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URMC / Labs / Ovitt Lab / Mouse Models

Mouse Models Generated or Used in the Ovitt Laboratory

Ascl3EGFP-Cre

The Ascl3 (achaete scute-like 3) gene locus consists of two exons, and the entire coding sequence lies within the second exon. A knock-in construct was generated which replaces the second exon with an expression cassette encoding EGFP and Cre recombinase as a fusion protein. This construct was targeted to the Ascl3 locus by homologous recombination, placing EGFP-Cre under control of the Ascl3 promoter. The neomycin gene used for ES cell targeting is flanked by LoxP sites (black) and was subsequently removed by Cre recombination. This strain is available from The Jackson Laboratory.

https://www.jax.org/strain/021794

Bullard et al. (2008) Ascl3 expression marks a progenitor population of both acinar and ductal cells in mouse salivary glands. Dev Biol 320:72-78.

PipGCE

The Pip (prolactin-induced protein) gene (gene ID 18716) was targeted by homologous recombination with a fusion cassette encoding GFP and CreERT2 (GCE) to remove the coding sequences from Exon 1 and place the GCE cassette under the control of Pip regulatory sequences. This strain is available from The Jackson Laboratory.

https://www.jax.org/strain/023201

Maruyama et al.  (2016) Cell-specific Cre strains for genetic manipulation in salivary glands. PLOS One, 2016 Jan 11;11(1):e0146711. doi: 10.1371/journal.pone.0146711

Dcpp1GCE

The GCE fusion cassette (encoding GFP and CreERT2) was inserted into the Dcpp1 (demilune cell and parotid protein) gene locus (gene ID 13184) at the initiation site in Exon 2 through homologous recombination. Expression of the GCE cassette is under the control of Dcpp1 regulatory sequences. This strain is available from The Jackson Laboratory.

https://www.jax.org/strain/028731

Maruyama et al.  (2016) Cell-specific Cre strains for genetic manipulation in salivary glands. PLOS One, 2016 Jan 11;11(1):e0146711. doi: 10.1371/journal.pone.0146711

Tcfcp2l1GCE

The Tcfcp2l1 gene (gene ID 81879) was targeted with the GCE fusion cassette at the initiation codon in Exon 1. The GCE cassette was inserted by homologous recombination and is expressed under the control of Tcfcp2l1 regulatory sequences. This strain has been deposited with The Jackson Laboratory.

https://www.jax.org/strain/028732

Maruyama et al.  (2016) Cell-specific Cre strains for genetic manipulation in salivary glands. PLOS One, 2016 Jan 11;11(1):e0146711. doi: 10.1371/journal.pone.0146711