T Cell Studies in Patients Treated with TNF Antagonist
Our project is focused on understanding the T cell immune response in immunocompromised patients treated with anti-TNF-a. Immunocompromised individuals may be more susceptible to infections because of defects in expression of particular immune effector functions during natural infection, and may also fail to generate protective immunity in response to standard vaccination methods.
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Effector Functions of Influenza-Specific T Cells Induced by Immunization and Infection
CD4 T cells are typically classified into different subsets based upon cytokine expression, and hence functionality. However, the diversity of the cytokine patterns within a given subset, Th1 for example, is still quite heterogeneous. There are several mechanisms, not mutually exclusive, that could lead to the observed heterogeneity in an otherwise pre-committed subset within a T helper population.
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Chemokine Expression During the Cell-Mediated Immune Response
CD4 helper T cells elicit their immune regulatory function through the secretion of cytokines and chemokines. The amount and timing of cytokines secreted, which is highly correlated with the immune protection, are tightly regulated during the immune response. We are using influenza vaccination as a model to characterize the secretion of chemokines during the immune response in healthy adults.
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Flow Cytometry Data Analysis Methodologies
Cytometry, especially flow cytometry, is essential for modern immunology (and other biological disciplines). Despite the facts that flow cytometry has been in existence for decades and that automated methods for flow cytometry data analysis have been in the flow cytometry literature since the 1980s, by far manual gating has been the dominant practice.
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CD4 T Cells Derived Amphiregulin in Tissue Repair and Remodeling
During the chronic inflammatory immune response, massive effector mediators derived from inflammatory cells caused severe tissue damage. Meanwhile, the process of tissue repair and remodeling are actively going on, in some cases, causes the pathogenesis of certain diseases, such as asthma. Amphiregulin (AR) is a member of the Epidermal Growth Factor (EGF) family.
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