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Fees/ Billing

Nucleic Acid

Quality Assessment

**All samples will be assessed for concentration and quality.**
May include NanoDrop, Qubit, Agilent Bioanalyzer, Agilent TapeStation, and/or Agilent Fragment Analyzer (GRC discretion)

All sample types excluding Small RNA - $15/sample
Small RNA - $80/chip (up to 10 samples)

RNA Extraction/Purification

Trizol RNA Extraction - $32.00/sample*
Qiagen RNeasy Plus - $32.00/sample*
Dnase Treatment - $30.00/sample*

* Does not Include quality assessment

DNA Extraction/Purification

Qiagen DNeasy Kit - $27.00/sample*
* Does not Include quality assessment

DNA + RNA Extraction/Purification

Qiagen ALLPREP RNA + DNA - $50.00 per sample*

* Does not Include quality assessment

Next Generation Sequencing

All pricing effective for projects submitted after July 1, 2019 and subject to change.  

Prices reflective of University of Rochester internal usage, external pricing is available.   Contact us for more information.


Library Construction (Commonly requested):

Library Type

Utility

Price/Library

TruSeq mRNA Stranded Transcriptomics $225.00
TruSeq RNA v2 Transcriptomics $225.00
Low Input RNA* Transcriptomics $315.00
TruSeq Total RNA Stranded (H/M/R) Transcriptomics, removes host rRNA only $300.00
TruSeq Total RNA Stranded GOLD (H/M/R) Transcriptomics, removes host rRNA +  mitochondrial rRNA $300.00
NEBNext Small RNA small RNA detection (<200nt), such as micro RNA $180.00
TruSeq PCR-free Whole Genome Sequencing (small + large genomes) $294.00
Nextera FLEX Whole Genome Sequencing (small + large genomes) $120.00
NexteraXT Whole Genome Sequencing (small genomes) + amplicon sequencing $95.00
Whole Genome Bisulphite (WGBS) Epigenetics $350.00
Reduced Representation Bisulphite (RRBS) Epigenetics $350.00
Agilent SureSelect Exome Whole Exome Sequencing (WES) $450.00
ATAC-Seq** Epigenetics $105.00
ChIP-Seq*** Protein:DNA interaction $150.00
16S rRNA  Microbiome $40.00

 

 

 

 

 

 

 

 

 

* *Includes cDNA amplification and NexteraXT library construction.

** Does not include sample-specific procedure optimization.

*** NGS library construction only.  We accept sheared/fragmented ChIP purified DNA only (200-600bp size range).

Note: All library prices listed include library QC, normalization, and pooling unless otherwise indicated.  Other libraries type are available upon request.  Contact us for additional information.

Single-Cell RNAseq (scRNAseq):

10X Genomics - Contact us for pricing

  • Transcriptome wide analysis (5` and 3` variants)
  • Cell Hashing/barcoding
  • CITE-SEQ
  • Immune Profiling (T- and B-cell V(D)J)
  • ATAC-Seq 
  • Linked Reads for Whole Genome Sequencing or Whole Exome Sequencing 

Fluidigm C1 - Contact us for pricing

  • Transcriptome wide analysis
  • ATAC-Seq
  • Targeted resequencing
  • Whole exome Sequencing

Flow Cytometry  - Contact us for pricing

  • SMART-Seq2
  • SCRB-Seq
  • CEL-Seq2
  • Combinatorial indexing (SCI-Seq)
  • other variations available upon request

 

Sequencing:

Illumina NovaSeq6000

Type

Read Length (nt)

Unit

Unit Type

Unit Output (raw clusters in Millions)

Price

Price/25M Clusters

Single-end 100 Flow Cell SP 650M $2,100.00 $80.76
Single-end 100 Flow Cell S1 1300M $4,200.00 $80.76
Single-end 100 Flow Cell S2 3300M $9,726.00 $73.68
Pair-end 50 Flow Cell SP 650M $2,100.00 $80.76
Pair-end 50 Flow Cell S1 1300M $4,200.00 $80.76
Pair-end 100 Flow Cell SP 650M $4,313.00 $165.00
Pair-end 100 Flow Cell S1 1300M $5,963.00 $114.67
Pair-end 50 Flow Cell S2 3300M $9,726.00 $73.68
Pair-end 100 Flow Cell S2 3300M $13,138.00 $99.53
Pair-end 150 Flow Cell S4 8000M $28,000.00 $87.50
Pair-end 250 Flow Cell SP 650M $5,963.00 $229.35

***Users may request preference for flow cell type, however, actual choice will be determined by GRC staff based on sequencing queue to ensure efficient operation of the sequencer.  Users will be billed for flow cell type utilized for their samples.

Illumina NextSeq550

Type 

Read Length (nt) 

  Unit   

Unit Type             

Unit Output

(raw clusters in Millions)

Price

Price/25M clusters

Single-end 75 Flow Cell  Mid-Output >130M $1,502.00 $300.40
Single-end 75 Flow Cell  High-Output >400M $1,800.00 $112.50
Pair-end 75 Flow Cell  Mid-Output >130M $1,502.00 $300.40
Pair-end 75 Flow Cell  High-Output >400M $3,000.00 $187.50
Pair-end 150 Flow Cell Mid-Output >130M $1,502.00 $300.40
Pair-end 150 Flow Cell High-Output >400M $4,810.00 $300.63

***Users may request preference for flow cell type, however, actual choice will be determined by GRC staff based on sequencing queue to ensure efficient operation of the sequencer.  Users will be billed for flow cell type utilized for their samples.

Illumina MiSeq

Type

Read Length (nt)

Unit

Unit Output (raw clusters in Millions)

Price

Pair-end 150 Flow Cell 15M $1,600.00
Pair-end 250 Flow Cell 15M $1,800.00
Pair-end 300 Flow Cell 22M $2,100.00
Library QA/QC 50 Flow Cell 1M $350.00

Contact us for information regarding run options for your given application  Turnaround time depends on sample queue at time of submission and is subject to change based on demand.

** Sequencing costs include basic data processing and preliminary analysis at time of sequencing:

  • Demultiplexing
  • Read processing (filtering based on complexity and end trimming based on quality scores)
  • Alignment to reference genome 
  • Base analysis relevant to library type (e.g., Differential expression analysis for RNA-Seq)

Additional analysis support can be provided. Contact us for more information.

***Additional fees may apply for analysis of data not generated by the GRC*** 

**Turnaround time is based on date of sample receipt and subject to change based on sample QA/QC results.**

Data Analysis

Additional analyses or reanalysis of data is available.

Project Type

Service

Price/Project

RNA-Seq Differential Expression Analysis $271.00
RNA-Seq Isoform Analysis $475.00
RNA-Seq microRNA Analysis $271.00
RNA-Seq Single-Cell Analysis with Cell Ranger $350.00
Whole Genome Sequencing Variant Analysis $407.00
Whole Exome Sequencing Variant Analysis $407.00
ATAC-Seq Peak Enrichment Analysis $543.00
ChIP-Seq Peak Enrichment Analysis $339.00
Methly-Seq Differential Methylation Analysis $550.00
16S rRNA OTU Analysis $475.00
Hourly Rate Custom $68.00
IPA Access Per Hour Charge (login time) $90.00

Quantitative PCR

Service Cost Quantity
ABI High Capacity Reverse Transcription $15.00 Sample
Nugen WT-Ovation PicoSL Kit (RNA Amplification) $86.00 Sample
QPCR Half Plate Setup 384
*up to 192 wells, includes no RT and no template controls, assays not included
$369.00 Half Plate
QPCR Full Plate Setup_384
*up to 384 wells, includes no RT and no template controls, assays not included
$574.00 Full Plate
QPCR Half Plate Setup_96
*up to 48 wells, includes no RT and no template controls, assays not included
$247.00 Half  Plate
QPCR Full Plate Setup_96
*up to 96 wells, includes no RT and no template controls, assays not included
$317.00 Full  Plate
ABI QuantStudio Instrument Run Fee $60.00 Plate
ABI Microfluidic Card (TLDA) Set-up And Run On ABI QuantStudio (Does not include TLDA) $80.00 TLDA

 

Micro Array Analysis  

 Contact us for pricing.