UR Genomics Research Center

The UR Genomics Research Center (GRC) provides collaborative assistance with experimental design and data analysis for investigators using high-throughput next generation sequencing (NGS), genotyping and gene expression in their research programs.

Services include:

• RNA-Seq
• Dual RNA-seq
• Bar-seq
• CHiP-Seq
• ATAC-seq
• Methyl-Seq
• Small RNA-Seq
• REP-Seq
• Targeted and whole exome sequencing
• Whole genome sequencing
• 16S rRNA microbiome sequencing
• Metatranscriptomics
• Sanger sequencing
• qRT-PCR
• Affymetrix microarrays
• Purification of RNA/DNA
• Custom applications and approaches

In addition, GRC staff lead collaborative projects with URMC investigators to develop new methodologies and incorporate emerging genomic technologies into faculty research programs and the GRC workflow. Dedicated computational support for hardware, data analysis and storage of high-throughput sequence data is provided by the Center for Integrated Research Computing (CIRC).

The following major equipment is located in the GRC:

• Illumina HiSeq2500v4 high-throughput DNA sequencer
• Illumina MiSeq DNA sequencers, one C1 Single-Cell Auto Prep System
• 10X Genomics Chromium Controller
• Sage Science Pippin DNA size fractionation system
• QX100 Droplet Digital PCR System
• ABI QuantStudio 12K Flex Real-Time PCR System with autoloader and microfluidic card module 
• ABI 9700 PCR machines
• BioRad DNA Engine PCR machines
• BioRad C1000 Thermocyclers, 1 Eppendorf 5070 liquid handling robot for qPCR setup
• Eppendorf 5075 liquid handling robot for library construction automation
• Covaris S2 sonication system, one Agilent 2200 TapeStation System
• Agilent 2100 Bioanalyzers
• BioRad gel documentation system
• Qubit flourometer
• Multiple NanoDrop ND-1000 spectrophotometers