GRC Descriptions for Grant Proposals and Data Management
Facilities & Resources
UR Genomics Research Center
The UR Genomics Research Center (GRC), located at the James P. Wilmot Cancer Institute, provides core laboratory support, technical consultation, assistance with experimental design and data analysis for investigators using high-throughput genomic sequencing, genotyping and gene expression in their research programs. The GRC currently provides support for RNA-Seq, CHiP-Seq, Exome/Genome sequencing, Single-cell genomics applications, ATAC-Seq, whole genome and reduced representation bisulfite sequencing (WGBS, RRBS), 16S rRNA sequencing, qRT-PCR, Affymetrix microarrays, processing of RNA/DNA, as well as custom applications and approaches. In addition, GRC staff lead collaborative projects with URMC investigators to develop new methodologies and incorporate emerging genomic technologies into the GRC workflow. The GRC has a faculty-level Director, a Lab Manager, one Technical Scientist, one Senior Technician, two Technical Associates, two Technicians and six Bioinformaticians/data analysts. Dedicated computational support for hardware, data analysis and storage of high-throughput sequence data is provided by the Center for Integrated Research Computing (CIRC) with 37 24-core compute nodes and 300TB of space dedicated solely for GRC analyses.
The following major equipment is located in the GRC: one Illumina NovaSeq6000 high-throughput DNA sequencer, one Illumina NextSeq550 high-throughput DNA sequencer, two Illumina MiSeq DNA sequencers, one Fluidigm C1 Single-Cell AutoPrep System, two 10X Genomics Chromium Controllers, one QuantStudio 12K FLEX real-time PCR instrument with autoloader, microfluidic card module, and open array module for digital PCR, one BioRad QX100 digital droplet PCR system, one Sage Science PippinPrepHT size fractionation system, one Sage Science PippinPrep size fractionation system, one Covaris E220 Focused-ultrasonicator, two ABI 9700 PCR machines, six BioRad DNA Engine PCR machines, one Perkin Elmer Sciclone G3 liquid handler, one Eppendorf EpMotion 5070 liquid handler, one Eppendorf EpMotion 5075 liquid handler, two Agilent 2100 Bioanalyzers, one Agilent TapeStation2200, one Agilent Fragment Analyzer, one BioRad gel documentation system, one Qubit Flourometer, and multiple NanoDrop ND-1000 spectrophotometers.
NIH has issued the Data Management and Sharing (DMS) policy (effective January 25, 2023) to promote the sharing of scientific data. Sharing scientific data accelerates biomedical research discovery, in part, by enabling validation of research results, providing accessibility to high-value datasets, and promoting data reuse for future research studies.
In partnership with Miner Library, the GRC has developed template Data Management Plans and a Data Size Estimator to help support researchers with their grant submissions.
Data Size Estimation Tool*
*This estimation tool is meant as a guide and is based on the project deliverables provided by the GRC. While we attempted to slightly overestimate the storage need for each project types and consider as many scenarios as possible, there may be situations or circumstances (e.g., custom library type or sequencing) where this estimator provides an underestimate. We strongly encourage consulting the GRC is any questions arise or to help with finalizing any information pertaining to your project.
Template DMPs for common GRC services:
10X scRNA Sequencing: GRC_DMP_10XscRNAseq_012023
Bulk RNA Sequencing: GRC_DMP_Bulk_RNAseq_012023
Whole Genome Sequencing: GRC_DMP_WGS_WES_012023
ChIPseq/ATAC Sequencing: GRC_DMP_ChIPseq_ATACseq_012023
CUTandRUN/CUTandTAG Sequencing: GRC_DMP_CUTandRUN_CUTandTAG_012023
Metagenome and Metatranscriptome Sequencing: GRC_DMP_MGX_MTX_DMP_112823
Need help with High-throughput sequencing Data Storage and deposition to NIH public repositories?
We can help, contact us with questions on how and cost. GRC policy is to archive all data we generate for a period of 5-year, longer in many cases. We have access to local and cloud-based storage solutions approved for use by University IT that includes high risk/legally restricted data. If you would like us to help with data deposition, it is much easier for us to to also store these data on our secure systems.
Please contact theGRC with any questions or to receive specific methods or estimated storage sizes for your project.