Validation of Gene Array to Predict Bacterial Co-infection In Adults Hospitalized with Viral Lower Respiratory Tract Infections (LRTI) (Mariani/Fasley)
The primary goal of the current innovation project is to conduct analysis of the RNASeq data collected in DMID 13-0070, in an effort to identify gene expression variables capable of classifying, or predicting, bacterial and viral infection in adults with LRTI. The secondary goal is to perform gene expression validation of previously identified and new predictors.
Serum Biomarkers associated with bacterial respiratory infections in hospitalized adults (Falsey)
This project will utilize serum banked from prior NIH funded project 1R01AI079446-01 “Reduction of Unnecessary Antibiotics in Adults by the Use of Viral Diagnostics” In this study, blood was prospectively collected at time of admission and 4 weeks after illness resolution from subjects hospitalized with respiratory illness. Each illness had extensive microbiologic testing performed in order to categorize illnesses as bacterial, viral or mixed bacterial and viral. The proposed project will utilize these banked sera and test the utility of 3 newly identified serum biomarkers to differentiate bacterial and viral respiratory infection. Informed consent was obtained in the original grant to test serum biomarkers for this purpose.
Testing of Sputum by Film Array for viral and atypical bacterial respiratory pathogens (Falsey)
The purpose of this project was to develop a simple method to test sputum in a fully automated PCR system. Archived samples from R01AI079446-01 were used. The work was consistent with the original intent and consent of the study to develop better tests for diagnosis of viral and bacterial infection.
Testing for an impaired function of cord blood T regulatory cells from preterm neonates who are predisposed to developing severe respiratory viral infection versus gestational age-matched controls (Misra)
The overall goal of the project is to provide a manuscript at the end of the study period, which describes the function of T regulatory cells from the cord blood of infants who are born preterm in the presence of inflammation of the umbilical cord and those who develop the lung disease Bronchopulomnary Dysplasia.
Pneumococcal Colonization in Older Adults (Falsey)
Determine the cumulative incidence of pneumococcal carriage over an 8 month period of time in persons > 65 years of age. Evaluate potential variables that influence the risk of carriage. Depending on the cumulative incidence the following secondary objectives will be explored. Samples will be collected and stored in such a way to allow future testing as warranted.