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GRC Sequencing Instrumentation

Illumina NovaSeq 6000 Sequencer

Learn more about the NovaSeq6000 system 

NovaSeq Instrument Image ​    NovaSeq Specifications by Flow Cell

NovaSeq 6000 Performance Specifications

NovaSeq Applications:

  • Whole Genome Sequencing
  • Whole Exome Sequencing
  • Whole Transcriptome Sequencing

Illumina NextSeq 550

Learn more about the NextSeq 550 System 

NextSeq Instrument ImageNextSeq Instrument Specifications by Flow Cell

NextSeq 550 Performance Specifications

NextSeq 550 Applications:

  • Gene Expression Profiling
  • Exome Sequencing
  • Whole Genome Sequencing

Illumina MiSeq 

Learn more about the MiSeq System

MiSeq InstrumentMiSeq Specifications by Reagent Kit Type

MiSeq performance specifications

MiSeq Applications:

  • Amplicon Sequencing
    • 16s (microbiome)
    • Targeted/Custom Panels
      • Rep-seq
      • Targeted Amplicon Panels
        • TruSeq Cancer Amplicon (LINK)
        • TruSight Myeloid Panel (LINK)
  • Small Genome Sequencing
    • Bacteria, Viruses, etc.

Recommended Reads Per Library:

Application Reads per Library
mRNA-Seq 20 - 25 Million
Total RNA-Seq 40 - 50 Million
Small RNA-Seq 20 Million
Low Input RNA-Seq 20 - 25 Million
Chip-Seq 15 Million
ATAC-Seq 50 Million
Whole Genome (10X) 125 Million Pairs
Whole Genome (4X) 50 Million Pairs
Whole Genome Bisulfite-Seq (10X) 125 Million Pairs
Whole Exome Sequencing (100X) 50 Million Pairs
Single Cell (10X v3) 100,000 per Cell


**The above recommendations are based on Human/Mouse/Rat genomes for simplicity and empirical results from GRC experience.  Other organisms may require different sequencing depths (more or less samples per lane).  The amount of sequencing performed and the type (single vs pair) is organism and application specific.  These recommendations are guidelines and by no means meant to be strict cutoffs.  Internal GRC data and cumulative data analysis over the past formed the basis for these suggestions with the sole purpose to help investigators select the most appropriate experiment type for their research goals.

Comparison of Sequencers at the GRC



NovaSeq, NextSeq, and MiSeq Flow Cell Image

Illumina HiSeq2500 Sequencer - Obsolesced 2019

Learn more about the HiSeq2500 system

Contact us or use the Sequencing Platform Comparison Tool to find the right alternative for your research needs. 

Data Analysis

The GRC currently has 2 bioinformaticians on staff with experience in NGS data analysis methods and workflows.  For more information please visit the GRC Bioinformatics Page or contact us.