Seminars & Workshops
10X Genomics: Sample Preparation for Single Cell Analysis: Basics and Beyond
October 14th, 4-5 pm Medical Center K-207
Presented By: Patrick Murphy, PhD
The vast complexities of biology require approaches that build a complete picture from single
cells to tissues and beyond. Single cell analysis is a powerful technique to characterize
complex tissue types, identify rare cell populations, uncover regulatory relationships between
genes, and track cell trajectories. From sample handling to cell dissociation, there are a
number of technical considerations that influence cell viability and data quality of single cell
genomics, single cell epigenomics, and single cell gene expression experiments. Join us for
this seminar, which covers critical sample prep decisions and technical considerations for
single cell assay design and optimization.
Snacks will be provided.
CLICK HERE TO REGISTER
Previous Seminars + Workshops:
Webinar with Advanced Cell Diagnostics to discuss the use of RNAscope with 10X and GeoMx Spatial Platforms
June 8th, 1pm
Introduction to Single Cell RNAseq Analysis Workshop
The Genomics Research Center is hosting an informatics workshop series: Introduction to single cell RNAseq analysis. The series will have four sessions, and each session will be split into a 45 minute lecture followed by a hands-on computational workshop that builds on the information covered in the lecture. Please note that attendance to the lecture is a prerequisite for the hands-on workshop that day.
Monday May 2, 2022
1:00 pm - 1:45 pm Lecture: Experimental Design and Wet Lab Considerations for Designing a Successful scRNA-Seq Study
2:15 pm - 4:30 pm Workshop: Introduction to R, Rstudio, and the Seurat package
Tuesday May 3, 2022
1:00 pm - 1:45 pm Lecture: scRNAseq quality control and clustering methods of single cell RNAseq data in Seurat
2:15 pm - 4:30 pm Workshop: QC methods and interpretation; basic cell clustering
Thursday May 5, 2022
1:00 pm - 1:45 pm Lecture: Cell typing, cell markers, and differential expression in Seurat
2:15 pm - 4:30 pm Workshop: Cell typing and delineating cell markers that define clusters
Friday May 6, 2022
1:00 pm - 1:45 pm Special Topics Lecture: GRC team will address individual questions
2:15 pm - 4:30 pm Special Topics Workshop: GRC team will demonstrate scRNAseq computational methods of interest to attendees
A pre-workshop connectivity session is scheduled for Tuesday April 26th 2:00 pm - 3:30 pm. A zoom link will be sent upon registration. If you do not have experience with R or Rstudio, here is a tutorial to get you started on the basics: https://swirlstats.com/students.html
Register here: https://redcap.urmc.rochester.edu/redcap/surveys/?s=XC79FEY9KK4PWW9Y
GRC + T32 Immunology Workshops:
The GRC is currently working to provide bioinformatics analysis training workshops for T32 immunology trainees. The sessions cover basic skills in R and offer practical experience with commonly used bioinformatics analysis packages in R.
Single cell and spatial transcriptomic methods to understand cancer
Hosted by the Genomics Research Center for the University of Rochester
Thursday March 10, 2022
1:00 - 1:50 PM ET
Patrick Murphy, PhD - Science and Technology Advisor for 10X Genomics
The vast complexities of cancer are characterized by heterogeneity across samples,
from tumor cells and tumor microenvironments to therapeutic responses. Elucidating
disease mechanisms requires a deep understanding of these complexities. However,
traditional assays and tools that analyze tissue in bulk miss significant amounts of
information and context due to limited throughput and/or resolution. Through
innovations in single cell sequencing and spatial transcriptomics, solutions from 10x
Genomics help researchers investigate the body’s response to tumors, discover
tumor-associated mutations, and uncover mechanisms of acquired resistance to
therapy. Join 10x Genomics to learn how researchers are using our single cell assays
and spatial tools to gain a multidimensional view of cancer.
Contact the GRC for a copy of the live recorded webinar!
Click here to reach out to the GRC for access to this recorded webinar
- Discovery novel protein and RNA biomarkers through spatial enrichment
- Interrogate regions of interest and segment compartments across your sample
- Access fresh, frozen and archived FFPE to fully profile critical spatial biology
- Whole Transcriptome, 100s of Proteins analyzed with NGS readout
Contact us for the recorded webinar!