The rapid mutation and subsequent tremendous antigenic variation of HIV at the population and individual patient level, coupled with its destruction of CD4 T cells makes it a difficult target for protective vaccine responses. Our laboratory has described key mechanistic features of the B cell response to HIV Envelope among HIV-infected patients, and seeks to develop strategies utilizing diverse animal models for qualitatively tuning the B cell response by vaccination to establish protection from infection.
Learn more about Induction of Protective B Cell Responses to Human Immunodeficiency Virus
The human genome is comprised of approximately 8% genetic material inserted by endogenous retrovirus over millions of year of evolution. Most Human Endogenous Retroviruses (HERVs) are dysfunctional because of numerous mutations and deletions, some however are capable of producing functional transcripts, proteins, and impacting gene regulation.
Learn more about Human Endogenous Retrovirus K - A Novel Target for HIV and Cancer Therapeutics
Through B cell receptor (BCR) sequencing at the single cell and global population level the exact specificity of the B cell response- its origin, potential, and fate can be determined.
Learn more about Advanced Immunoglobulin Repertoire Analysis
In collaboration with Dr. Lisa DeLouise, the Kobie Lab has developed patented nanowell array technology called microbubbles, which allow for the long term culture and functional interrogation of B cells at the single cell level.
Learn more about Microbubble Array Technology – Functional Resolution and Isolation of Single Cells