GRC Resource Descriptions for Grant Proposals
Facilities & Resources
UR Genomics Research Center
The UR Genomics Research Center (GRC), located at the James P. Wilmot Cancer Institute, provides core laboratory support, technical consultation, assistance with experimental design and data analysis for investigators using high-throughput genomic sequencing, genotyping and gene expression in their research programs. The GRC currently provides support for RNA-Seq, CHiP-Seq, Exome/Genome sequencing, Single-cell genomics applications, ATAC-Seq, whole genome and reduced representation bisulfite sequencing (WGBS, RRBS), 16S rRNA sequencing, qRT-PCR, Affymetrix microarrays, processing of RNA/DNA, as well as custom applications and approaches. In addition, GRC staff lead collaborative projects with URMC investigators to develop new methodologies and incorporate emerging genomic technologies into the GRC workflow. The GRC has a faculty-level director, a faculty-level Co-Director, a Lab Manager, one Technical Scientist, one Senior Technician, two Technical Associates, two Technicians and six Bioinformaticians/data analysts. Dedicated computational support for hardware, data analysis and storage of high-throughput sequence data is provided by the Center for Integrated Research Computing (CIRC) with 37 24-core compute nodes and 300TB of space dedicated solely for GRC analyses.
The following major equipment is located in the GRC: one Illumina NovaSeq6000 high-throughput DNA sequencer, one Illumina NextSeq550 high-throughput DNA sequencer, two Illumina MiSeq DNA sequencers, one Fluidigm C1 Single-Cell AutoPrep System, two 10X Genomics Chromium Controllers, one QuantStudio 12K FLEX real-time PCR instrument with autoloader, microfluidic card module, and open array module for digital PCR, one BioRad QX100 digital droplet PCR system, one Sage Science PippinPrepHT size fractionation system, one Sage Science PippinPrep size fractionation system, one Covaris E220 Focused-ultrasonicator, two ABI 9700 PCR machines, six BioRad DNA Engine PCR machines, one Perkin Elmer Sciclone G3 liquid handler, one Eppendorf EpMotion 5070 liquid handler, one Eppendorf EpMotion 5075 liquid handler, two Agilent 2100 Bioanalyzers, one Agilent TapeStation2200, one Agilent Fragment Analyzer, one BioRad gel documentation system, one Qubit Flourometer, and multiple NanoDrop ND-1000 spectrophotometers.
Please contact theGRC with any questions or to receive specific methods for your project.