Welcome to the URMC Pathway Discovery Resource The Pathway Discovery Resource (PDR) is a high throughput screening facility providing cost effective screening methodologies for cell based assays. Currently, the PDR offers cell based screening with the Silencer® Select Human Druggable Genome siRNA Library V4 and three chemical compound libraries (Spectrum, Chembridge, and Prestwick). The following major equipment is located in the PDR: one Celigo S Cell cytometer, one Perkin Elmer Zephyr compact liquid handling workstation, ThermoFisher MultiDrop, one Envision High Throughput Plate Reader equipped with optics for Fluorescence, Luminescence, Fluorescence Polarization (FP), Time Resolved Fluorescence (TRF), Absorbance, Alpha Screen, and FRET based detection in 96 or 384 well formats, one Janus dual-arm robotic liquid handling system, and one Flexdrop plate filler. The PDR is Co-directed by two Faculty-level Instructors experienced in high throughput cell and molecular biology techniques. In addition, the PDR is operated by a full time Technical Associate with significant experience in high throughput technologies. The PDR integrates with various other shared resource facilities at University of Rochester including; genomics, flow cytometry, and proteomics. Note: The Pathway Discovery Resource is funded, in part, through the University of Rochester Developmental Center for AIDS Research.