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TIR microscope

Ermolenko Lab

Structural Dynamics of Translation

Image of the x-ray crystal structure of bacterial 70S ribosome

X-ray crystal structure of
bacterial 70S ribosome

Our research is predominately focused on the mechanism of protein synthesis. The ribosome is a central component of cell metabolism, an extremely complex and highly dynamic machine. We are interested to learn how the structural dynamics of the ribosome and ribosomal ligands enable protein synthesis. We also investigate the folding and dynamics of mRNA molecules, including mRNAs containing riboswitches or structural elements that induce programmed ribosomal frameshifting.

To study structural dynamics of macromolecules we use ensemble and single molecule fluorescent methods. Our main experimental approaches are based on Förster resonance energy transfer (FRET). We follow the kinetics of conformational changes in macromolecules by combining FRET with stopped-flow fast-mixing experiments. We measure FRET in single molecules using total internal reflection fluorescent (TIR) microscopy. 

Dmitri N. Ermolenko, Ph.D.

Dmitri N. Ermolenko, Ph.D.

Principal Investigator

Publications

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News

  • Congratulations to Christine Lai on receiving the Hooker Dissertation Fellowship for 2018-2019 from the University of Rochester!
  • Congratulations to Christine Lai who received the RNA Society Travel Award (to attend the Annual RNA Society Meeting at UC Berkeley in May 2018) and the Sayeeda Zain Travel Award (to attend the Cold Spring Harbor Laboratories Translational Control Meeting in September 2018)!
  • Congratulations to Chen Bao on passing PhD qualifying exam!

Contact Us

  Ermolenko Lab
MC 3-8560
601 Elmwood Ave
Rochester, NY 14642

 (585) 273-3321

  (585) 271-2683