Instrumentation The Pathway Discovery Resource (PDR) has obtained state-of-the-art equipment for assay development and medium to high throughput Screening. Envision High Throughput Plate Reader The heart of a good screen is a good assay. The envision plate reader is a high sensitivity plate reader suitable for analysis of many types steady state high throughput assays that allows users a high level of flexibility in assay development. The reader is equipped with optics that allow for measurement of Fluorescence, Luminescence, Fluorescence Polarization (FP), Time Resolved Fluorescence (TRF), Absorbance, Alpha Screen, FRET and other assays in 96 or 384 well formats. The Laboratory has purchased the appropriate optics for many of these assays and the capabilities can be expanded with purchase of other optical modules depending on the needs of the investigator. Robotic Liquid Handling System The Janus dual arm robot is suitable for high throughput screening of large chemical libraries and cherry picking of hits from the library for titration and hit validation. The robot is equipped with an 8 position pipetting head for hit selection, a 96 tip head for screening and a 384 position pin tool for screening in 384 well plates. The robot is also equipped with a bar coded plate feeder and a gripper arm for moving plates on and off the deck. Flexdrop Plate Filler Used to routinely fill plates with reagent components required for screening assays. Can dispense protein reagents or cultured cells into 96 or 384 well plates. These would then be moved to the robotic liquid handling system for compound addition. Zephyr® Compact Liquid Handling Workstation The Zephyr is a precision liquid handling instrument. The multi-channel liquid handling workstation can handle applications in areas including compound management, high throughput screening (HTS), genomics and proteomics. MultiDrop ™ Combi Reagent Dispenser Thermo Scientific™ Multidrop™ Combi Reagent Dispenser is an automatic, programmable, eight-channel microplate bulk reagent dispenser for microvolume dispensing. It has a peristaltic pump for rapid and continuous dispensing of liquids into various microplates. Multidrop Combi offers outstanding flexibility for a wide range of applications including drug discovery/highthroughput screening, genomic, proteomic and cell-based assays, and ELISA. The Multidrop is capable of dispensing volumes from 0.5 to 2500 µl for 96, 384 and 1536-wells. Celigo S Image Cytometer Nexcelom’s Celigo® S is a bench-top, micro-well plate based, bright field and fluorescent imaging system that allows for the fully automated imaging and quantification of suspension, adherence cells, tumor spheroids, iPSC and cancer stem cell colonies within 6, 12, 24, 48, 96, 384, 1536-well plates, T flasks, and slides. For instrument specifications, visit the Nexcelom site.