person’s ability to move (e.g., losing a limb or paralysis) can be devastating. However, the cortical regions that control movement often remain intact and functional offering an interesting potential treatment – communicating with those still-functional brain regions to control a machine and bypass the injuries. In other words, establishing a brain-machine interface (BMI). Focusing on upper-extremity BMIs, robotic arms can be successfully controlled by decoding neurons from motor cortex and improved by delivering sensory feedback to somatosensory cortex with intracortical microstimulation (ICMS). However, controlling BMIs with the speed, accuracy, and precision of natural movements made with native limbs remains a challenge. This dissertation identifies several features of the cortical motor system that could be leveraged to refine BMI control. First, we show that neurons in motor cortex encode instruction modality – a non-kinematic feature that may constitute noise for BMIs trained to decode movement kinematics from those neurons. Accounting for such non-kinematic variation in future decoding algorithms may allow for more accurate decoding of movement parameters from that neural activity. Second, we show low-amplitude ICMS can modulate neurons in distant cortical regions. Initially, we show ICMS delivered in primary somatosensory cortex modulates the activity of neurons across wide territories in primary motor cortex and ventral premotor cortex. Subsequently, we show distant modulation effects can be produced over even greater spatial scale across the entire cortical reach-to-grasp network. For BMIs that concurrently stimulate neurons in some cortical regions while decoding neurons in others, modulation produced by the stimulation in decoded neurons can hinder decoder performance. Incorporating information about distant modulation effects into decoding algorithms could mitigate that hindrance. Finally, we explored the cortical reach-to-grasp network for novel regions in which information can be delivered using ICMS. We found that the ventral premotor cortex and the anterior intraparietal area were effective regions for delivering information, whereas the dorsal premotor cortex and dorsal posterior parietal cortex were ineffective. Future BMIs might deliver more complex information to the brain via those former regions, expanding the bidirectional brain-machine interface.

 Mar 19, 2024 @ 10:00 a.m.

 Medical Center | K-207 (2-6408)

Zoom Link

Host: Advisor: Marc Schieber, MD, PhD

NSC 503 Seminar

Aaron Huynh; Stacey Pedraza - PhD Candidate

Extra presentation by Amber Rivera, MSW, Director of Lerner Life and Wellness

Titles:  “Approach to Evaluate Post-Stroke Motor Rehabilitation” - Huynh
“Macrocyclic Tetrapeptides to Treat Cocaine Use Disorder” - Pedraza

Faculty Evaluators:  Chris Proschel and Kerry O'Banion

Student Moderator:  Yanya Ding

 Mar 18, 2024 @ 4:00 p.m.

 Medical Center | K-307

NSC 503 Seminar

Anthony Bryan Crum (Bryan); John Gonzalez Amoretti - PhD Candidate

Faculty Evaluators:  Lars Ross and Ania Majewska

Student Moderator:  Erin Murray

 Mar 04, 2024 @ 4:00 p.m.

 Medical Center | K-307

NSC 503 Seminar

Dennisha King; Paige Nicklas - PhD Candidate

Faculty Evaluators:  Harris Gelbard and Marc Schieber

Student Moderator:  Julia Granato

 Feb 26, 2024 @ 4:00 p.m.

 Medical Center | K-307

NSC 503 Seminar

Amy Bucklaew; Renee Miller, PhD - PhD Candidate; Professor, BCS

Faculty Evaluators:  Adam Synder and Ian Fiebelkorn

Student Moderator:  Jo Fritzinger

 Feb 19, 2024 @ 4:00 p.m.

 Medical Center | K-307

NSC 503 Seminar

Daulton Myers; Jeeyun Kim - PhD Candidate

Faculty Evaluators:  Nathan Smith and Manuel-Gomes-Ramirez

Student Moderator:  Mike DuHain

 Feb 12, 2024 @ 4:00 p.m.

 Medical Center | K-307

NSC 503 Seminar

Erin Murray; Margaux Masten - PhD Candidate

Faculty Evaluators:  Juile Fudge and Manoela Fogaça

Student Moderator:  Caitlin Sharp

 Feb 05, 2024 @ 4:00 p.m.

 Medical Center | K-307

Connecting the dots between brain microstructure, function, and behavior in rodents using MRI

Tanzil Arefin, PhD - Assistant Research Professor, Biomedical Engineering, Penn State College of Engineering

 Jan 30, 2024 @ 4:00 p.m.

 Medical Center | Ryan Case Method Rm. (1-9576)

Host: Department of Neuroscience and the Del Monte Institute for Neiroscience

NSC 503 Seminar

Abigail Alpers; Tanique McDonald - PhD Candidate

Faculty Evaluators:  Michael Telias and Andrew Wojtovich

Student Moderator:  Matt Adusei

 Jan 29, 2024 @ 4:00 p.m.

 Medical Center | K-307

NSC 503 Seminars

Dominic Bunn; Mariah Marrero - PhD Candidate

Faculty Evaluators:  Liz Romanski and John Olschowka

Student Moderator:  Sarah Yabonski

 Jan 22, 2024 @ 4:00 p.m.

 Medical Center | K-307

Notter Lecture - The Bodily Senses

Fan Wang, PhD - Professor, Brain and Cognitive Sciences, McGovern Institute, MIT

The bodily sensations include touch, pain, and our senses of the body's postures and movements.  In this talk, I will describe mostly unpublished studies from my lab on neurons and circuits involved in these bodily senses. 

First, I will describe our discovery of touch neurons that tell the brain "when" is the touch onset with millisecond precision.  This is achieved using in vivo recording from ontogenetically identified touch neurons.  Second, I will talk about pain, both the sensory-discriminative and the emotional aspects of pain.  Note that emotions are inseparable from the body's autonomic responses, and we have discovered that controlling autonomic reactions can control pain perception.  Finally, I will discuss our new studies on how the brain (the sensory cortex) represents body postures and associated behaviors.  We have developed new algorithms that enables full parameterized description of the body postures in freely moving mice, and combined with in vivo recording, we have discovered posture/behavior syllable-related ensembles in the brain.

 Jan 17, 2024 @ 4:00 p.m.

 Medical Center | Ryan Case Methods Rm (1-9576)

Host: University of Rochester School of Medicine and Dentistry Department of Neuroscience and the Del Monte Institute for Neuroscience